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Phylogenetic relationships and divergence times of reptiles inferred by mitochondrial DNA sequences

Research Project

Project/Area Number 14540641
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 系統・分類
Research InstitutionNagoya University

Principal Investigator

KUMAZAWA Yoshinori  Nagoya University, Graduate School of Science, Assistant Professor, 大学院・理学研究科, 講師 (60221941)

Co-Investigator(Kenkyū-buntansha) NISHIDA Mutsumi  University of Tokyo, Ocean Research Institute, Professor, 海洋研究所, 教授 (90136896)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordslizards / snakes / mitochondrial DNA / molecular phylogeny / divergence time / gene organization / vicariance / biogeography / 爬虫類 / PCR / 分子系統解析 / 高次系統分類 / 系統樹
Research Abstract

During the granted period, we have sequenced mitochondrial DNAs of various reptiles. These data have been characterized in both the evolution of mitochondrial genome organization and the pattern and timing of phylogenetic divergences. We found that mtDNAs of some reptile groups (e.g., snakes, varanids, agamids, and cordylids) have distinct gene organizations from the typical organization found in most vertebrates. We then addressed when and how these gene rearrangements took place on ancestral lineages of each group by comparing sequences of the corresponding mtDNA region among relevant taxa. With respect to phylogenetic matters, the following implications were obtained. 1) Lacertilia can be divided into four groups mostly corresponding to infraorders suggested by morphological studies, 2) Amphisbaenia is nested within Lacertilia and is the most closely related to Lacertidae, 3) Among the four lizard infraorders, Gekkota rather than Iguania diverged first, 4) Snakes are not closely related to Varanoidea, and 5) Lizard families have deep divergences extending to Triassic and Jurassic.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (27 results)

All 2005 2004 Other

All Journal Article (15 results) Book (2 results) Publications (10 results)

  • [Journal Article] Mitochondrial genome of Pogona vitticepes (Reptilia; Agamidae) : control region duplication and the origin of Australasian agamids.2005

    • Author(s)
      Amer, S.A.M., Kumazawa, Y.
    • Journal Title

      Gene 346

      Pages: 249-256

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Sister group relationship of turtles to the bird-crocodilian clade revealed by nuclear DNA-coded proteins.2005

    • Author(s)
      Iwabe, N., Hara, Y., Kumazawa, Y., Shibamoto, K., Saito, Y., Miyata, T., Kato, K.
    • Journal Title

      Molecular Biology and Evolution 22

      Pages: 810-813

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Complete mitochondrial DNA sequences of six snakes : phylogenetic relationships and molecular evolution of genomic features.2005

    • Author(s)
      Dong, S., Kumazawa, Y.
    • Journal Title

      Journal of Molecular Evolution 61

      Pages: 12-22

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Complete mitochondrial DNA sequences of six snakes : phylogenetic relationships and molecular evolution of genomic features.2005

    • Author(s)
      Dong, S., Kumazawa, Y.
    • Journal Title

      Journal of Molecular Evolution 61(1)

      Pages: 12-22

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Sister group relationship of turtles to the bird-crocodilian Glade revealed by nuclear DNA-coded proteins.2005

    • Author(s)
      Iwabe, N., Hara, Y., Kumazawa, Y., Shibamoto, K, Saito, Y, Miyata, T., Kato, K.
    • Journal Title

      Molecular Biology and Evolution 22(4)

      Pages: 810-813

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mitochondrial genome of Pogona vitticepes (Reptilia ; Agamidae) : control region duplication and the origin of Australasian agamids.2005

    • Author(s)
      Amer, S.A.M., Kumazawa, Y.
    • Journal Title

      Gene 346

      Pages: 249-256

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Sister group relationship of turtles to the bird-crocodilian clade revealed by nuclear DNA-coded proteins.2005

    • Author(s)
      Iwabe, N., Hara, Y., Kumazawa, Y., Shibamoto, K, Saito, Y, Miyata, T., Kato, K.
    • Journal Title

      Molecular Biology and Evolution (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The mitochondrial genome of Indonesian coelacanth Latimeria menadoensis (Coelacant hiformes) and divergence time estimation between the two coelacanths.2005

