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Induction of DNA damage and its fixation as chromosome aberrations by azo dyes in mouse multiple organs

Research Project

Project/Area Number 14560277
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionHachinohe National College of Technology

Principal Investigator

SASAKI Yu  Hachinohe National College of Technology, Department of Chemical and Biological Engineering, Professor, 物質工学科, 教授 (20259790)

Co-Investigator(Kenkyū-buntansha) TSUDA Shuji  Iwate University, Faculty of Agriculture, Laboratory of Veterinary Public Health, Professor, 農学部・獣医学科, 教授 (60281953)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsazo dyes / food dyes / comet assay / genotoxicity / Miconucleus
Research Abstract

We have shown that some azo food dyes, such as Amaranth, show genotoxicity in mouse gastrointestinal tract (GI-tract) using the comet assay, in spite of lack of their rodent carcinogenicity. The purpose of this study is to query whether this discrepancy is due to the difference of treatment schedule between genotoxicity and carcinogenicity studies. Six kind of food dyes that were positive in the comet assay were given to groups of four male ddY by single and triple gavage at 100-2000 mg/kg/day and in the drinking wate at 0.5-2.5% for 6 days. Although azo food dyes were positive in the stomach and colon after single gavage, they were negative after triple dosing. When azo dyes in the drinking water were continuously dosed, they induced DNA damage in the GI-tract after 1-2 day drinking and their induced DNA damage decreased with drinking period and returned to control level after 6 day drinking. On the other hand, 1,2-Dimethylhydrazine (DMH), which is carcinogenic to the colon and liver following to their gavage dosing, yielded DNA damage at these sites after triple dosing. p-dimethylaminoazobenzene (DAB) and DMH, both are carcinogenic to the liver following to feeding and drinking, were still genotoxic in the liver following to 6-day feeding or drinking. Therefore, our present result suggested good correlation between carcinogenicity and increasing tendency of genotoxicity with dosing period in the GI-tract and liver when chemicals are given to mice continuously in the drinking-water or diet.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (12 results)

All 2003 Other

All Journal Article (9 results) Publications (3 results)

  • [Journal Article] Effect of in vitro exposure time on comet assay results2003

    • Author(s)
      Y.F Sasaki, K.Sekihashi, H.Saitoh, S.Hori, M.Nakagawa, M.Miyagawa
    • Journal Title

      Environmental.Mutagen Research 25

      Pages: 83-86

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Detection of in vivo genotoxicity of endogenously formed N-nitrosocompounds and its suppression by ascorbic acid, teas and fruit juices.2003

    • Author(s)
      K.Ohsawa, S.Nakagawa, M.Kimura, C.Shimada, S.Tsuda, K.Kabasawa, S.Kawaguchi, Y.F.Sasaki
    • Journal Title

      Mutation Research 539

      Pages: 65-76

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of in vitro exposure time on comet assay results2003

    • Author(s)
      Y.F.Sasaki, K.Sekihashi, H.Saitoh, S.Hori, M.Nakagawa, M.Miyagawa
    • Journal Title

      Environmental. Mutagen Research 25

      Pages: 83-86

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Detection of in vivo genotoxicity of endogenously formed N-nitrosocompounds and its suppression by ascorbic acid, teas and fruitjuices2003

    • Author(s)
      K.Ohsawa, S.Nakagawa, M.Kimura, C.Shimada, S.Tsuda, K.Kabasawa, S.Kawaguchi, Y.F.Sasaki
    • Journal Title

      Mutation Research 539

      Pages: 65-76

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vive and in vitro re-evaluation of genotoxic potential of Kojic acid

    • Author(s)
      S.Okutani, N.Yokohama, K.Mitsumori, Y.F.Sasaki
    • Journal Title

      Environmental Molecular Mutagenesis (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Schedule of administration affect comet assay-detected genotoxicity of food dyes in mouse gastrointestinal organs

    • Author(s)
      N.Yokohama, A.Zenke, S.Tsuda, Y.F.Sasaki
    • Journal Title

      Mutation Research (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Schedule of administration affects comet assay-detected genotoxicity of food dyes in mouse gastrointestinal organs

    • Author(s)
      N.Yokohama, A.Zenke, S.Tsuda, Y.Sasaki
    • Journal Title

      Mutation Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vivo and in vitro re-evaluation of genotoxic potential of Kojic acid

    • Author(s)
      S.Okutani, N.Yokohama, K.Mitsumori, Y.F.Sasaki
    • Journal Title

      Environ. Mo.l. Mutagen. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Schedule of administration affect comet assay-detected genotoxicity of food dyes in mouse gastrointestinal organs

    • Author(s)
      N.Yokohama, A.Zenke, S.Tsuda, Y.F.Sasaki
    • Journal Title

      Mutation Research (in press)

    • Related Report
      2004 Annual Research Report
  • [Publications] Y.F Sasaki, K.Sekihashi, H.Saitoh, S.Hori, M.Nakagawa, M.Miyagawa: "Effect of in vitro exposure time on comet assay results"Environmental.Mutagen Research. 25. 83-86 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Ohsawa, S.Nakagawa, M.Kimura, C.Shimada, S.Tsuda, K.Kabasawa, S.Kawaguchi, Y.F.Sasaki: "Detection of in vivo genotoxicity of endogenously formed N-nitrosocompounds and its suppression by ascorbic acid, teas and fruit juices."Mutation Research. 539. 65-76 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Saga, K.Sekihashi, S.Okutani, N.Yokohama, K.Mitsumori, Y.F.Sasaki: "In vivo and in vitro re-evaluation of genotoxic potential of Kojic acid"Environmental Molecular Mutagenesis. (in press). (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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