| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Research Abstract |
Blue-M1 formed from D-xylose-glycine reaction system was isolated and purified by various chromatographies. Blue-M1 was identified as pyrroropyrrole compound having two molecules of pyrrolopyrrole structure, and was a precursor of melanoidins, browned polymer. Antioxidative activities of Blue-M1 were determined by peroxide value using linoleic acid and scavenging activities of hydroxyl and DPPH radicals by ESR. Blue-M1 suppressed the peroxidationof linoleic acid as effectively as melanoidins did. The scavenging activity of Blue-M1 towards hydroxyl and DPPH radicals was also as strong as that of melanoidins. Blue-Mi showed higher activity with increasing concentration. The pyrrlopyrrole ring and a methine bridge between two pyrrolopyrrole rings in Blue-M1 could be related to the activity for radical scavenging activity, but not four carboxyl groups. We investigates the protective effect of Blue-M1 for 2,2'-azobis(2-amidino-propane)dihydrochloride (AAPH)-induced toxicity in COS-1 cells. Blue-M1 decreased the APPH-induced toxicity in COS-1 cells, and the effect was dose-dependent. Furthermore, COS-1 cells were treated with diphenyl-1-pyrenylphosphate (DPPP), as a reagent for the detection of lipid peroxide, and then were cultured in AAPH containing DMEM medium with or without Blue-M1. Blue-M1 prevented the APPH-induced peroxidation of cell membrane on COS-1 cells. These results suggest that Blue-Mi prevents the oxidation cell injury. Therefore, Blue-Mi will be an antioxidant, which protect against the oxidative stress in living systems.
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