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Toxification of puffer and tetrodotoxin-associated proteins in liver

Research Project

Project/Area Number 14560170
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionTokyo University of Marine Science and Technology (Tokyo University of Fisheries)

Principal Investigator

NAGASHIMA Yuji  Tokyo University of Marine Science and Technology, Tokyo University of Marine Science and Technology, Associate Professor, 海洋科学部, 助教授 (40180484)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsPuffer / Toxification / Tetrodotoxin / Liver tissue slices / In vitro accumulation / Tetrodotoxin-associated proteins / Transporter / Inhibitors / 麻痺性貝毒
Research Abstract

The liver tissue slices of pufferfish accumulate in vitro tetrodotoxin (TTX), when incubated with minimum essential medium containing TTX. In the case of Takifugu rubripes liver slices incubated at a concentration of 25μg TTT/ml, TTX of 3.9μg/g was first detected at 2h and increased to 15.0μg/g at 48h. The TTX content accumulated was first detected at 2h and increased to 15.0μg/g at 48h. The TTX content accumulated was not decreased, even when the slices were further incubated without TTX for additional 48h. On the contrary, in the cases of the liver slices from parrot-bass Oplegnathus fasciatus, green ling Hexagrammos otakii and filefish Thamnaconus modestus incubated at a concentration of 25μg TTX/ml, TTX of 3-4μg/g was detected even at 0.5h. However, no significant change in TTX contents was recognized during the incubation for 48h. Further incubation of the filefish liver slices without TTX for additional 48h did not decrease the TTX content. It is unlikely that the liver slices of slices of filefish as well as pufferfish rapidly excrete TTX.
Effect of inhibitors of transport system on in vitro accumulation of TTX in T.rubripes liver tissue slices was examined. Addition of p-aminohippuric acid and carnitine to the culture media significantly decreased in TTX content accumulated in the puffer liver tissue slices while that of tetraethylammonium did not, suggesting the involvement of an organic ion transporter in TTX accumulation in puffer liver.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Yuji Nagashima: "In vitro accumulation of tetrodotoxin in puffer fish liver tissue slices"Toxicon. 41. 569-574 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yuji NAGASHIMA: "In vitro accumulation of tetrodotoxin in pufferfish liver tissue slices."Toxicon. 41. 569-574 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yuji Ngashima: "In vitro accumulation of tetrodotoxin in pufferfish liver tissue slices"Toxicon. 41・5. 569-574 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yuji Nagashima: "In vitro accumulation of tetradotoxin in pufferdish liver tissue slices"Toxicon. 41・5. 569-574 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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