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Biochemical Elucidation of Function of Citrus Peel Oil

Research Project

Project/Area Number 14560101
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 食品科学・栄養科学
Research InstitutionKOCHI UNIVERSITY

Principal Investigator

UKEDA Hiroyuki  KOCHI UNIVERSITY, Dept.of Bioresources Science, Associate Professor, 農学部, 助教授 (60184991)

Co-Investigator(Kenkyū-buntansha) SAWAMURA Masayoshi  KOCHI UNIVERSITY, Dept.of Bioresources Science, Professor, 農学部, 教授 (20038300)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordscitrus / peel oil / function / tyrosinase / superoxide anion / Maillard reaction
Research Abstract

Citrus peel essential oils are natural flavoring materials of commercial importance. The peel oil from citrus variety not only adds much of the characteristic aroma and flavor to juice products made from that fruit but also has a physiological activity such as antioxidative action and inhibition of carcinogen formation. In order to elucidate the potential of citrus peel essential oils as a functional biomaterial, in the present investigation, I examined several in vitro biochemical activities of essential oils from 13 kinds of citrus fruits. Although yuzu essential oil did not covalently bind to human serum albumin(HAS), the presence clearly promoted the modification of glucose into HSA. The effect of the essential oil on the Maillard reaction partly resulted from the micro-environmental change around Trp-214 of HAS. The enzyme tyrosinase was significantly inhibited by some essential oil such as Eureka lemon, Lisbon and Kiyookadaidai. The comparison of the composition with others suggested the involvement of sabinene, citral, neryl acetate and geranyl acetate. Yuzu essential oil showed a remarkable superoxide anion-scavenging activity. The intensity of the activity showed no significant relationship with DPPH radical-scavenging activity. As the tyrosinase involves in melanin production, the result obtained by the present investigation indicates that some citrus peel oils might be applicable to the field of cosmetology.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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