| Project/Area Number |
14560221
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Zootechnical science/Grassland science
|
|---|
| Research Institution | Hirosaki University |
|---|
Principal Investigator |
TOBA Takahiro Hirosaki University, Faculty of Agriculture and Life Science, Professor, 農学生命科学部, 教授 (10108483)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
|---|
| Keywords | Lactobacillus crispatus / hydrogen peroxide / bifidobacteria / PCR / Lactobacillus gasseri / S-layer protein |
|---|
| Research Abstract |
Adhesion assay was performed using Lactobacillus and Bifidobacterium strains isolated from chicken and human faeces to select the strains which strongly adhered to chicken crop epithelium, human intestinal Caco-2 cells and HT-29 cells, and immobilized extracellular matrix proteins. For rapid identification of Lactobacillus crispatus we newly developed PCR primers targeting S-layer protein gene(Horie et al.2002). Adhesive lactobaciller and bifidobacterial strains inhibited the adhesion of pathogens such as Salmonella to Caco-2 cells Adhesion of L.crispatus and L.gasseri strains tested were induced on plate cultures under anaerobic conditions. A 46.5kDa S-layer protein(named as LbsA) was identified as an adhesin of highly adhesive L.crispatus MH 3 1 5 strain. The DNA region coding for LbsA was cloned and sequenced. Alignment comparisons of the predicted amino acid sequence with S-layer proteins of other Lactobacillus strains suggested the presence of laminin-binding site in LbsA (Mukai et al.2003). An 58.7kDa surface protein was identified an putative adhesin of L.gasseri MH 411 by Western bolting assay.
We also selected hydrogen peroxide(H_2O_2)-producing Lactococcus lactis subsp.lactis and L.plantarum strains from foods. They produced >300ppm H_2O_2 under the condition tested. One of these strains, L.lactis subsp.lactis AI62 inhibited pathogens such as Listeria ivanovii, Yersinia enterocolitica and Aeromonas hydrophila efficiently(Ito et al.2003).
|
