Global analysis of symbiotic tissue specific gene expressions of nitrogen and carbon metabolisms in legume nodules

• Project/Area Number: 14560054
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Kagawa University
• Principal Investigator: TAJIMA Shigeyuki Kagawa University, Department of Life Science, Professor, 農学部, 教授 (50116894)
• Co-Investigator(Kenkyū-buntansha): AGARIE Mika (NOMURA Mika) Kagawa University, Department of Life Science, Associate Professor, 農学部, 助教授 (50294749)
• Project Period (FY): 2002 – 2003
• Project Status: Completed (Fiscal Year 2003)
• Budget Amount: ¥3,700,000 (Direct Cost: ¥3,700,000); Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2002: ¥2,600,000 (Direct Cost: ¥2,600,000)
• Keywords: Symbiotic Nitrogen Fixation / Macroarray / Proteome / RNAi / Transgenic plants / Lotus Japonicus / SNARE / Antisense / ダイズ / マメ科植物 / 根粒 / ミトコンドリア / PMF(peptide mass fingerprint)解析 / 自動エドマン法

Research Abstract

1)Proteome analysis of nodule mitochondria and nodule bacteroids of soybean plants was surveyed, In. the case of nodule mitochondria 2D-EP following Protein Mass Finger print and LCMSMS analysis revealed that 465 spots (nodule mitochondria) and 383 spots (root mitochondria) were tissue specific. Using Edman degradation methods, Protein Mass Finger print and LCMSMS analysis identification of these protein spots were performed. Although there are many spots which the function were not registered in the database, the data showed 7 up-regulating and 8 down-regulating proteins were identified. Six suppressed proteins in nodule mitochondria were identified too. The role in nodule metabolism of these proteins were discussed. Similar approach was employed in the case of nodule bacteroids too. In this bacteroid proteome experiment, we identified 62 proteins which were up-regulated in nodule bacteroids comparing to cultured bacteria.
2)For preparing genome sequencing data we joined to the project team of Kazusa DNA Research Institute and Dr. Kouchi' laboratory. In this experiment we sequenced more than 9,000 cDNA clones from 5'site of the clone and the data is going to supplied to the public through Kazusa DNA Research Institute.

Research Products

• [Publications] Uchiumi T, Ohwada T, Itakura M, Mitsui H, Nukui N, Dawadi P, Kaneko T, Tabata S, Yakoyama T, Tejima K, Saeki K, Omori H, Hayashi M, Maekawa T, Sriprang R, Murooka Y, Tajima S, Simomura K, Nomura M, Suzuki A, Shimoda Y, Sioya K, Abe M, Minamisawa K.: "Expression islands clustered on the symbiosis island of the Mesorhizobium loti genome."J.Bacteriology. 186. 2439-2448 (2004)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hoa, L., Mika Nomura, Shigeyuki Tajima: "Characterization of bacteroid proteins in soybean nodules formed with Bradyrhizobium japonicum USDA110."Microbes & Environment. 19. 71-75 (2004)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hoa, L., Mika Nomura, Hideyuki Kajiwara, David Alexander Day, Shigeyuki Tajima: "Proteomic analysis on symbiotic differentiation of mitochondria in soybean nodules."Plant Cell Physiol. 45. 300-308 (2004)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Uchiumi T, Ohwada T, Itakura M, Mitsui H, Nukui N, Dawadi P, Kaneko T, Tabata S, Yokoyama T, Tejima K, Saeki K, Omori H, Hayashi M, Maekawa T, Sriprang R, Murooka Y, Tajima S, Simomura K, Nomura M, Suzuki A, Shimoda Y, Sioya K, Abe M: "Minamisaw, Expression islands clustered on the symbiosis island of the Mesorhizobium loti genome."J.Bacteriology. 186. 2439-2448 (2004)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hoa, L., Mika Nomura, Shigeyuki Tajima: "Characterization of bacteroid proteins in soybean nodules formed with Bradyrhizobium japonicum USDA11O."Microbes and Environment. 19. 71-75 (2004)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hoa, L., Mika Nomura, Hideyuki Kajiwara, David Alexander Day, Shigeyuki Tajima: "Proteomic analysis on symbiotic differentiation of mitochondria in soybean nodules."Plant Cell Physiology. 45. 300-308 (2004)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Uchiumi T, Ohwada T, Itakura M, Mitsui H, Nukui N, Dawadi P, Kaneko T, Tabata S, Yokoyama T, Tejima K, Saeki K, Omori H, Hayashi M, Maekawa T, Sriprang R, Murooka Y, Tajima S, Simomura K, Nomura M, Suzuki A, Shimoda Y, Sioya K, Abe M, Minamisawa: "Expression islands clustered on the symbiosis island of the Mesorhizobium loti genome"J. Bacteriology. 186.8. 2439-2448 (2004)
• [Publications] Hoa, L., Mika Nomura, Shigeyuki Tajima: "Characterization of bacteroid proteins in soybean nodules formed with Bradyrhizobium japonicum USDA110"Microbes & Environment. (In press). (2004)
• [Publications] Hoa, L., Mika Nomura, Hideyuki Kajiwara, David Alexander Day, Shigeyuki Tajima: "Proteomic analysis on symbiotic differentiation of mitochondria in soybean nodules"Plant Cell Physiol. 45. 300-308 (2004)
• [Publications] Sinsuwongwat, S., Kodera, A., Kaneko, T., Tabata, S., Nomura, M.Tajima, S.: "Cloning and characterization of a NADP+malic enzyme gene from Bradyhizobiuzm japonicum USDA110"Soil Science and Plant Nutr.. 48,5. 711-717 (2002)
• [Publications] Sinsuwongwat, S., Nuntagij, A., Shutsrirung, A., Nomura, M.Tajima, S.: "Characterization of local Rhizobia in Thailand and the distribution of malic enzyme"Soil Science and Plant Nutr.. 48,5. 719-727 (2002)