| Project/Area Number |
14560223
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
TANIGUCHI Kohzo Hiroshima University, Graduate School of Biosphere Science, Professor, 大学院・生物圏科学研究科, 教授 (30093777)
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| Co-Investigator(Kenkyū-buntansha) |
OBITSU Taketo Hiroshima University, Graduate School of Biosphere Science, Associate Professor, 大学院・生物圏科学研究科, 助教授 (30194632)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Splanchnic tissues / Grazing sheep / Oxygen consumption / Energy intake / Nitrogen metabolism |
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| Research Abstract |
The objectives were to investigate energy intake and nitrogen (N) metabolism of grazing sheep with net flux of N and oxygen consumption by the portal drained viscera (PDV) and liver through three experiments over two years. Wethers with chronic indwelling catheter in the portal, hepatic and mesenteric veins and the abdominal aorta were used under housing with restricted feeding (Exp. 1 and 2) and grazing with adlibitum intake (Exp. 3) conditions
Exp. 1 : The sheep were fed a diet consisting of forage (70) and concentrate (30) at four levels, 0.5 to 1.76 times maintenance energy (M). Digestible energy and N intakes and N retention of sheep linearly increased with the feeding level. Ammonia N absorption by PDV also increased linearly, while α-amino N absorption, starting from null at 0.5M, increased quadratically
Exp. 2 : The sheep were fed forage (1.0M) plus four amounts of barley supplement (0 to 1.0M). Digestible energy and N intakes increased linearly, but N retention quadratically responded to the supplement level. The PDV release of α-amino N increased linearly with the supplement level, although the a -amino N release by the total splanchnic tissues (TSP) was almost constant due to the increase in the liver uptake. The increased supplement linearly increased the remove of urea N by PDV, but did not affect urea N synthesis by the liver and ammonia N absorption by PDV
Exp. 3 : The sheep were grazed on a pasture during May and June. The α-amino N release by TSP increased with barley supplement, and was less in June compared with May. Digestible energy intake, estimated with a linear regression between the PDV oxygen consumption and energy intake in Exp. 1 and 2, was greater in June, and not affected by barley supplement
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