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Epidemiological significance of Erysipelothrixisolates from the tonsils of healthy cattle

Research Project

Project/Area Number 14560274
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionNippon Veterinary and Animal Science University

Principal Investigator

SAWADA Takuo  Nippon Veterinary and Animal Science University, Faculty of Veterinary Medicine, Professor, 獣医学部, 教授 (30226071)

Co-Investigator(Kenkyū-buntansha) KATAOKA Yasushi  Nippon Veterinary and Animal Science University, Faculty of Veterinary Medicine, Associate Professor, 獣医学部, 助教授 (60277663)
YOSHIDA Takaharu  Nippon Veterinary and Animal Science University, Faculty of Veterinary Medicine, Lecturer, 獣医学部, 講師 (20060527)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsErysipelothrix / healthy cattle / tonsils / serovars / antibody / PCR / pathogenicity / molecular identification / 菌種 / 牛特異的菌種 / マウス / 豚 / 選択培地 / 保菌状況
Research Abstract

To clarify the epidemiological significance of Erysipelothrixiofection in cattle, antibody distribution in cattle and prevalence, serotyping, pathogenicity and molecular identification of the isolates from the tonsils of healthy cattle were studied. The results obtained are as follows.
1.Growth agglutination test indicated that most of the sera collected from 854 cattle in 9 prefectures of Japan, from Hokkaido to Okinawa, between 1988 and 1992 showed positive reactions, and the antibody titers ranged from below 4 to above 128. Seventy-seven percent of the sera showed titers of above 32. The antibody detection indicated that Japanese cattle had been infected with Erysipelothrix.
2.Seventy-nine isolates of Erysipelothrix species were obtained from 1,236 healthy cattle at slaughter houses in Yamagata, Miyagi, Tokyo and Nagasaki prefecture of Japan.
3.Serotyping results of the 79 Erysipelothrixisolates demonstrated that 43 isolates could be classified into 9 serovars, 1b,2,3,5,9,12,13,19 and … More 21 but the remaining 36 isolates were untypable.
4.The pathogenicity tests demonstrated that 5(6.3%) and 21(25.3%) isolates of Erysipelothzix from cattle were lethal and induced clinical signs for mice, respectively. Four of the former 5 isolates induced localized or generalized urticarial lesions in swine. All the swine inoculated with 8 isolates virulent for mice developed fever and antibody titers. The results indicates that the bovine isolates are pathogenic.
5.PCR performed with the DNAs from the 79 bovine isolates demonstrated that these isolates were identified as 3 Erysipelothrix species, E.rhusiopathiae, E.tonsillarum and Erysipelothrix sp.strain 1. All the 36 isolates serologically untypable were identified as Erysipelothrix sp.strain 1. Genetic variations were recognized in the species of the same serovars. Erysipelotrhix sp.strain 1 is likely bovine specific. A correlation between pathogenicity and E.rhusiopathiae was observed. Identification of the species based on serovars and DNA homology among reference strains for serotyping was not always correlated with the results by the PCR. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (14 results)

All 2004 2003 2001 2000 Other

All Journal Article (10 results) Book (1 results) Publications (3 results)

  • [Journal Article] Pathogenicity for mice and swine of Erysipelothrix isolates from the tonsils of healthy cattle2003

    • Author(s)
      Hassanein, R., Sawada, T., Kataoka, Y., Gadallah, A., Suzuki, Y., Takagi, M., Yamamoto, K.
    • Journal Title

      Veterinary Microbiology 91

      Pages: 231-238

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular identification of Erysipelothrix isolates from the tonsils of healthy cattle by PCR2003

    • Author(s)
      Hassanein, R., Sawada, T., Kataoka, Y., Gadallah, A., Suzuki, Y.
    • Journal Title

      Veterinary Microbiology 95

      Pages: 239-245

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Pathogenicity for mice and swine of Erysipelotrhix isolates from the tonsils of healthy cattle2003

    • Author(s)
      Hassanein, R., Sawada, T., Kataoka, Y., Gadallah, A., Suzuki, Y., Takagi, M., Yamamoto, K.
    • Journal Title

      Vet.Microbiol. 91

      Pages: 231-238

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular identification of Erysipelothrix isolates from the tonsils of healthy cattle by PCR2003

    • Author(s)
      Hassanein, R., Sawada, T., Kataoka, Y., Gadallah, A., Suzuki, Y.
    • Journal Title

      Vet.Microbiol. 95

      Pages: 239-245

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distribution of antibody against Erysipelothrix rhusiopathiae in cattle2001

    • Author(s)
      Sawada, T., Hassanein, R., Yamamoto, T., Yashida, T.
    • Journal Title

      Clinical and Diagnostic Laboratory Immunology 8

      Pages: 624-627

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Serovars of Erysipelothrix species isolated from the tonsils of healthy cattle in Japan2001

    • Author(s)
      Hassanein, R., Sawada, T., Kataoka, Y., Itoh, K., Suzuki, Y.
    • Journal Title

      Veterinary Microbiology 82

      Pages: 97-100

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distribution of antibody against Erysipelothrix rhusipathiae in cattle2001

    • Author(s)
      Sawada, T., Hassanein, R., Yamamoto, T., Yoshida, T.
    • Journal Title

      Clin.Diagn.Lab.Immunol. 8

      Pages: 624-627

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Serovars of Erysipelothrix species isolated from the tonsils of healthy cattle in Japan2001

    • Author(s)
      Hassanein, R., Sawada, T., Kataoka, Y., Itoh, K., Suzuki, Y.
    • Journal Title

      Vet.Microbiol. 82

      Pages: 97-100

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prevalence of Erysipelothrix species in healthy, slaughtered cattle2000

    • Author(s)
      Hassanein, R., Sawada, T., Kataoka, Y., Ali, M., Itoh, K., Suzuki, Y.
    • Journal Title

      Journal of Veterinary Epidemiology 4

      Pages: 71-79

    • NAID

      10022000686

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prevalence of Erysipelothrix species in healthy, slaughtered cattle2000

    • Author(s)
      Hassanein, R., Sawada, T., Kataoka, Y., Ali, M., Itoh, K., Suzuki, Y.
    • Journal Title

      J.Vet.Epidemiol. 4

      Pages: 71-79

    • NAID

      10022000686

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 人獣共通感染症2004

    • Author(s)
      澤田拓士(共著)
    • Total Pages
      447
    • Publisher
      医薬ジャーナル
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Hassanein, R., Sawada, T., Kataoka, Y., Gadallah, A., Suzuki, Y., Takagi, M., Yamamoto, K.: "Pathogenicity for mice and swine of Erysipelothrix isolates from the tonsils of healthy cattle"Veterinary Microbiology. 91. 231-238 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sawada, T., Hassanein, R., Yamamoto, T., Yoshida, T.: "Distirbution of antibody against Erysipelothrix rhusiopathiae in cattle"Clinical and Diagnostic Laboratory Immunology. 8(3). 624-627 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hassanein, R., Sawada, T., Kataoka, Y., Ali, M., Itoh, K., Suzuki, Y.: "Prevalence of Erysipelothrix species in Healthy, slaughtered cattle"Journal of Veterinary Epidemiology. 4(2). 71-79 (2000)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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