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Analyses and application of self-incompatibility gene products in the Japanese pear

Research Project

Project/Area Number 14560021
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionMie University

Principal Investigator

HIRATSUKA Shin  Mie Univ, Fac. of Bioresources, Professor, 生物資源学部, 助教授 (10143265)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsJapanese pear / self-incompatibility / S-RNase / S-gene expression / cultivar difference / self-thinning cultivar / S遺伝子 / 花粉管伸長 / 受精 / 着果
Research Abstract

In pollen tubes treated with S-RNase in vitro, phosphoenolpyruvate carboxylase (PEPC) and isocitrate dehydrogenase (ICDH) activities showed little reduction, but invertase (INV) and glutaraldehyde 3-phosphate dehydrogenase (GPD) were strongly inactivated. PEPC and ICDH may be present as a protein form in a pollen grain, whereas INV and GPD may be newly synthesized during pollen-tube development. The enzymes involved in energy metabolism such as INV and GPD may be targets of S-RNase.
Cultivar difference in S4-RNase distribution in the style was investigated using the S4-RNase atiserum. 'Kosui' contained higher S4-RNase in upper and middle part of the style, but 'Nijisseiki' did in middle part. The S4-RNase was clearly detected immunologically in 'Osa-Nijisseiki' style and almost all the RNase contained in upper style of the cultivar.
S-RNase gene expression was cultivar dependent; lower expression of S2-RNase was observed in 'Gold-Niisseiki' and 'Osa-Nijisseiki'. S4-RNase expression was lower in 'Kosui', 'Osa-Nijisseiki', and so on. Clear relationships, however, were not observed between the gene expression and S-RNase content.
S2-RNase gene expression declined by 20% of control (the day of anthesis) two days after pollination; pollination type (cross-, self-, or non-pollination) seemed not to affect the expression level. However, the level one day after pollination showed following order; cross>non>self. The style may recognize its mate and regulate S-RNase expression during early stages of pollen-tube elongation.
Fruit set was 19.4% following self-pollination in weakly self-incompatible 'Okusankichi', whereas no fruit was obtained in strongly self-incompatible 'Hakko'. Several individuals among the F1 progenies between both cultivars showed a self fruitful character, indicating that weak incompatibility of 'Okusankichi' is heritable. Thus, breeding of self-thinning cultivar will be successful in the near future.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Zhang, S-L., S.Hiratsuka: "Analyses of pollen-tube growth and biological action of S-RNase in the self-compatible Japanese pear."Sci.Hort.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiratsuka, S.et al.: "Self-incompatibility in Japanese pears : Peculiar inhibitory action of S-RNase on self-pollen tube growth in vitro"Acta Hort.. 636. 347-356 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiratsuka, S.et al.: "Flower thinning by calcium formate in Japanese pears : concentration effects and its uptake into styles."J.Japan.Soc.Hort.Sci.. 72. 224-229 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiratsuka, S., S-L.Zhang: "Relationships between fruit set, pollen-tube growth, and S-RNase concentration in the self-incompatible Japanese pear."Sci.Hort.. 96. 309-318 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiratsuka, S., S-L.Zhang: "Cultivar differences in the expression of self-incompatibility in Japanese pears."Acta.Hort.. 587. 437-448 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiratsuka, S.et al.: "Action mode of calcium formate as a chemical thinner on Japanese pear flower."J.Japan.Soc.Hort.Sci.. 71. 62-67 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 平塚 伸 他: "新農学大辞典"養賢堂 (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zhang, S-L, S. Hiratsuka: "Analyses of pollen-tube growth and biological action of S-RNase in the self-compatible Japanese pear."Sci. Hort. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiratsuka, S. et al.: "Self-incompatibility in Japanese pears; Peculiar inhibitory action of S-RNase on self pollen-tube growth in vitro."Acta Hort. 636. 