| Project/Area Number |
14560019
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | University of Tsukuba |
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Principal Investigator |
HANDA Takashi University of Tsukuba, Graduate School of Life and Environmental Sciences, Associate professor (00192708)
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| Co-Investigator(Kenkyū-buntansha) |
YUKAWA Tomohisa 独立行政法人国立科学博物館, National museum of nature and science, Tsukuba botanical garden, Senior researcher (50280524)
倉重 祐二 新潟県立植物園, 研究官
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| Project Period (FY) |
2002 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | azalea / cultivar / DNA / AFLP / IGS |
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| Research Abstract |
Within about 50 Rhododendron species native to Japan, subgenus Tsutsusi section Tsutsusi is the most important genetic resource for ornamental evergreen azaleas. However, their origin and parental species are still unknown. In addition, the genus has posed systematic problems in terms of infrageneric circumscription and ranks. Molecular phylogenetic analysis based on DNA sequence such as ITS and matKregions haven't been clarified the relationships within the subgenus Tsutsusi due to the close relationship between species (Kurashige et al. 2001*).
In this study, to clarify the origin of Japanese ornamental evergreen azaleas, we investigated phylogenetic relationships among almost all subgenus Tsutsusi species (20 taxa) native to Japan by AFLP markers.
DNA isolated from 97 plants of 20 species and varieties of subgenus Tsutsusi were subjected to AFLP analysis. R. tashiroi (subgenus Sciadorhodion) was used as outgroup species.
For all plants, 2 primer sets (EcoRI-ACT/MseI-CTA, EcoRI-AAG/MseI-CAT) were used. For 22 plants of subsect. Tsutsusi and Macrosepala, 4 primer sets (EcoRI-ACT/MseI-CAT, EcoRI-AAG/MaeI-CTA) were used.
Phylogenetic trees were calculated by the neighbor-joining (NJ) method of the PAUP program. The bootstrap method on 1,000 replicated was assessed.
AFLP analysis can provide useful information of the phylogenetic relationship of subgenus Tsutsusi. AFLP markers specific to each taxon or population will be applicable to clarify the origin of Japanese evergreen azalea cultivars in future.
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