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A STUDY OF CELLULAR NETWORKS WITHIN THE CENTRAL NERVOUS SYSTEM BY USING CONNEXIN45 GENE KNOCKOUT-LACZ GENE KNOCKIN MICE.

Research Project

Project/Area Number 14570016
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKURUME UNIVERSITY (2003)
Kyushu University (2002)

Principal Investigator

NAKAMURA Kei-ichiro  KURUME UNIVERSITY, DEPARTMENT OF ANATOMY & HISTOLOGY, ASSOCIATE PROFESSOR, 医学部, 助教授 (20172398)

Co-Investigator(Kenkyū-buntansha) NISHII Kiyomasa  KYUSHU UNIVERSITY GRADUATED SCHOOL OF MEDICAL SCIENCES, DEPARTMENT OF DEVELOMENTAL MOLECULAR ANATOMY, RESEARCH ASSOCIATE, 大学院・医学研究院, 助手 (20264020)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsCONNEXIN45 / GAP JUNCTION / KNOCKOUT MOUSE / LACZ / GFP / STEM CELL / IMMUNOCYTOCHEMISTRY / ELECTRON MICROSCOPY / knockout mice / central nervous system / lac Z / confocal laser scanning microscopy
Research Abstract

We have investigated connexin45 (Cx45) gene knockout-lacZ gene knockin mice in order, to clarify Cx45 expressing cells in the central nervous system. 1. X-gal staining of those animal tissues enable us to visualize Cx45 expressing cells by the blue coloring. We performed X-gal staining on 1mm thick tissue slices and embedded in Technovit7100 resin. One to five μm thick sections were cut and observed after staining by either toluidine blue or neutral red. In the X-gal staining positive cells, bluish reaction products were observed around cell nucleus. 2. We observed those blueish reaction products by electron microscopy (EM). Slightly electron dense amorphous materials were identified as reaction products, which enable us to identify lacZ positive cells under EM. 3. Quantum dots worked well as marker for immunocytochemistry of light microscopic level for lacZ, however, they were not effective enough for EM level because of their small size. 4. By experiments in which bone marrow cells of GFP expressing transgenic mice were transplanted to wild type donor mice, we found bone marrow derived cells were distributed in tissues as fibroblast-like cells that connected by gap junctions. A part of c-Kit expressing cells were suggested to be bone marrow derived.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 中村 桂一郎: "ギャップ結合による細胞性ネットワーク-消化管運動ペースメーカー細胞を中心にして-"電子顕微鏡. 37. 188-193 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirata M: "Pigment cell organization in the hypodermis of zebrafish."Dev Dyn. 227. 497-503 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsumoto Y: "Long-term inhibition of Rho-kinase suppresses neointimal formation after stent implantation in porcine coronary arterie : involvment of multiple mechanisms."Arterioscler Thromb Vasc Biol. 24. 181-186 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 中村 桂一郎: "消化管筋層にみられるギャップ結合による細胞性ネットワーク-消化管運動ペースメーカー系-"日薬理誌. 123. 134-140 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] NAKAMURA K: "INTERSTITIAL CELLS OF CAJAL-GAP JUNCTION CONNECTED CELLULAR NETWORKS AS PACEMAKER SYSTEM FOR GASTROINTESINAL MOTILITY."DENSIKENNBIKYOU. 37. 188-193 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] HIRATA M: "PIGMENT CELL ORGANIZATION IN THE HYPODERMI OF ZEBRAFISH."DEV DYN. 277. 497-503 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] MATSUMOTO Y: "LONG-TERM INHIBITION OF RHO-KINASE SUPPRESSES NEOINTIMAL FORMATION AFTER STENT IMPLANTATION IN PORCINE CORONARY ARTERIES : INVOLVMENT OF MULTIPLE MECHANISMS."ARTERIOSCLER THROMB VASC BIOL. 24. 181-186 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] NAKAMURA K: "NETWORK OF PACEMAKER CELLS FOR GASTROINTESTINAL MOTILITY."FOLIA PHARMCOL JPN. 123. 134-140 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirata M: "Pigment cell organization in the hypodermis of zebrafish."Dev Dyn. 227. 497-503 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsumoto Y: "Long-term inhibition of Rho-kinase suppresses neointimal formation after stent implantation in porcine coronary arteries : involvement of multiple mechanisms."Arterioscler Thromb Vasc Biol. 24・1. 181-186 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 中村 桂一郎: "消化管筋層にみられるギャップ結合による細胞性ネットワーク-消化管運動ペースメーカー系-"日薬理誌. 123. 134-140 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 中村桂一郎: "ギャップ結合による細胞性ネットワーク -消化管運動ペースメーカー細胞を中心にして-"電子顕微鏡. 37. 188-193 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shibata Y: "Diversity and molecular anatomy of gap junctions"Med Electron Microsc. 34. 153-159 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kawabuchi M: "The spatiotemporal relationship among Schwann cells, axons and postsynaptic acetylcholine receptor regions during muscle reinnervation in aged rats"Anat Rec. 264. 183-202 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tsutsui H: "Enhanced generation of reactive oxygen species in the limb skeletal muscles from a murine infarct model of heart failure"Circulation. 104. 134-136 (2001)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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