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Analysis of the mechanism of new flower color on trnsgenic horticultural plant.

Research Project

Project/Area Number 14560033
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionMinami Kyusyu University

Principal Investigator

YAMAGUCHI Masa-atsu  Minamikyushu University, Faculty of Horticulture, Professor (10125180)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsDianthus caryophyllus / Caryopyllaceae / Transgenic plant / Anthocyanin / Flavonoid 3', 5'-hydroxylase / blue carnation / Flavonol / Biosynthetic pathway / 花色 / 生合性 / フラボン / ケンフェロール / ミリセチン / F3'5'H / 遺伝子組換え / フラボノイド3',5'ヒドロキシラーゼ / フラボノイド生合成 / 青色育種 / 花色素
Research Abstract

The object of the present study was to analysis of the mechanism of flower color expression in trasgenic carnation cultivars expressing the flavonoid 3', 5'-hydroxylase (F3', 5'H) based on the anthocyanin biosynthetic pathway. Flower color pigment and flavonoid component analyses were carried out for blue carnaton cultivars using some chromatography. We conclued that in the transgenic carnation, delphynidin type anthocyanins are contained as a result of F3', 5'H gene expression, but delphynidin related new flavonoid component such as myricetin are not contained. In a trasgenic carnation cultivar, some germinated pollen was observed.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report

Research Products

(14 results)

All 2004 2003 Other

All Journal Article (8 results) (of which Peer Reviewed: 2 results) Publications (6 results)

  • [Journal Article] Variation in chalcononarigenin 2'-0-glucoside content in the petals of carnation(Dianthus caryophyllus).2004

    • Author(s)
      Yoshida,H., Itoh,Y., Ozeki,Y., Iwashina,T., Yamaguchi,M.
    • Journal Title

      Euphytica 99

      Pages: 175-186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Identification of a cDNA encoding malonyl-CoA : antho-cyanidin 3-0-glucoside 6"-0-malonyltransferase from cineraria(Senecio cruentas)2004

    • Author(s)
      Suzuki,H., Sawada,S., Sakakibara,K., Nakayama,T, Yamaguchi,M.
    • Journal Title

      Plant Biotechnology 135

      Pages: 247-253

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Variation in chalcononarigenin 2'-O-glucoside content in the petals of carnation(Dianthus caryophyllus)2004

    • Author(s)
      Yoshida, H., Itoh, Y., Ozeki, Y., Iwashina, T., Yamaguchi, M.
    • Journal Title

      Euphytica 99

      Pages: 175-186

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Identification of a cDNA encoding malonyl-CoA : antho-cyanidin 3-O-glucoside 6"-O-malonyltransferase from cineraria(Senecio cruentas)2004

    • Author(s)
      Suzuki, H., Sawada, S., Sakakibara, K., Nakayama, T, Yamaguchi, M.
    • Journal Title

      Plant Biotechnology 135

      Pages: 247-253

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Dianthus属の黄色花に関する研究2003

    • Author(s)
      吉田洋之・伊藤佳央・小関良宏・山口雅篤
    • Journal Title

      園芸学会雑誌別冊 72(1)

      Pages: 297-297

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Dianthus属の花色発現機構に関する研究2003

    • Author(s)
      吉田洋之・鈴木浩二・長江嗣朗・山口雅篤
    • Journal Title

      園芸学会雑誌別冊 72(1)

      Pages: 130-130

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Studies on the mechanism of flower color expression in genus Dianthus2003

    • Author(s)
      Yoshida, H., Suzuki, Y., Nagae, Y., Yamaguchi, M.
    • Journal Title

      J.Japan, Soc.Hort.Sci. 72(1)

      Pages: 130-130

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Studies on the mechanism of yellowflower color in genus Dianthus2003

    • Author(s)
      Yoshida, H., Suzuki, Y., Ozeki, Y., Yamaguchi
    • Journal Title

      J.Japan, Soc.Hort.Sci. 72(1)

      Pages: 297-297

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshida, H., Y., Itoh, Y., Ozeki, T., Iwashina, M., Yamaguchi: "Variation in chalcononarigenin 2'-O-glecoside content in the petals of carnations (Dianthus caryophyllus) bearing yellow flowers"Euphytica. 99. 175-186 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki, H., S., Sawada, K., Sakakibara, T., Nakayama, M., Yamaguchi: "Identification of a cDNA encoding malony-coenzyme A : anthocyanidin 3-O-glucoside 6"-O-malonyltraqnsferase from cineraria (Senecio cruentus)"Plant Biotechnology. 135. 247-253 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 吉田洋之, 鈴木浩二, 長江嗣朗, 山口雅篤: "Dianthus属の花色発現機構に関する研究(第1報)脱アシル化アントシアニンによる花色変異"園芸学会雑誌. 72(1). 130 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 吉田洋之, 伊藤佳央, 小関良宏, 山口雅篤: "Dianthus属の黄色花に関する研究(第5報)ダイアンサス黄色花における黄色形質の発現機構"園芸学会雑誌. 72(1). 297 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 吉田洋之, 鈴木浩二, 長江嗣郎, 山口雅篤: "Dianthus属の花色発現機構に関する研究"園芸学雑誌別冊1号. 72. 130 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 吉田洋之, 伊藤佳央, 小関良宏, 山口雅篤: "Dianthus属の黄色花に関する研究"園芸学雑誌別冊1号. 72. 297 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-03-31   Modified: 2016-04-21  

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