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Development of the multi-ion-imaging sensor and its application to biological specimens

Research Project

Project/Area Number 14550791
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 工業物理化学
Research InstitutionOsaka University

Principal Investigator

YOSHINOBU Tatsuo  Osaka University, The Institute of Scientific and Industrial Research, Associate Professor, 産業科学研究所, 助教授 (30243265)

Co-Investigator(Kenkyū-buntansha) IWASAKI Hiroshi  Osaka University, The Institute of Scientific and Industrial Research, Professor, 産業科学研究所, 教授 (00029901)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordssensor / chemical sensor / ion / semiconductor device / LAPS / chemical imaging sensor
Research Abstract

1. Development of multi-ion-imaging technology
The conditions for the fabrication of ion-sensitive membranes with ionophores were optimized to implement various ion sensitivities on the sensing surface of the light-addressable potentiometric sensor (LAPS). A photo-patterning process was developed for the integration of ion-sensitive photocurable membranes on a single sensor chip. The distribution of the ion concentration was visualized with a sensor with integrated K^+-and Ca^<2+>-sensitive membranes.
2. Development of Enzyme LAPS
For the application of the LAPS and the chemical imaging sensor to biological specimens, enzyme LAPS was developed with urease and butyrylcholinesterase. Various methods for the immobilization of enzyme molecules on the sensor surface were compared in terms of sensitivity and stability.
3. Development of a pen-shaped portable LAPS device
To allow more flexible use of LAPS; a pen-shaped portable LAPS device was developed. The control and measurement circuits are built in the device, which can be directly dipped into the solution to be measured. The sensor chip has 4 or 16 measurement points on the surface, each of which can be independently assigned for measurement of different ion species.
4. Application to the microfluidic devices
For the application to the detection and measurement of chemical species in the microfluidic devices such as μTAS and Lab-on-a-Chip, a LAPS-based system was combined with a microfluidic channels fabricated with the SU-8/PDMS technology. This system allows label-free measurement of ion concentration at an arbitrary point in the microchannel. The distribution of the ion concentration in the microchannel was visualized.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Yu.Ermolenko et al.: "The Double K^+/Ca^<2+> Sensor Based on Laser Scanned Silicon Transducer (LSST) for Multi-Component Analysis"Talanta. 59・4. 785-795 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yu.G.Mourzina et al.: "Anion-Selective Light-Addressable Potentiometric Sensors (LAPS) for the Determination of Nitrate and Sulphate Ions"Sensors and Actuators B. 91・1-3. 32-38 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yu.Mourzina et al.: "K^+-Selective Field-Effect Sensors as Transducers for Bioelectronic Applications"Electrochimica Acta. 48・20-22. 3333-3339 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yoshinobu et al.: "AFM Fabrication of Oxide Patterns and Immobilization of Biomolecules on Si Surface"Electrochimica Acta. 48・20-22. 3131-3135 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Kodera et al.: "Detection of Protein-Protein Interactions on SiO_2/Si Surfaces by Spectroscopic Ellipsometry"Analytical Biochemistry. 321・1. 65-70 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.B.Md.Ismail et al.: "Investigation on Light-Addressable Potentiometric Sensor as a Ppossible Cell-Semiconductor Hybrid"Biosensors and Bioelectronics. 18・12. 1509-1514 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yoshinobu et al.: "Portable Light-Addressable Potentiometric Sensor (LAPS) for Multisensor Appications"Sensors and Actuators B. 95・1-3. 352-356 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] I.G.Mourzina et al.: "Immobilization of Urease and Cholinesterase on the Surface of Semiconductor Transducer for the Development of Light-Addressable Potentiometric Sensors"Microchimica Acta. 144・1-3. 41-50 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yu.Ermolenko et al.: "The Double K^+/Ca^<2+> Sensor Based on Laser Scanned Silicon Transducer (LSST) for Multi-Component Analysis"Talanta. 59(4). 785-795 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yu.G.Mourzina et al.: "Anion-Selective Light-Addressable Potentiometric Sensors (LAPS) for the Determination of Nitrate and Sulphate Ions"Sensors and Actuators B. 91(1-3). 32-38 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Iwasaki et al.: "K^+-Selective Field-Effect Sensors as Transducers for Bioelectronic Applications"Electrochimica Acta. 48(20-22). 3333-3339 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yoshinobu et al.: "AFM Fabrication of Oxide Patterns and Immobilization of Biomolecules on Si Surface"Electrochimica Acta. 48(20-22). 3131-3135 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Kodera et al.: "Detection of Protein-Protein Interactions on Si0_2/Si Surfaces by Spectroscopic Ellipsometry"Analytical Biochemistry. 321(1). 65-70 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.B.Md.Ismail et al.: "Investigation on Light-Addressable Potentiometric Sensor as a Possible Cell-Semiconductor Hybrid"Biosensors and Bioelectronics. 18(12). 1509-1514 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yoshinobu et al.: "Portable Light-Addressable Potentiometric Sensor (LAPS) for Multisensor Applications"Sensors and Actuators B. 95(1-3). 352-356 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] I.G.Mourzina et al.: "Immobilization of Urease and Cholinesterase on the Surface of Semiconductor Transducer for the Development of Light-Addressable Potentiometric Sensors"Microchimica Acta. 144(1-3). 41-50 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.B.Md.Ismail et al.: "Investigation on Light-Addressable Potentiometric Sensor as a Possible Cell-Semiconductor Hybrid"Biosensors and Bioelectronics. Vol.18,no.12. 1509-1514 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Yoshinobu et al.: "AFM Fabrication of Oxide Patterns and Immobilization of Biomolecules on Si Surface"Electrochimica Acta. vol.48,nos.20-22. 3131-3135 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yu.Mourzina et al.: "K^+-Selective Field-Effect Sensors as Transducers for Bioelectronic Applications"Electrochimica Acta. vol.48,nos.20-22. 3333-3339 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Yoshinobu et al.: "Portable Light-Addressable Potentiometric Sensor (LAPS) for Multisensor Applications"Sensors and Actuators B. vol.95,nos.1-2. 352-356 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Poghossian et al.: "Flow-Velocity Microsensors Based on Semiconductor Field-Effect Structures"Sensors. vol.3. 202-212 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Kodera et al.: "Detection of Protein-Protein Interactions on SiO_2/Si Surfaces by Spectroscopic Ellipsometry"Analytical Biochemistry. vol.321,no.1. 65-70 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yu.Ermolenko et al.: "The Double K^+/Ca^<2+> Sensor Based on Laser Scanned Silicon Transducer (LSST) for Multi-Component Analysis"Talanta. 59・4. 785-795 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yu.G.Mourzina et al.: "Anion-Selective Light-Addressable Potentiometric Sensors (LAPS) for the Determination of Nitrate and Sulphate Ions"Sensors and Actuators B. (in press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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