| Project/Area Number |
14560156
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|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
|
|---|
| Research Institution | University of Miyazaki |
|---|
Principal Investigator |
SAKAI Masahiro University of Miyazaki, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (20178536)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
|
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| Keywords | DNA / CpG / Carp / immune response / cytokine / CpG / 免疫賦活 / リゾチーム / 白血球 |
|---|
| Research Abstract |
The synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG were evaluated for its ability to enhance the non-specific immune response in carp, Cyprinus carpio. Intraperitoneal injection of CpG-ODNs to fish daily, 3. days, resulted in enhanced responses of phagocytic and nitroblue tetrazolium (NBT) activities in kidney phagocytic cells. This activation of kidney cells was observed for at least 7 days post injection. The serum lysozyme activity also increased in the fish treated with CpG-ODNs. These results indicated that unmethylated CpG DNA enhance the non-specific immune response in carp.
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