347-356 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiratsuka, S. et al.: "Flower thinning by calcium formate in Japanese pears; Concentration effects and its uptake into styles. (in Japanese)."J. Japan. Soc. Hort. Sci. 72. 224-229 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiratsuka, S, S-L. Zhang: "Relationships between fruit set, pollen-tube growth, and S-RNase concentration in the self-incompatible Japanese pear."Sci. Hort. 96. 309-318 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiratsuka, S, S-L. Zhang: "Cultivar differences in the expression of self-incompatibility in Japanese pears."Acta Hort. 587. 437-448 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiratsuka, S. et al.: "Action mode of calcium formate as a chemical thinner on Japanese pear flower. (in Japanese)."J. Japan. Soc. Hort. Sci. 71. 62-67 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S-L.Zhang, S.Hiratsuka: "Analyses of pollen-tube growth and biological action of S-RNase in the style of self-incompatible Japanese pear"Sci.Hort.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Hiratsuka et al.: "Self-incompatibility in Japanese pears : Peculiar inhibitory action of S-RNase on self pollen-tube growth in vitro"Acta Hort.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 平塚伸 他: "ギ酸カルシウムによるニホンナシの摘花:濃度の影響と花桂による薬剤の吸収"園学雑. 72. 224-229 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Hiratsuka, S-L.Zhang: "Relationships between fruit set, pollen-tube growth, and S-RNase concentration in the self-incompatible Japanese pear"Sci.Hort.. 95. 309-318 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Hiratsuka, S-L.Zhang: "Cultivar differences in the expression of self-incompatibility in Japanese pears"Acta Hort.. 587. 437-448 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 平塚伸 他: "ニホンナシに対するギ酸カルシウムの摘花作用"園学雑. 71. 62-67 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 平尾香那子 他: "ニホンナシの自家不和合性に関する研究(第37報)自家和合性ニホンナシ'おさ二十世紀'のS_4-RNaseに関する免疫学的研究"園学雑. 72(別2). 329 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 平塚伸 他: "ニホンナシの自家不和合性に関する研究(第38報)'晩三吉'の自家結実性は後代に遺伝するか?"園学雑. 72(別2). 330 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Hiratsuka et al.: "RNaes T_1,T_2 and A can not substitute for S-RNase in growth inhibition and RNA degradation of in vitro-grown pear pollen"Abstr.Int.Symp.plant Self-Incompatibility. 32 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Hiratsuka et al.: "Self-incompatibility in Japanese pears : Peculiar inhibitory action of S-RNaes on self pollen-tube growth in vitro"Abstr.XXVI^th Int.Hort.Congr.. 359-360 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 平野豊士 他: "ニホンナシの自家不和合性に関する研究(第36報)培地へのS-RNase添加が不和合花粉のPEPC活性に及ぼす影響"園学雑. 71(別2). 491 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 平塚 伸: "新農学大辞典:果樹の栽培〜結実生理(自家不和合性等)〜"養賢堂(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Hiratsuka, et al.: "Self-incompatibility in Japanese pears : Peculiar inhibitory action of S-RNase on self pollen-tube growth in vitro"Acta Hort.. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Hiratsuka, S-L.Zhang: "Relationships between fruit set, pollen-tube growth, and S-RNase concentration in the self-incompatible Japanese pear"Sci. Hort.. 95. 309-318 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Hiratsuka, S-L.Zhang: "Cultivar differences in the expression of self-incompatibility in Japanese pears"Acta Hort.. 587. 437-448 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Hiratsuka, et al.: "Self-incompatibility in Japanese pears : Peculiar inhibitory action of S-RNase on self pollen-tube growth in vitro"Abstr. XXVlth Internat. Hort. Congr.. 359-360 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 平野豊士, 河合義隆, 平塚伸: "ニホンナシの自家不和合性に関する研究(第36報)培地へのS-RNase添加が不和合花粉のPEPC活性に及ぼす影響"園学雑. 71(別2). 491 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 平塚 伸: "新農学大辞典:果樹の栽培〜結実生理(自家不和合性等)〜"養賢堂(印刷中).

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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