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Studies on novel growth hormone (GH) secretory mechanisms by ghrelin in ruminants

Research Project

Project/Area Number 14560230
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionIwate University

Principal Investigator

HASHIZUME Tsutomu  Iwate University, Agriculture, Professor, 農学部, 教授 (60124533)

Co-Investigator(Kenkyū-buntansha) KASUYA Etsuko  National Institute of Agrobiological Science, Researcher, 主任研究員
SANO Hiroaki  Iwate University, Agriculture, Professor, 農学部, 教授 (20196306)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsGhrelin / GH / Growth Hormone / Cattle / Pig / Goat
Research Abstract

Ghrelin, a novel endogenous growth hormone (GH) secretagogue, has been shown to exert very potent and specific GH-releasing activity in rats and humans. However, little is known about its GH-releasing activity and endocrine effects in domestic animals. To clarify the effects of ghrelin on GH secretion in ruminants, GH-releasing effects of ghrelin were examined in vitro and in vivo in goats and cattle. GH-releasing effects of human ghrelin (hGhrelin) and rat ghrelin (rGhrelin) on bovine anterior pituitary (AP) cells were compared with those of GH-releasing hormone (GHRH) in vitro. hGhrelin and rGhrelin significantly stimulated GH release from the cultured cells at doses from 10^<-10> to 10^<-7> M and from 10^<-9> to 10^<-7> M, respectively (P<0.05). However, GH-releasing effects of ghrelin were weaker than that of GHRH. When hGhrelin (10^<-8>M) and GHRH (10^<-8>M) were added together, the release of GH induced by both peptides was significantly greater than that by hGhrelin alone (P<0.0 … More 5), and tended to be greater than that by GHRH alone. Somatostatin (SS, 10^<-7> M) significantly blunted GH release induced by hGhrelin (10^<-8>M) and GHRH (10^<-8>M) (P<0.05). In the presence of SS, additive effects of hGhrelin and GHRH on the release of GH were observed (P<0.05). These in vitro results in cattle were similar to those in pigs. To clarify the effect of ghrelin on GH secretion in vivo in ruminants, plasma GH responses to intra-arterial and intra-hypothalamic injections of rGhrelin were examined in goats and cattle. The infra-arterial injection of 3 μg/kg BW of rGhrelin in ovariectomized goats significantly increased plasma GH concentrations (P<0.05). However, the secretory response to 3 μg/kg BW of rGhrelin was weaker than that of GHRH (0.25μg/kg BW). An infusion of 10 nmol of ghrelin into the medial basal hypothalamus (arcuate nucleus) significantly stimulated the release of GH in male calves (P<0.05). These results show that ghrelin is one of potent physiological GH secretagogues in ruminants. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (9 results)

All 2005 2003 Other

All Journal Article (7 results) Publications (2 results)

  • [Journal Article] Effects of ghrelin on growth hormone secretion in vivo in ruminants.2005

    • Author(s)
      T.Hashizume et al.
    • Journal Title

      Regulatory Peptides 126・1-2

      Pages: 61-65

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of ghrelin on growth hormone secretion in vivo in ruminants.2005

    • Author(s)
      T.Hashizume et al.
    • Journal Title

      Regulatory Peptides 126

      Pages: 61-65

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of ghrelin on growth hormone secretion in vivo in Ruminants.2005

    • Author(s)
      T.Hashizume et al.
    • Journal Title

      Regulatory Peptides 126・1-2

      Pages: 61-65

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of ghrelin on growth hormone secretion from cultured adenohypophysial cells in cattle.2003

    • Author(s)
      T.Hashizume et al.
    • Journal Title

      Endocrine Journal 50・3

      Pages: 289-295

    • NAID

      10011210938

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of Ghrelin on growth hormone secretion from cultured adenohypophysial cells in pigs.2003

    • Author(s)
      T.Hashizume et al.
    • Journal Title

      Domestic Animal Endocrinology 24・3

      Pages: 209-218

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of ghrelin on growth hormone secretion from cultured adenohypophysial cells in cattle.2003

    • Author(s)
      T.Hashizume et al.
    • Journal Title

      Endocrine Journal 50

      Pages: 289-295

    • NAID

      10011210938

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of Ghrelin on growth hormone secretion from cultured adenohypophysial cells in pigs.2003

    • Author(s)
      T.Hashizume et al.
    • Journal Title

      Domestic Animal Endocrinology 24

      Pages: 209-218

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] T.Hashizume et al.: "Effects of Ghrelin on growth hormone secretion from cultured adenohypophysial cells in cattle"Endocrine. Journal. 50・3. 289-295 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Hashizume: "Effects of ghrelin on growth hormone secretion from cultured adenohypophysial cells in pigs"Domestic Animal Endocrinology. 24巻3号(印刷中). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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