• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The structural assemble and adaptive evolution of supramolecular hemoglobin

Research Project

Project/Area Number 14540629
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionThe University of Tokushima

Principal Investigator

GOTOH Toshio  The University of Tokushima, Faculty of Integrated Arts and Science, Professor (90035692)

Co-Investigator(Kenkyū-buntansha) OHASHI Makoto  The University of Tokushima, Faculty of Integrated Arts and Science, Professor (40128369)
MAKABE Kazuhiro  The University of Tokushima, Faculty of Integrated Arts and Science, Professor (60222288)
WATANABE Minoru  The University of Tokushima, Faculty of Integrated Arts and Science, Associate Professor (60338952)
SATOH Takanori  The University of Tokushima, Faculty of Integrated Arts and Science, Associate Professor (30276026)
松尾 義則  徳島大学, 総合科学部, 助教授 (80219401)
Project Period (FY) 2002 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Keywordshemoglobin / annelid / supramolecule / adaptation / polychaete
Research Abstract

Annelid extracellular hemoglobin is a supramolecule with molecular mass of ~3,500kDa. The giant hemoglobin consists of four types of total 144 globin chains and 36 linkers. In the present project, the amino acid sequences of globins a, A, b, and B of the giant hemoglobin from the polychaete Perinereis aibuhitensis have been determined by the combination of Edman degradation and RT-PCR method. The primary structures of Perinereis globin chains are compared with those of globins reported by others. From the point of view of structure and function of the giant annelid hemoglobins, we have noticed particularly at the cites of Cys residues in the amino acid sequences. There are 6 cites for the Cys residues in 32 sequences derived from the various giant hemoglobins. There are two Cys cites in the N-terminal region (cite 1, 2), two in the central region (cite 3, 4), one near the C-terminal region (cite 5), and one in the C-terminal region (cite 6). Cys residues are conserved in the cite 2 and 6. It has been also confirmed that Cys residues of the globins are all linked each other. Therefore, the Cys residues are all participated in the formation of SS bridges. The Cys sites of 32 sequences were categorized into 8 patterns: I (cite 2, 6), II (2, 4, 6), III (2, 4, 5, 6), IV (2, 5, 6), V (2, 3, 5, 6), VI (1, 2, 3, 5, 6), VII (1, 2, 5, 6), and VIII (1, 2, 6). The molecular phylogenetic tree of 32 globin sequences is divided into two branches of family A and B. The family A includes the types I, II, III, VI, VI of Cys distribution pattern, whereas the family B includes the types V, VI, VII, VIII. The relationship between the Cys distribution pattern and molecular adaptation has been discussed.

Report

(5 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (7 results)

All 2007 2005 Other

All Journal Article (5 results) Publications (2 results)

  • [Journal Article] Reassociation of annelid giant hemoglobin from the polychaete Perinereis aibuhitensis2007

    • Author(s)
      金井 拓
    • Journal Title

      自然科学研究, 徳島大学総合科学部 21

      Pages: 19-32

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Reass'ociation of axrnelid giant hemoglobin from thepolychaete Perinereis aibuhitensis2007

    • Author(s)
      Taku KANAI el al.
    • Journal Title

      Natural Science Reserch(Tokushima University) 21

      Pages: 19-32

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparative study on the primary structure of globin chains of the extracellular giant hemoglobins from the polychaete perinereis aibuhitensis and Tyulorrhynchus Heterochaetus2005

    • Author(s)
      山中 瑞恵
    • Journal Title

      自然科学研究, 徳島大学総台科学部 19

      Pages: 63-92

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparative study on the pnmary structure of globin chains of the extracellular giant hemoglobins from the polychaete pert nereis aibuhitensis and Tyulorrhynchus Helerochaelus2005

    • Author(s)
      Mizue YAMANAKA, et. al.
    • Journal Title

      Natural Science Reserch(Tokushlrna University) 19

      Pages: 63-92

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparative study on the primary structure of globin chains of the extracellur giant hemoglobins from the polychaete Perinereis aibuhitensis and Tylorrhynchus2005

    • Author(s)
      後藤寿夫
    • Journal Title

      徳島大学総合科学部 自然科学研究 19

      Pages: 63-92

    • Related Report
      2005 Annual Research Report
  • [Publications] 後藤寿夫: "環形動物巨大ヘモグロビンのグロビン鎖の共通名と分子系統樹"日本動物学会中国四国支部会報. 55. 6 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kanako Waki, Toshio Gotoh: "Amino acid sequences of the constituent chains of giant hemoglobin from the polypeptide Perinereis aibuhitensis and phylogenetic tree of annelid hemoglobins"Comparative Phyiosiology and Biochemistry. 134A, No.1. 223-223 (2003)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi