• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular Mechanism Underlying Associative Learning in Mollusk

Research Project

Project/Area Number 14540622
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

ITO Etauro  Hokkaido Univ., Grad.School of Sci., Asso.Prof., 大学院・理学研究科, 助教授 (80203131)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordsleaning / memory / CREB / single-cell analysis / in situ hybridization / immunohistochemistry / qRT-PCR / Lymnaea / C / EBP / mRNA / 味覚嫌悪学習 / スプライシング / NO合成酵素 / 長期促通 / アンチセンス・オリゴヌクレオチド
Research Abstract

When an appetitive stimulus (sucrose) is presented with an aversive stimulus (potassium chloride) to the pond snails Lymnaea stagnahs, associative learning, which is referred to as conditioned taste aversion (CTA), is quickly acquired and persisted for up to a month in snails. Previous results obtained by our cellular analyses showed that the cerebral giant cell is the key neuron for the formation of learning and the consolidation of long-term memory for CTA in L.stagnalis. In the present study, the molecular mechanism of CTA was analyzed with paying attention to a transcription factor. The transcription factor, cyclic AMP responsive element binding proteins (CREB), is considered to play major roles in the consolidation of long-term memory in various animals. We therefore cloned the cDNAs in L.stagnalis and analyzed their mRNA localization in identifiable neurons in the central nervous system. We found that the cerebral giant cells contain these mRNAs. Further, the splicing variants of these genes were studied, and 7 isoforms were found for CREB1 (so called "activator"). However, three of these CREB1 isoforms do not have any potential sites of phosphorylation by protein kinase A in their predicted amino acid sequences. This result suggests that the functions of these 3 isoforms as transcription factors differ from the other 4 isoforms and that all the isoforms affect to each other, resulting in the change in their DNA binding ability. Finally, we succeeded in measuring the changes in copy numbers of the CREB mRNAs in the cerebral giant cells during CTA by single-cell real-time quantitative RT-PCR. The reduction of CREB2 (represser) was found to be essential for the consolidation of long-term memory.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Fujie, S.: "The nitric oxide/cyclic GMP pathway in the olfactory processing system of the terrestrial slug Limax marginatus"Zoological Science. 19. 15-26 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chono, K.: "Non-ocular dermal photoreception in the pond snail Lymnaea stagnalis"Brain Research. 951. 107-112 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ito, I.: "Negative relationship between odor-induced spike activity and spontaneous oscillations in the primary olfactory system of the terrestrial slug Limax marginatus"Zoological Science. 20. 1327-1335 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ito, I.: "Distributions of γ-aminobutyric acid immunoreactive and acetylcholinesterase-containing cells in the primary olfactory system in the terrestrial slug Limax marginatus"Zoological Science. 20. 1337-1346 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sadamoto, H.: "CREB in the pond snail Lymnaea stagnalis : cloning, gene expression, and function in identifiable neurons of the central nervous system"Journal of Neurobiology. 58. 455-466 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ito, I.: "Modulation of two oscillatory networks in the peripheral olfactory system by γ-aminobutyric acid, glutamate, and acetylcholine in the terrestrial slug Limax marginatus"Journal of Neurobiology. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Wagatsuma, A.: "The early snail acquires the learning. comparison of scores for conditioned taste aversion between morning and afternoon"Acta Biologica Hungarica. 55. 149-155 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hatakeyama, D.: "Quantitative real-tame RT-PCR method for estimation of mRNA level of CCAAT/enhancer binding protein in the central nervous system of Lymnaea stagnalis"Acta Biologica Hungarica. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sadamoto, H.: "The expression pattern of CREB genes in the central nervous system of the pond snail Lymnaea stagnalis"Acta Biologica Hungarica. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujie, S.: "Nitric oxide synthase and soluble guanylyl cyclase underlying the modulation of electrical oscillations in a central olfactory organ"Journal of Neurobiology. