Molecular pathobiological study on neuropathogenicity of a new lethal neurotropic herpesvirus
| Project/Area Number |
14560264
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | GIFU UNIVERSITY |
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Principal Investigator |
FUKUSHI Hideto Gifu University, Faculty of Agriculture, Professor, 農学部, 教授 (10156763)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Tsuyoshi Gifu University, Faculty of Agriculture, Assistant Professor, 農学部, 助教授 (70210367)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | herpesvirus / horse / neuropathogenicity / bacterial artificial chromosome |
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| Research Abstract |
We investigated genes involved in the control of virus growth and pathogenesis of equine herpesvirus 9 (EHV-9) which shows specific neuropathogenicity.
(1) We constructed BAC by introducing the replication origin into EHV-9 genome by transposon (Tn) technique. We constructed Tn-OriR which is a Tn contaiting a replication origin in Escherichi coli. The relication origin is derived from R6K plasmid. Introducing Tn-OriR into EHV-9 genome DNA in vitro, E..coli was transformed. We found that EHV-9 genome could be cloned as BASC by this method. It was impossible to introduce BAC into a specific site because of the Tn. Therefore we obtained BAC clones as a library The BAC library should be usefule to analyze gene functions of EHV-9.
(2) We confirmed that the glycoprotein E (gE) mutant lost the pathogenicity completely and the revretant showed the frill pathogenicity. A product of gE gene should have the determinated role in the pathogenicity of EHV-9.
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Report
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Research Products
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