| Project/Area Number |
14560228
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Nihon University |
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Principal Investigator |
ITOH Takatoshi Nihon University, College of Bioresource Sciences, Department of Animal Science and Resources, Professor, 生物資源科学部, 教授 (80005610)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Lactic acid bacteria / Lactobacillus acidophilus / Lactobacillus gasseri / Probiotics / Fermented milk / Streptococcus thermophilus / プロバイオティク / Lactobacillus accidophilus / プロテアーゼ / カゼイン |
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| Research Abstract |
Lactobacillus acidophilus group lactic acid bacteria is a dominant lactobacilli in the human intestinal tract, and expected as useful probiotic strains. However, they usually grow poorly in milk than other yogurt starter lactic cultures. Therefore, the factors relating with the growth rate of L.acidophilus in milk were investigated. The amount of the remaining non-protein nitrogen components in fermented reconstituted milk were parallel to the growth rate of the strains. The relationship between the protease activity and growth rate of L.acidophilus in milk were investigated. The strains in L.acidophilus group which can grow well in milk were known to secrete the exocellular proteases at early stage of the cell growth, and the activity was kept constant while further incubation period. The results indicated that the high exocellular protease producing strains can preferentially grow in milk, because the low molecular nitrogen components should be supplied through hydrolysis of milk proteins by bacterial own proteases.
Addition of casein hydrolyzed by 3 proteases showed all positive promoting effects on the acid production. In particular, casein treated with Protease p (Amano enzyme, Japan) showed the highest effect. The results indicated that the addition of the enzymatically digested casein to milk is a very useful way to accelerate the growth of L.acidophilus.
The co-culture of the S.thermophilus with some strain of L.acidophilus was known to promote the growth of the L.acidophilus in milk. It is suggested the presence of some symbiotic relationship between two strains and the co-culture is a possible method for the production of probiotic fermented milk using L.acidophilus.
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