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Cell Biological study on membrane domain formation in glomerular podocyte

Research Project

Project/Area Number 14570029
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionJUNTENDO UNIVERSITY

Principal Investigator

KURIHARA Hidetake  Juntendo Univ., School of Med., Associate Professor, 医学部, 助教授 (80311976)

Co-Investigator(Kenkyū-buntansha) ICHIMURA Koichiro  Juntendo Univ., School of Med., Assistant Professor, 医学部, 助手 (10343485)
SAKAI Tatsuo  Juntendo Univ., School of Med., Professor, 医学部, 教授 (90114488)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsKidney / Glomerulus / Podocyte / Slit diaphragm / Cell-Cell junction / Cell polarity / Cytoskeleton
Research Abstract

In normal kidney, the foot processes of podocytes are maintained wide open to facilitate passage of the glomerular filtrate, and they are held together by the specialized cell-cell contact named slit diaphragms. During glomerular development, the junctional complex containing tight junctions between immature podocytes migrates from the apex to the base of the cells, eventually disappeared, and the junctions are replaced by slit diaphragms suggesting that the slit diaphragm is a modified cell-cell junction. Recently, we have localized another tight junction protein, ASIP/PAR3, which interacts with atypical protein kinase C isotypes and plays a crucial role in the establishment of polarity in various cells. ASIP/PAR3 was detected at the base of slit diaphragms. In developing kidney, the signal for ASIP/PAR3 appeared at the tight junction between podocytes and coincided with that for ZO-1 during differentiation. Adherens junction molecules including cadherins, catenins and afadin disappea … More red during maturation of podocyte. In addition, we have cloned rat podocin, a new member of slit membrane proteins.
The foot processes of podocytes abundantly possess microfilaments. We investigated the actin filament organization of foot processes in adult rat podocytes as well as the formation of actin cytoskeletal system of immature podocytes during glomerulogenesis. Electron microscopy revealed two populations of actin cytoskeleton in foot processes of adult podocytes, one is the actin bundle running above the level of slit diaphragms, the other is the cortical actin network located beneath the plasmalemma. Immunogold labeling for actin-binding proteins demonstrated that alpha-actinin and synaptopodin were localized in the actin bundle, whereas cortactin was in the cortical actin network. We conclude that foot processes have specialized actin filamentous organization and its establishment is associated with the expression and redistribution of actin-binding proteins during development. We also found a novel protein, Vmac, associated with vimentin-type intermediate filaments in podocytes of rat kidney. Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] Shinosaki T et al.: "Glomerular epithelial cell injury accelerates the progression of antibody- induced mesangial proliferative nephritis."Exp Nephrol. 10. 245-258 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinosaki T et al.: "The mechanisms underlying the ameliorative property of lisinopril in progressive mesangioproliferative nephritis."Nephron. 91. 719-729 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinosaki T et al.: "Involvement of DPPIV in immune complex-mediated glomerulonephritis."Lab Invest. 82. 505-513 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirose T et al.: "Involvement of ASIP/PAR-3 in the promotion of epithelial tight junction formation."J Cell Sci. 115. 2485-2495 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ozaki-Kuroda K et al.: "Nectin couples cell-cell adhesion and the actin scaffold at heterotypic testicular junctions."Current Biol. 12. 1145-1150 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hosoyamada Y et al.: "Fibroblasts and antigen-presenting cells in the renal interstitium of streptozotocin-induced diabetic rats on a high cholesterol diet"Arch Histol Cytol. 65. 307-315 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawachi H et al.: "Cloning of rat homologue of podocin : expression in protemuric states and in developing glomeruli."J Am Soc Nephrol. 13. 46-56 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Usui J et al.: "Localization of intercellular adherens junction protein p120 catenin during podocyte differentiation."Anat Embryol. 206. 175-184 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Notoya M et al.: "Intussusceptive capillary growth is required for glomerular repair in rat Thy-1.1 nephritis."Kidney Int. 63. 1365-1373 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asai T et al.: "Pathogenesis of nephrogenic diabetes inspidus by aquaporin-2 C-terminus mutations."Kidney Int. 64. 2-10 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tojo A et al.: "Angiotensin II blockade restores albumin reabsorption in the proximal tubules of diabetic rats."Hypertens Res. 26. 413-419 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ichimura K et al.: "Actin filament organization of foot processes in rat podocytes."J Histochem.Cytochem. 51. 1589-1600 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamamoto Y et al.: "Vmac : a novel protein associated with vimentin-type intermediate filaments in podocytes of rat kidney."Biochim Biophys Res Commun. 315. 1120-1125 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 栗原秀剛: "ポドサイトの分子解剖学"腎と透析. 53(5). 697-701 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 栗原秀剛: "蛋白尿がみられるときに上皮細胞が変形するといわれています。どのような機能、形態上の変化が現れるのですか"小児内科. 36(2). 246-248 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 栗原秀剛: "ジュンケイラ組織学"丸善. 513 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinosaki T et al.: "Glomerular epithelial cell injury accelerates the progression of antibody-induced mesangial proliferative nephritis."Exp Nephrol. 10. 245-258 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinosaki T, Miyai I, Nomura Y, Kobayashi T, Sunagawa N, Kurihara H: "The mechanisms underlying the ameliorative property of lisinopril in progressive mesangioproliferative nephritis."Nephron. 91. 