| Project/Area Number |
14550770
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物・生体工学
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| Research Institution | Osaka University |
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Principal Investigator |
OMASA Takeshi Osaka University, Biotechnology, Assistant Professor, 大学院・工学研究科, 助手 (00252586)
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| Co-Investigator(Kenkyū-buntansha) |
OHTAKE Hisao Osaka University, Biotechnology, Professor, 大学院・工学研究科, 教授 (10127483)
KISHIMOTO Michimasa Osaka University, Biotechnology, Associate Professor, 大学院・工学研究科, 助教授 (00144436)
内山 進 大阪大学, 大学院・工学研究科, 助手 (90335381)
福井 希一 大阪大学, 大学院・工学研究科, 教授 (00311770)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | biologicals / Chinese hamster ovary cell / dihydrofolate reductase / methotrexate / chromosome targeting / gene amplification / gene amplification / dhfr / CHO / methotrexate / MTX |
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| Research Abstract |
Biologicals are important pharmaceutical products and recently the production is growing up. The most conventional method for biologicals production is using mammalian cell culture process. The most important points for production of biologicals using mammalian cell culture is the construction of mammalian cell line for production. Gene amplification is most conventional and complicated process for construction of productive mammalian cell. In this research, we investigated various types of MTX increasing methods using image analysis and obtained suitable gene amplification location of chromosome "P". We constructed suitable host cell line for gene amplification in location "P" and the objective gene was inserted in this location by recombinase reaction.
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