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujie, S.: "The nitric oxide/cyclic GMP pathway in the olfactory processing system of terrestrial slug Limax marginatus"Zoological Science. 19. 15-26 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chono, K.: "Non-ocular dermal photoreception in the pond snail Lymnaea stagnalis"Brain Research. 951. 107-112 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ito, I.: "Negative relationship between odor-induced spike activity and spontaneous oscillations in the primary olfactory system of the terrestrial slug Limax marginatus"Zoological Science. 20. 1327-1335 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ito, I.: "Distributions of γ-aminobutyric acid immunoreactive and acetylcholineaterase-containing cells in the primary olfactory system in the terrestrial slug Limax marginatus"Zoological Science. 20. 1337-1346 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sadamoto, H.: "CREB in the pond snail Lymnaea stagnalis : cloning, gene expression, and function in identifiable neurons of the central nervous system"Journal of Neurobiology. 58. 455-466 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ito, I.: "Modulation of two oscillatory networks in the peripheral olfactory system by γ-aminobutyric acid, glutamate and acetylcholine in the terrestrial slug Limax marginatus"Journal of Neurobiology. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Wagatsuma, A.: "The early snail acquires the learning. comparison of scores for conditioned taste aversion between morning and afternoon"Acta Biologica Hungarica. 55. 149-155 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hatakeyama, D.: "Quantitative real-tame RT-PCR method for estimation of mRNA level of CCAAT/enhancer binding protein in the central nervous system of Lymnaea atagnalis"Acta Biologica Hungarica. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sadamoto, H.: "The expression pattern of CREB genes in the central nervous system of the pond snail Lymnaea stagnalis"Acta Biologica Hungarica. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujie, S.: "Nitric oxide synthase and soluble guanylyl cyclase underlying the modulation of electrical oscillations in a central olfactory organ"Journal of Neurobiology. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Fujie, T.Yamamoto, J.Murakami, D.Hatakeyama, H.Shiga, N.Suzuki, E.Ito: "Nitric oxide synthase and soluble guanylyl cyclase underlying the modulation of electrical oscillations in a central olfactory organ"Journal of Neurobiology. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] I.Ito, T.Kimura, S.Watanabe, Y.Kirino, E.Ito: "Modulation of two oscillatory networks in the peripheral olfactory system by γ-aminobutyric acid, glutamate and acetylcholine in the terrestrial slug Limax, marginatus"Journal of Neurobiology. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Sadamoto, H.Sato, S.Kobayasbi, J.Murakami.H.Aonuma, H.Ando, Y.Fujito, K.Hamano, M.Awaji, K.Lukowiak, A.Urano, E.Ito: "CREB in the pond snail Lymnaea stagnalis. cloning, gene expression and function in identifiable neurons of the central nervous system"Journal of Neurobiology. 58. 455-466 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] I.Ito, S.Watanabe, T.Kimura, Y.Kirino, E.Ito: "Distributions of γ-aminobutyric acid immunoreactive and acetylcholinesterase-containing cells in the primary olfactory system in the terrestrial slug Limax marginatus"Zoological Science. 20. 1337-1346 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] I.Ito, S.Watanabe, T.Kimura, Y.Kirino, E.Ito: "Negative relationship between odor-induced spike activity and spontaneous oscillations in the primary olfactory system of the terrestrial slug Limax marginatus"Zoological Science. 20. 1327-1335 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Wagatsuma, R.Sugai, K.Chono, S.Azami, D.Hatakeyama, H.Sadamoto, E.Ito: "The early snail acquires the learning. comparison of scores for conditioned taste aversion between morning and afternoon"Acta Biologica Hungarica. 55. 149-155 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujie S, Aonuma H, Ito I, Gelperin A, Ito E: "The nitric oxide/cyclic GMP pathway in the olfactory processing system of the terrestrial slug Limax marginatus"Zool.Sci.. 19. 15-26 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chono K, Fujito Y, Ito E: "Non-ocular dermal photoreceotion in the pond snail Lymnaea stagnalis"Brain Res.. 951. 107-112 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi