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Studies on bioflim formation and survival mechanisms of Campylobacter species in foods

Research Project

Project/Area Number 14560268
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionUniversity of Miyazaki

Principal Investigator

MISAWA Naoaki  University of Miyazaki, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (20229678)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2002: ¥300,000 (Direct Cost: ¥300,000)
KeywordsCampylobacter / Food / Biofilm / Survival / Gene / バイオフイルム
Research Abstract

We observed that Campylobacter jejuni strain 81-176, a strong slime producer, showed spreading and highly mucoid colonies on blood agar plates, and formed biofilm on the bottom of a glass flask after stationary cultivation in Brucella broth. The present study was performed to examine the mode of C. jejuni adherence to a smooth surface. When bacterial suspensions in Brucella broth were incubated in a microplate well, microcolonies were formed on the coverslip by 2 h after incubation. The microcolonies gradually grew and formed a biofilm of net-like connections by 6 h. Transmission electron microscopy indicated that massive amounts of extracellular material were masking the cell surface and bound ruthenium red, suggesting the presence of a poiysaccharide moiety. Scanning electron microscopy indicated that the flagella acted as bridges, forming a net-like connection between the organisms. To determine the genes associated with biofilm formation, several mutants were constructed from strain 81-176 by natural transformation-madiated allelic exchange. As aflagellate (flaAB and flbA) and flagellate but nonmotile (pflA and motA) mutants did not form a biofilm exhibited by the wild type strain even though capsular-like polysaccharide was expressed on the cell surface. In contrast, kpsM and peb1 mutants which lack capsular-like polysaccharide and a outer membrane protein, respectively, retained the ability of biofilm formation. These findings suggest that flagella-mediated motility but not capsular-like polysaccharide and PEB 1 protein is needed for biofilm formation in vitro.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 三澤尚明: "カンピロバクター感染症の新展開"日本食品微生物学会雑誌. 20. 91-97 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jeon, B. et al.: "Effects of quorum sensing on flaA transcription and autoagglutination in Campylobacter jejuni"Microbiology and Immunology. 47. 833-839 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 三澤尚明 他: "カンピロバクター属(ジェジュニ/コリ)(総説)"日本臨床. 61(増刊号3). 727-731 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 三澤尚明 他: "人および鶏肉由来Campylobacter jejuni HS:2およびHS:19血清型株のPCR-RFLP法による遺伝子解析"日本獣医師会雑誌. 56. 471-475 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Misawa, N. et al.: "Isolation and characterization of Campylobacter, Helicobacter, and Anaerobiospirillum strains from a puppy with bloody diarrhea"Veterinary Microbiology. 87. 353-364 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Misawa, N.: "New aspects of human campylobacteriosis"Jpn. J. Food Microbiol.. 20. 91-97 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jeon, B.: "Effects of quorum sensing on flaA transcription and autoagglutination in Campylobacter jejuni"47. 833-839 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Misawa, N.: "Campylobacter jejuni/coli"61. 727-731 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Misawa, N.: "Molecular analysis of Campylobacter jejuni HS : 2 and HS : 19 strains isolated from human and flesh chicken meat by PCR-RFLP"56. 471-475 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Misawa, N.: "Isolation and characterization of Campylobacter Helicobacter, and Anaerobiospirillum strains from a puppy with bloody diarrhea"87. 353-364 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 三澤尚明: "カンピロバクター感染症の新展開"日本食品微生物学会雑誌. 20・3. 91-97 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Jeon, B., Itoh, K., Misawa, N., Ryu, S.: "Effects of quorum sensing on flaA transcription and autoagglutination in Campylobacter jejuni"Microbiology and Immunology. 47・11. 833-839 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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