| Project/Area Number |
14560038
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
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| Research Institution | Kyoto University |
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Principal Investigator |
YAMASUE Yuji Kyoto University, Graduate School of Agriculture, Associate Professor, 農学研究科, 助教授 (60093332)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAZAKI Tetsuya Kyoto University, Graduate School of Agriculture, Instructor, 農学研究科, 助手 (60217693)
OKUMOTO Yutaka Kyoto University, Graduate School of Agriculture, Associate Professor, 農学研究科, 助教授 (90152438)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Herbicide resistance / Tainu-bie / Echinochica oryzicola / Echinochica phyllopogon / Echinochica weeds / P-450 / Herbicide resisitance / Echinochola oryzicola / Echinochola phyllopogon |
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| Research Abstract |
In 1997, a multiple-herbicide resistant biotype of E.oryzicola, was found at the rice paddy area of Sacramento in California. This resistant biotype was studied with objectives to elucidate (1) its relative fitness against susceptible biotype and (2) mechanism of the resistance.
1.Relative fitness of the resistant biotype
The number of seeds produced by a plant was measured as a criterion of fitness on individuals grown in a transplanted field experiment in Kyoto and on those grown at various fields of Sacramento. The relative fitness of resistant biotype ranged from 0.70 to 0.73 in the transplanted experiment of Kyoto and from 0.50 to 0.58 in the actual field survey at Sacramento.
2.Mechanism of the resistance
Mechanism of the resistant biotype to several herbicides that differ in chemical structure and mode of action was studied with an attention to the microsomal P-450. In biochemical and molecular analyses of this project, it was found that the resistant biotype induced larger P-450 activity than the susceptible biotype when treated with a herbicide, and that more than a species of P-450s were involved in the herbicide resistance.
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