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Moleculaar-biological analysis of function of chloroplast membrane lopids in highr plants

Research Project

Project/Area Number 14540596
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionThe University of Tokyo

Principal Investigator

WADA Hajime  The University of Tokyo, Graduate School of Arts and Science, Associate Professor, 大学院・総合文化研究科, 助教授 (60167202)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsPhotosynthesis / Arabidopsis thaliana / thylakoid membane / Phosphatidylglycerol / chloroplast / Cyanabacteria
Research Abstract

Phosphatidylglycerol (PG) is a ubiquitous phospholipid in the biological membranes of many organisms. In plants, PG is mainly present in chloroplasts and has been suggested to play specific roles in photosynthesis. However, the specific roles of PG in chloroplasts have not been well understood. In this study, we have investigated the roles of PG in chloroplasts with two T-DNA tagged lines of Arabidopsis thaliana that have a T-DNA insertion in the PGP1 gene encoding a PG phosphate synthase involved in the biosynthesis of PG in chloroplasts. In homozygous plants of the T-DNA tagged lines, the PGP1 gene was completely disrupted. The growth of these knockout mutants was dependent on the presence of sucrose in the growth medium, and these plants had pale yellow-green leaves. The leaves of the mutants had remarkably large intercellular spaces due to the reduction in the number of mesophyll cells. The development of chloroplasts in the leaf cells was severely arrested in the mutants. Mesophyll cells with chloroplast particles are only found around vascular structures, whereas epidermal cells are enlarged but largely conserved. The content of PG in ghe mutants was reduced to 12% of the of the wild type. These results demonstrate that pg is essential for the development of thylakoid membranes In A.thaliana.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Miki Hagio: "Phosphatidylglycerol is essential for the development of thylakoid membranes in Arabidopsis thaliana"Plant and Cell Physiology. 43・12. 1456-1464 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chang cheng Xu: "The pgpl mutant locus of Arabidopsis encodes a phosphatidylglycerol phosphate synthcse with impaired activity"Plant Physiology. 129. 594-604 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Isamu Sakurai: "Requirement of phosphatidylgly cerol for maintenance of photosynthetic machinery"Plant Physiology. 133. 1376-1384 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ildiko' Demonkos: "Phosphatidylglycerol Is Essential for Oligomerization of Photosystem I Reaction Center"Plant Physiology. 134. 1471-1478 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miki Hagio et al.: "Phosphatidylglycerol id essential for the development of thylakoid membranes in Arabidopsis thaliana"Plant Cell Physiology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chang cheng Xu et al.: "Eakin, C., Benning, C. The pgp1 mutant locus of Arabidopsis encodes a phosphatidylglycerolphosphate synthase with impaired activity (Eakin, C., BEnning, C.)"Plant Physiology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Isamu Sakurai eee: "Requirement of phosphatidylglycerol for maintenance of photosynthetic machinery"Plant Physiology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ildiko' Demonkos et al.: "Farkas, t., Gombos, Z, phosphatidylglycerol is essential for oligomerization of PS I reaction center (Farkas, T., Gombos, Z.)"Plant Physiology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miki Hagio: "Phosphatidylglycerol is essential for the development of thylakoid membranes in Arabidopsis thaliana"Plant and Cell Physiology. 43. 1456-1464 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Isamu Sakurai: "Requirement of phosphatidylglycerol for maintenance of photosynthetic machinery"Plant Physiology. 133. 1376-1384 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ildiko Domonkos: "Phosphatidylglycerol is essential for oligomerization of PSI reaction center"Plant Physiology. 134(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Zoltan Gombos: "Phosphatidylglycerol requirement for the function of electron acceptor plastoquinone Q_B in the photosystemII reaction center"Biochemistry. 41. 3796-3802 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chang Cheng Xu: "The Pgp1 mutant locus of Arabidopsis encodes a phasphatidyglycerol phosphate synthare with impaired activity"Plant Physiology. 129. 594-604 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Miki Hagio: "Phosphatidylglycerol is essential for the development of thylakoid membranes in Arabidopsis thaliana"Plant and Cell Physiology. 43. 1456-1464 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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