719-729 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shinosaki T, Kobayashi T, Kimura K., Kurihara H: "Involvement of DPPIV in immune complex-mediated glomerulonephritis."Lab Invest. 82. 505-513 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hirose T, Izumi Y, Nagashima Y, Tamai-Nagai Y, Kurihara H, Sakai T, Suzuki Y, Yamanaka T, Suzuki A, Mizuno K, Ohno S: "Involvement of ASIP/PAR-3 in the promotion of epithelial tight junction formation."J Cell Sci. 115. 2485-2495 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ozaki-Kuroda K, Nakanishi H, 115 H, Tanaka H, Kurihara H, Mueller S, Irie K, Ikeda W, Sakai T, Wimmer E, Nishimune Y, Takai Y: "Nectin couples cell-cell adhesion and the actin scaffold at heterotypic testicular junctions."Current Biol. 12. 1145-1150 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hosoyamada Y, Kudo H, Ichimura K, Kurihara H, Sakai T: "Fibroblasts and antigen-presenting cells in the renal interstitium of streptozotocin-induced diabetic rats on a high cholesterol diet."Arch Histol Cytol. 65. 307-315 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawachi H, Koike H, Kurihara H, Sakai T, Shimizu F: "Cloning of rat homologue of podocin: expression in proteinuric states and in developing glomeruli."J Am Soc Nephrol. 13. 46-56 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Usui J, Kurihara H, Shu Y, Tomari S, Kanemoto K, Koyama A, Sakai T, Takahasbi T, Nagata M: "Localization of intercellular adherens junction protein p120 catenin during podocyte differentiation."Anat Embryol. 206. 175-184 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Notoya M, Shinosaki T, Kobayashi T, Sakai T, Kurihara H: "Intussusceptive capillary growth is required for the glomerular repair in rat Thy 1.1. nephritis"Kidney Int. 63. 1365-1373 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asai T, Kuwahara M, Kurihara H, Sakai T, Terada Y, Marumo F, Sasaki S: "Molecular pathogenesis of autosomal dominant nephrogenic diabetes inspidus caused by aquaporin-2 mutations in the c-terminus."Kidney Int. 64. 2-10 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tojo A, Onozato ML, Kurihara H, Sakai T, Goto A, Fujita T: "Angiotensin II blockade restores albumin reabsorption in the proximal tubules of diabetic rats."Hypertens Res. 26. 413-419 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ichimura K, Kurihara H, Sakai T: "Actin filment organization of foot processes in rat podocytes."Hypertens Res. 26. 413-419 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamamoto Y, Irie K, Kurihara H, Sakai T, Takai Y: "Vmac: a novel protein associated with vimentin-type intermediate filaments in podocytes of rat kidney."Biodhim Biophys Res Commun. 315. 1120-1125 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawachi H. et al.: "Cloning of rat homologue of podocin : expression in proteinuric states and in developing glomeruli."J Am Soc Nephrol. 13. 46-56 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Usui J.et al.: "Localization of intercellular adherens junction protein p120 catenin during podocyte differentiation."Anat Embryol. 206. 175-184 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Notoya M. et al.: "Intussusceptive capillary growth is required for glomerular repair in rat Thy-1.1 nephritis."Kidney Int. 63. 1365-1373 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tojo et al.: "Angiotensin II blockade restores albumin reabsorption in the proximal tubules of diabetic rats."Hypertens Res. 26. 413-419 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Asai T.et al.: "Pathogenesis of nephrogenic diabetes inspidus by aquaporin-2 C-terminus mutations."Kidney Int. 64. 2-10 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ichimnura K. et al.: "Actin filament organization of foot processes in rat podocytes."J Histochem Cytochem. 51. 1589-1600 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamamoto Y et al.: "Vmac : a novel protein associated with vimentin-type intermediate filaments in podocytes of rat kidney."Biochem Biophys Res Comm. 315. 1120-1125 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shinosaki T et al.: "Glomerular epithelial cell injury accelerates the progression of antibody-induced mesangial proliferative nephritis"Exp Nephrol. 10. 245-258 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shinosaki T et al.: "The mechanisms underlying the ameliorative property of lisinopril in progressive mesangioproliferative nephritis"Nephron. 91. 719-729 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shinosaki T et al.: "Involvement of DPPIV in immune complex-mediated glomerulonephritis"Lab Invest. 82. 505-513 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirose T et al.: "Involvement of ASIP/PAR-3 in the promotion of epithelial tight junction formation"J Cell Sci. 115. 2485-2495 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ozaki-Kuroda K et al.: "Nectin couples cell-cell adhesion and the actin scaffold at heterotypic testicular junctions"Current Biol. 12. 1145-1150 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hosoyamada Y et al.: "Fibroblasts and antigen-presenting cell in the renal interstitium of streptozotocin-induced diabetic on a high cholesterol diet"Arch Histol Cytol. 65. 307-315 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kawachi H et al.: "Cloning of rat homologue of podocin : expression in proteinuric states and in developing glomeruli"J Am Soc Nephrol. 13. 46-56 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Usui J et al.: "Localization of intercellular adherens junction protein p120 catenin during podocyte differentiation"Anat Embryol. 206. 175-184 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Notoya M et al.: "Intussusceptive capillary growth is required for the glomerular repair in rat Thy 1.1. nephritis"Kidney Int. (In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Asai T et al.: "Molecular pathogenesis of autosomal dominant nephrogenic diabetes inspidus caused by aquaporin-2 mutations in the c-terminus"Kidney Int. (In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 栗原秀剛: "Annual Review 腎臓2002"中外医学社. 275 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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