| Project/Area Number |
14560270
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Rakuno Gakuen University |
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Principal Investigator |
IZUMISAWA Yasuharu Rakuno Gakuen University, School of Veterinary Medicine, Department of small animal clinics, Professor, 獣医学部, 教授 (60193380)
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| Co-Investigator(Kenkyū-buntansha) |
KIRISAWA Rikio Rakuno Gakuen University, School of Veterinary Medicine, Department of Microbiology, Associate Professor, 獣医学部, 助教授 (70153252)
HAYASHI Masanobu Rakuno Gakuen University, School of Veterinary Medicine, Department of Veterinary Radiology, Professor, 獣医学部, 教授 (10130337)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Tumors / Radiation therapy / Apoptosis / Cytokine / Radiation sensitivity / Gene expression |
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| Research Abstract |
We investigated the induction of apoptosis in tumor cells by radiation therapy of small animals, and relationship between induction of apoptosis and side effects during cancer treatment. We also investigated a role of cytoldnes in induction of apoptosis and normal tissue injury.
1.Complete reaction or partial reaction were observed in fifty percentage of clinical cases of cancers of small animals by radiation therapy alone or by combination of radiation therapy and chemotherapy. Acute skin problems such as hair loss and skin changes were observed in 30 to 40% of treated animals during cancer treatiment. Late side effects such as skin ulcer were also observed after irradiation with high doses of X-rays.
2.The side effects of radiation treatment vary from patient to patient. We developed a modified comet assay for estimation of radiosensitivity of individual dogs using peripheral blood cells.
3.Copper plays an important role in angiogensis. In the murine fibrosarcoma xenograft models, the copper chelating agents induced apoptosis in tumor cells in vivo and in vitro.
4.For assessment of contribution of cytokines to side effects, especially skin problems, by radiation treatment, we development a sandwich ELISA method for measurements of animal cytokines using recombinant cytokines.
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