• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of simultaneous quantification method for triglyceride molecular species consisting of various kinds of fats and oils

Research Project

Project/Area Number 14560169
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionTokyo University of Marine Science and Technology (2004-2005)
東京水産大学 (2002-2003)

Principal Investigator

WADA Shun  Tokyo University of Marine Science and Technology, Department of Food Science and Technology, Professor, 海洋科学部, 教授 (00089830)

Project Period (FY) 2002 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 2003: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 2002: ¥3,300,000 (Direct Cost: ¥3,300,000)
KeywordsTriglyceride / Molecular Species Analysis / HPLC / Triacontyl Column / Gradient Elution / Calibration Curves / Evaporative Light Scattering Detector / Internal Standard / 高速液体クロマトグラフィー / 油脂 / 定量 / 異性体 / 液体クロマトグラフィー / 脂質栄養 / 構造脂質
Research Abstract

The quantification method for the triglyceride (TG) molecular species in fish oil using tandem jointed high performance liquid chromatography (HPLC)-ultraviolet detector (UV)-evaporative light scattering detector (ELSD) system with gradient elution was developed. The quantification was carried out with internal standard method and the respective calibration curves for TG molecular species were calculated with chromatogram area ratio between internal standard and TG molecular species versus amount ratio between them. The HPLC conditions were shown below.
・UV : 210 nm
・ELSD : Evaporator temperature:110℃, nebrizer temperature:90℃, gas flow rate of ELSD:110℃
・HPLC flow rate : 1.0 mL/min
・Column : Triacontyl (C30) column x 2
・Mobile phase : Acetonitrile (ACN) - 2-propanol (IPA) elution method
・Gradient condition : 0min ACN:IPN=60:40 → 50min ACN:IPN=50:50 → 80min ACN:IPN=60:40 → 100min ACN:IPN=70:30 → 120min finish
・Internal standard : dilinolein
As the results, the second order calibration curves were acquired and all the coefficient of correlations for the calibration curves were higher than 0.99. Several kinds of TG molecular species in bigeye tuna body oil were quantified. The results by UV and ELSD were almost the same for all TG molecular species. In contrast, the results of the chromatogram area percent by UV and ELSD, the conventionally used method for the indication of absolute content of TG molecular species in oil, were completely different. This method would play an important role for the clarification of the nutritional function of fish oil from the TG molecular structure perspectives.

Report

(5 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (7 results)

All 2006 2004 Other

All Journal Article (4 results) Book (2 results) Publications (1 results)

  • [Journal Article] Quantification Method for Triglyceride Molecular Species in Fish Oil with High Performance Liquid Chromatography -Ultraviolet Detector - Evaporative Light Scattering Detector2006

    • Author(s)
      Naohiro Gotoh, Tomoko Aoki, Kyoko Nakayasu, Shigeru Tokairin, Noriko Noguchi, Shun Wada
    • Journal Title

      J.Oleo Sci. 55(in press)

    • NAID

      130000055540

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Quantification Method for Triglyceride Molecular Species in Fish Oil with High Performance Liquid Chromatography - Ultraviolet Detector - Evaporative Light Scattering Detector2006

    • Author(s)
      Naohiro Gotoh, Tomoko Aoki, Kyoko Nakayasu, Shigeru Tokairin, Noriko Noguchi, Shun Wada
    • Journal Title

      J.Oleo Sci. 55 (in press)

    • NAID

      130000055540

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Quantification Method for Triacylglyceride Molecular Species in Fish Oil with High Performance Liquid Chromatography-Ultraviolet Detector2004

    • Author(s)
      Tomoko Aoki, Ikuko Otake, Naohiro Gotoh, Noriko Noguchi, Shun Wada
    • Journal Title

      J.Oleo Sci. 53

      Pages: 285-294

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Quantification Method for Triglyceride Molecular Species in Fish Oil with High Performance Liquid Chromatography - Ultraviolet Detector -Evaporative Light Scattering Detector

    • Author(s)
      Naohiro Gotoh, Tomoko Aoki, Kyoko Nakayasu, Shigeru Tokairin, Noriko Noguchi, Shun Wada
    • Journal Title

      J.Oleo Sci. (submitting)

    • NAID

      130000055540

    • Related Report
      2005 Annual Research Report
  • [Book] II.分析法3.分子種分析(魚油中に含まれるTG分子種を中心に)」水産学シリーズ142 水産機能性脂質-給源・機能・利用-2004

    • Author(s)
      後藤直宏, 和田 俊
    • Publisher
      恒星社厚生閣
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 水産機能性脂質-給源・機能・利用-(日本水産学会監修)2004

    • Author(s)
      後藤直宏, 和田 俊(分担執筆)
    • Total Pages
      179
    • Publisher
      恒星社厚生閣
    • Related Report
      2004 Annual Research Report
  • [Publications] T.Aoki, I.Otake, N Gotoh, N.Noguchi, S.Wada: "Quantification Method for Triglyceride Molecular Species in Fish Oil with High Performance Liquid Chromatography - Ultraviolet Detector"J.Oleo Sci.. (in press). (2004)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi