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Molecular Characterization of Reversible Decarboxylases Catalyzing CO_2 Fixation and Their Application to Molecular Conversion Process

Research Project

Project/Area Number 14560061
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionGifu University

Principal Investigator

YOSHIDA Toyokazu  Gifu University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (90220657)

Co-Investigator(Kenkyū-buntansha) MITSUKURA Koichi  Gifu University, Faculty of Engineering, Research Assistant, 工学部, 助手 (70324283)
NAGASAWA Toru  Gifu University, Faculty of Engineering, Professor, 工学部, 教授 (60115904)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsDecarboxylase / Carbon dioxide fixation / Aromatic carboxylic acids / Cloning / Microbial conversion / 炭酸固定 / 4-ヒドロキシ安息香酸 / レゾルシン酸 / ピロール-2-カルボン酸 / インドール-3-カルボン酸 / ピロール / インドール
Research Abstract

Generally, decarboxylases can not catalyze the reverse carboxylation reaction. However, we found novel decarboxylases efficiently catalyzing nonoxidative decarboxyation. In the metabolism of phenol in the absence of oxygen, 4-hydroxybenzoate decarboxylase found to catalyze carbon dioxide fixation. In the view point of application to chemical industry, the reversible decarboxylases are useful for the synthesis of aromatic carboxylic acids, because a carboxyl group is regioselectively introduced into aromatic ring. In the present research, we screened novel decarboxylases reversibly catalyzing nonoxidative decarboxylation, revealed physicochemical and molecular characteristics, and evaluated the reverse carboxylation activities.
The primary structures of pyrrole-2-carboxylate decarboxylase and indole-3carboxylate decarboxylase were determined from their gene analysis. These decarboxylases showed similarity to various microbial hypothetical proteins. In the screening of 4-hydroxybenzoate decarboxylase, the enzyme activity was widely detected in various bacteria, actinomycetes and yeasts, contrary to the previous observation. As for 2,6-dihydroxybenzoate decarboxylase, a novel decarboxylase, the efficient regioselecetive carboxylation of 1,3-dihydroxybenzene was confirmed in the presence of KHCO_3. From the analyses for enzymatic properties and structures, various reversible decarboxylase were classified into two subgroups.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Toyokazu Yoshida, Kohei Fujita, Toru Nagasawa: "Novel Reversible Indole-3-carboxylate Decarboxylase Catalyzing Nonoxidative Decarboxylation"Bioscience, Biotechnology and Biochemistry. 66. 2388-2394 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Toyokazu Yoshida, Yutaka Hayakawa, Tsuyoshi Matsui, Toru Nagasawa: "Purification and Characterization of 2,6-Dihydroxybenzoate Decarboxylase Catalyzing Nonoxidative Decarboxiation"Archives of Microbiologu. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Toyokazu Yoshida, Kohei Fujita, Toru Nagasawa: "Novel Reversible Indole-3-carboxylate Decarboxylase Catalyzing Nonoxidative Decarboxylation"Bioscience, Biotechnology and Biochemistry. 66. 2388-2394 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Toyokatzu Yoshida, Yutaka Hayakawa, tsuyoshi Matsui, Toru Nagasawa: "Purification and Characterization of 2,6-Dihydroxybenzoate Decarboxylase Catalyzing Nonoxidative Decarboxylation"Archives of Microbiology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Toyokazu Yoshida, Kohei Fujita, Toru Nagasawa: "Novel reversible indole-3-carboxylate decarboxylase catalyzing nonoxidatide decarboxylation"Bioscience, Biotechnology, and Biochemistry. 66(11). 2388-2394 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Toyokazu Yoshida, Yutaka Hayakawa, Tsuyoshi Matsui, Toru Nagasawa: "Purification and characterization of 2 6-dihydroxybenzoate decarboxylase reversibly catalyzing nonoxidative decarboxylation"Archives of Microbiology. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Toyokzu Yoshida, Kohei Fujita, Tofu Nagasawa: "Novel reversibale indole-3-carboxylate decarboxylase catalyzing nonoxidative decarboxylation"Bioscience, Biotechnology, and Biochemistry. 66(11). 2388-2394 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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