Signal transduction mechanisms on the regulation of the acrosome reaction and the motility of fowl spermatozoa.

• Project/Area Number: 14560236
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: University of Miyazaki
• Principal Investigator: ASHIZAWA Koji University of Miyazaki, Fac.Agric., Professor, 農学部, 教授 (60128353)
• Project Period (FY): 2002 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥4,000,000 (Direct Cost: ¥4,000,000); Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000); Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: spermatozoa / protein phosphatase / protein kinase / regulation of motility / phosphorylation / dephosphorylation / signal transduction / acrosome reaction / 鞭毛運動 / PP1 / カルパイン

Research Abstract

In order to investigate what sort of protein phosphatases are involved in the regulation of fowl sperm motility and acrosome reaction(AR), the influence of certain protein phosphatase inhibitors on the motility and AR of spermatozoa at 40℃ was examined. Regardless of whether the inner perivitelline layers(IPVL) present or not, the motility of spermatozoa at 40℃ was restored by the addition of 10-100 nM tautomycin, but did not almost restored by the addition of 1000 nM tautomtcin. When IPVL was present, AR was stimulated by the addition of tautomycin. Furthermore, regardless of whether IPVL present or not, an addition of 2 mM Ca^<2+> after the addition of tautomycin (100 nM) was effective for the recover of motility at 40℃, and the AR was also stimulated. On the other hand, an addition of calyculin A recovered the motility of spermatozoa, and stimulated the AR. An addition of 2 mM Ca^<2+> after the addition of calyculin A (100 nM) was effective for the recover of motility at 40℃, and th e AR was also stimulated. In addition, regardless of whether IPVL present or not, the motility of spermatozoa at 40℃ was not almost restored by the addition of okadaic acid, compared with those of the addition of calyculin A. However, when IPVL was present, AR was stimulated by the addition of okadaic acid. Regardless of whether IPVL present or not, the motility of spermatozoa at 40℃ was not restored by the addition of genisteine. The AR was stimulated by the addition of genisteine within the range of 0-10 μM, but did not almost stimulated by the addition of 100 μM genisteine. Furthermore, regardless of whether IPVL present or not, an addition of 2mM Ca^<2+> after the addition of genisteine within the range of 0-10 μM was effective for the recover of motility at 40℃.
From the results described above, it is suggested that the motility of fowl spermatozoa may be regulated by PP1, but the AR of spermatozoa may be regulated by PP1 and PP2A, In addition, the motility and AR of spermatozoa may be regulated by PTK or PKC. But, it appears that PKA is not involved with the motility and AR of spermatozoa.

Research Products

• [Journal Article] Protein phosphatase-type 2B is involved in the regulation of the acrosome reaction but not in the temperature-dependent flagellar movement of fowl spermatozoa. (2004)
  • Author(s): Ashizawa et al.
  • Journal Title: Reproduction 128・2 Pages: 783-787
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Protein phosphatase-type 2B is involved in the regulation of the acrosome reactionbut not in the temperature-dependent flagellar movement of fowl spermatozoa. (2004)
  • Author(s): Ashizawa et al.
  • Journal Title: Reproduction 128 Pages: 783-787
  • Description: 「研究成果報告書概要(欧文)」より