    • Author(s)
      Inoue, J.G., Miya, M., Venkatesh, B., Nishida, M.
    • Journal Title

      Gene (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Tempo of mitochondrial gene evolution : can mitochondrial DNA be used to date old divergences?2004

    • Author(s)
      Kumazawa Y., Azuma Y., Nishida M.
    • Journal Title

      Endocytobiosis and Cell Research 15

      Pages: 136-142

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Mitochondrial genome of the Komodo dragon : efficient sequencing method with reptile-oriented primers and novel gene rearrangements.2004

    • Author(s)
      Kumazawa Y., Endo, H.
    • Journal Title

      DNA Research 11

      Pages: 115-125

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mitochondrial DNA sequences of five squamates : phylogenetic affiliati on of snakes.2004

    • Author(s)
      Kumazawa Y.
    • Journal Title

      DNA Research 11

      Pages: 137-144

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mitochondrial genome of the Komodo dragon : efficient sequencing method with reptile-oriented primers and novel gene rearrangements.2004

    • Author(s)
      Kumazawa, Y., Endo, H.
    • Journal Title

      DNA Research 11(2)

      Pages: 115-125

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mitochondrial DNA sequences of five squamates : phylogenetic affiliation of snakes.2004

    • Author(s)
      Kumazawa, Y.
    • Journal Title

      DNA Research 11(2)

      Pages: 137-144

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mitochondrial genome of the Komodo dragon : efficient sequencing method with reptile-oriented primers and novel gene rearrangements.2004

    • Author(s)
      Kumazawa, Y., Endo, H.
    • Journal Title

      DNA Research 11

      Pages: 115-125

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mitochondrial DNA sequences of five squamates : phylogenetic affiliation of snakes.2004

    • Author(s)
      Kumazawa, Y.
    • Journal Title

      DNA Research 11

      Pages: 137-144

    • Related Report
      2004 Annual Research Report
  • [Book] 第7章 : 分子データを用いた魚類の分岐年代の推定(西田睦編)、海洋生命系のダイナミクス-第1巻、海洋の生命史

    • Author(s)
      熊澤慶伯
    • Publisher
      東海大学出版会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Dynamics of the Ocean Biosystems, vol.1. "Chapter 7 : Divergence time estimation for fishes using molecular data." (Mutsumi Nishida (ed))

    • Author(s)
      Kumazawa Y.
    • Publisher
      Tokai Univ. Press, Tokyo (in press)
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Kumazawa, Y., Endo, H.: "Mitochondrial genome of the Komodo dragon : efficient sequencing method with reptile-oriented primers and novel gene rearrangements"DNA Research. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kumazawa, Y.: "Mitochondrial DNA sequences of fire squamates : phylogenetic affiliation of snakes"DNA Research. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kumazawa, Y.et al.: "Tempo of mitochondrial gene evolution : can mitochondrial DNA be used to date old divergences?"Endocytobiosis and Cell Research. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miya, M.et al.: "Major patterns of higher teleostean phylogenies : a new perspective based on 100 complete mitochondrial DNA sequences"Molecular Phylogenetic and Evolution. 26. 121-138 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Inoue, J.et al.: "Evolution of the deep-sea gulper eel mitochondrial genomes : large scale gene rearrangements originated within the eels"Molecular Biology and Evolution. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kumazawa, Y. et al.: "Tempo of mitochondrial gene evolution : can mitochondrial DNA be used to date old divergences?"Endosymbiosis and Eukaryotlc organells. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Kumazawa, Y.: "Molecular clock estimation in fishes and its application to biogeographical studies"In i Proceedings of the International Commemorative Symposium for 70th Anniversaty of the Japanese Society of Fisheries Science. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] 西田睦: "脊椎動物の系統進化"月刊海洋. 号外29. 36-42 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Inoue, J.G.et al.: "Basal actinopteryglan relationships : a mitogenomic perspective on the phylogeny of the 'ancient fish'"Molecular Phy logenetics and Evolution. 26. 110-120 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Miya, M. et al.: "Major patterns of higher teleostean phylogenies : a new perspective based on 100 complete mitochondrial DNA sequences"Molecular Phy logenetics and Evolution. 26. 121-138 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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