| Project/Area Number |
14560076
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
ARAI Motoo Osaka Prefecture University, Osaka Prefecture University, Agriculture & Biological Sciences, Professor (80081537)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAGUCHI Takashi Osaka Prefecture University, Agriculture & Biological Sciences, Associate Professor (70195056)
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| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | cellulase / glucosidase / gene / yeast / Aspergillus / セルロース / Aspergillus aculeatus / アルコール |
|---|
| Research Abstract |
We tried breeding a yeast which produced alcohol directly from cellulose. Cellulase genes were from Aspergillus aculeatus. Expression vector having different nutrition requirement and different expression modes was constructed. Finally, we succced the double expression of 2 endo cellulases. However, the expression of exo cellulases and beta-glucosidase could not be detected. The expression of beta glucosidase was detected slightly when secretion signal was changed. To know the mechanism of expression control of cellulase genes, the mechanism of CREA was investigated.
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