Mechanisms of eye relocation during flatfish metamorphosis, - asymmetrical morphogenesis by thyroid hormone
| Project/Area Number |
14540614
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物形態・構造
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| Research Institution | Kyoto University |
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Principal Investigator |
TAGAWA Masatomo Kyoto University, Field Science Education and Research Center, Associate Prof., フィールド科学教育研究センター, 助教授 (20226947)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | metamorphosis / left-right asymmetry / body color / eye relocation / flatfish / thyroid hormone / timing in early development / malformation / 甲状腺ホルモン感受性 / タイミング / pseudomesial bar / ヒラメ / カレイ類 / 部域差 / ホシガレイ / retrorbital vesicle |
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| Research Abstract |
The external asymmetry of flatfish is most significant in the animal kingdom. One eye of the symmetrical larva migrates to the opposite side, and the side with both eyes (ocular side) becomes dark by the expression of the adult-type melanophores(aMLP) during metamorphosis, particularly under the control of thyroid hormones(THs).
The pseudomesial bar(Pb), a unique bone that is present in flatfish only on the side without eyes (blind side), simultaneously developed with the progress of eye migration during metamorphosis. Since Pb required TH at a specific time, the involvement of TH was indicated in the formation of the blind side. From in vivo and in vitro experiments, it was suggested that TH also plays a role in the coloration of the blind side by suppressing the spontaneous expression of aMLP.
To answer the question why TH can exert its effect only on the future blind side, the larvae of spotted halibut were artificially metamorphosed by TH treatment at various timings. The occurrence of "ambicoloration" (ocular-side characteristic on both the sides) was increased by metamorphosis under the action of TH that was either too early or too late. On the other hand, "pseudoalbino" (blind-side characteristic on both sides) was observed under the action of TH at a specific time. Based on the results, TH responsiveness to induce blind-side characteristic was estimated. The responsive period was longer for the left side (blind side in the normal juveniles) than for the right side, and this difference was believed to be the central mechanism for the metamorphic asymmetry.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Isolation and characterization of a Japanese flounder clonal line, reversed, which exhibits reversal of metamorphic left-right asymmetry.2002
Author(s)
Hisashi Hashimoto, Akira Mizuta, Nozomi Okada, Tohru Suzuki, Masatomo Tagawa, Kazuo Tabata, Yoshihiro Yokoyama, Morihiko Sakaguchi, Masaru Tanaka, Haruhiko Toyohara.
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Journal Title
Mech.Dev. 1111
Pages: 17-24
Description
「研究成果報告書概要(欧文)」より
Related Report
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