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Ecological studies on the bacterial rotting diseases of Siitake mushroom

Research Project

Project/Area Number 14560044
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionTokyo University of Agriculture

Principal Investigator

SUYAMA K.  Tokyo University of Agriculture, Fac.of Agriculture, Professor, 農学部, 教授 (10078181)

Co-Investigator(Kenkyū-buntansha) 根岸 寛光  東京農業大学, 農学部, 助教授 (20172752)
SAWAYANAGI T.  Tokyo University of Agriculture, Fac.Agriculture, Associate researcher, 農学部, 助手 (90372987)
有馬 忍  大分県きのこ研究指導センター, 主査
村上 康明  大分県きのこ研究指導センター, 主幹研究員
NEGISHI H.  Tokyo University of Agriculture, Fac.of Agriculture, Associate professor
ARIMA S.  Ooita mushroom research institute, Researcher
MURAKAMI Y.  Ooita mushroom research institute, Researcher
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsSiitake mushroom / Bacterial diseases / Pseudomonas tolaasii / Erwinia sp. / Ecology / Wild mushroom / Dissemination by insects / WLRO / 簡易検定法の開発 / T-PAF培地 / きのこ / 細菌病 / ハラアカコブカミキリ / ナカモンミナミコノコバエ
Research Abstract

Ecological studies of bacterial blotch and rot diseases occurred on Siitake mushroom were carried during 2002-2004 and obtained following results.
1.Pathogens of bacterial blotch and rot disease were detected from many rotting fruit bodies collected from all over the cultivation places in Ooita Prefecture. Erwinia sp.caused bacterial rot disease was mainly isolated and Pseudomonas tolaasii caused bacterial blotch disease was isolated lower frequency from rotting fruit bodies of Siitake mushrooms. This results suggested that causal pathogens were widely distribute over the cultivation area and given sever damages on the Siitake mushrooms in Ooita Prefecture.
2.Improvement of selective medium, T-PAF and development of new diagnosis method for Pseudomonas tolaasii using PCR reaction were examined. T-PAF added microtol D, cleaning chemical used for materials cultured mushrooms, suppressed the growth of microorganisms living in cultured houses of mushrooms except the growth of P.tolaasii. Addition of microtol D was utility for selection of P.tolaasii, however it lost white line formation activity which was benefit tool for distinction of P.tolaasii. PCR reaction used a DNA fragment construct from tolasin gene of P.tolaasii was useful for simple identification method with rapidly and exactly.
3.Attempt to detect the causal agents of rotting fruit bodies of Siitake from cultivation environment was made. P.tolaasii and Erwinia sp.could isolated from twigs used as artificial shade of logs, logs, soil of planting and a kind of flies attacked fruit bodies. We newly found that wild mushrooms collected from mountains area infected with P.tolaasii. From these results, we considered that soil of planting and cultured Siitake mushroom was important sources of causal agents and some of flies would have a role of dissemination of casual agents. The role of wild mushrooms infected with P.tolaasii was not known.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (6 results)

All 2004 2002 Other

All Journal Article (4 results) Publications (2 results)

  • [Journal Article] 大分県におけるシイタケ腐敗症の発生状況について2004

    • Author(s)
      有馬忍, 陶山一雄
    • Journal Title

      九州森林研究 57

      Pages: 275-276

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Occurrence of brown rot of Siitake mushroom in Oita Prefecture.2004

    • Author(s)
      ARIMA S., SUYAMA K.
    • Journal Title

      Kyushu J.of Forest Research 57

      Pages: 275-276

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] きのこ腐敗病菌Pseudomonas tolaasiiの選択T-PAF培地の改良2002

    • Author(s)
      角田茂幸, 陶山一雄
    • Journal Title

      日本応用きのこ学会 講演要旨集

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Improvement of T-PAF, a selective media for isolation of Pseudomonas tolaasii.2002

    • Author(s)
      TUNODA S., SUYAMA K.
    • Journal Title

      Mushroom Science and Biotechnology ; Annual Report (Abstract)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 有馬忍, 陶山一雄: "大分県におけるシイタケ腐敗病の発生状況について"九州森林研究. 57巻. 275-276 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 角田茂幸, 陶山一雄: "きのこ腐敗病菌Pseudomonas tolaasii選択培地の改良"日本応用きのこ学会大会. 67 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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