| Project/Area Number |
14560249
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Yamaguchi University |
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Principal Investigator |
IWATA Hiroyuki Yamaguchi Univ., Fac.Agr., Professor, 農学部, 教授 (40193750)
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| Co-Investigator(Kenkyū-buntansha) |
OKUDA Masaru Yamaguchi Univ., Fac.Agr., Associate Professor, 農学部, 助教授 (10325243)
KATO Hirotomo Yamaguchi Univ., Fac.Agr., Associate Professor, 農学部, 助教授 (00346579)
KISO Yasuo Yamaguchi Univ., Fac.Agr., Professor, 農学部, 教授 (10142374)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | induction of placentation / microarray / food animal production / gene therapy / Lentivirus vector / siRNA methods / tissue specifc knockout |
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| Research Abstract |
The molecule, which is available for embryonic implantation in mice, has not been discovered without the leukemia inhibitory factor (LIF). So, the molecular dynamics in the implantation was analysed by microarray technology using the LIF KO and the delayed implantation mice. The microarray analysis in this study used MG_U74Av2 array (Affymetrix Co.), which detected the 12,489 genes. We detected the repression of gene expression in 50 genes 4 days after pregnancy in the LIF KO and delayed implantation mice. Next, mice with delayed implantation were produced, and a role of LIF at the implantation period was clarified to be essential for the event. About 80% of the selected 50 genes could be controlled by only LIF. As for LIF treatment, the effects of LIF were arisen in various times from several minutes to 6 hours after increase of LIF in the blood. Furthermore, expression and functional analyses of CD9 revealed that CD9 was increased in gene and protein levels at the implantation period and the inhibition of implantation was observe in CD9 KO mice transplanted with fertilized eggs. As fox gene transfer technology using Lentivirus vector, AP and ZAP genes were introduced in mice and the expressions of these genes were confirmed
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