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The screening of the genes that involved in the determination of the neural area and the neural induction

Research Project

Project/Area Number 14570034
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionRIKEN (The Institute of Physical and Chemical Research)

Principal Investigator

MURAOKA Osamu  RIKEN (The Institute of Physical and Chemical Research), Laboratory for vertebrate axis formation, Research scientist, 体軸形成研究チーム, 研究員 (20283765)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordszebrafish / neural area / kheper / mouse / ES cell / Expression cloning / Zebrafish / ES cell / Mouse / Expression cloning / ゼブラフイツシュ / 神経発生
Research Abstract

We screened cDNA libraries using expression screen strategy to identify the factors that regulated the development of the neuroectoderm. We made the plasmid cDNA libraries from zebrafish embryos, mouse embryos, and early differentiated mouse embryonic stem (ES) cells. These were transformed into the E.coli and divided in groups which contained 200 clones each. We prepared the plasmid from each groups and made RNAs for the microinjection. These RNAs were injected into the zebrafish embryos at one to two cell stage. We checked the phenotype by microscopic view and the whole mount in situ hybridization with neural marker probes. As the neural marker, we used six3,eng3,krox20 and kheper. kheper is the new transcription factor which we cloned. kheper is expressed in the whole neuroectoderm and regulated the development of the neural tissue of zebrafish.
We screened 23 x 10^4 clones and found many well known genes. In addition, we succeeded to identify new FGF family gene and new Bcl2 family gene and new wnt family gene. When these are overexpressed in zebrafish embryos, the neuroectoderm were enlarged. The expression of the new fgf was detected in the anterior region of the central nervous system. It is suggested that the new FGF regulates the development of the forebrain.
We are going to do the epistatic analysis of these factors. We would like to know the relationship of these with kheper and how they regulate the development of neural tissue.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Bae YK, Shimizu T, Yabe T, Kim CH, Hirata T, Nojima H, Muraoka O, Hirano T, Hibi M.: "A homeobox gene, pnx, is involved in the formation of posterior neurons in zebrafish"Development. 130. 1853-1865 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yabe T, Shimizu T, Muraoka O, Bae YK, Hirata T, Nojima H, Kawakami A, Hirano T, Hibi M.: "Ogon/Secreted Frizzled functions as a negative feedback regulator of Bmp signaling"Development. 130. 2705-2716 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideaki Nojima, Takashi Shimizu, Cheol-Hee Kim, Taijiro Yabe, Young-Ki Bae, Osamu Muraoka, Tsutomu Hirata, Ajay Chitnis, Toshio Hirano, Masahiko Hibi: "Genetic evidence for involvement of maternally derived Wnt canonical signaling in dorsal determination in zebrafish"Mechanisms of Development. 121. 371-386 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hisashi Hashimoto, Michael Rebagliati, Nadira Ahmad, Osamu Muraoka, Tadahide Kurokawa, Masahiko Hibi, Tohru Suzuki: "The Cerberus/Dan-family protein Charon is a negative regulator of Nodal signaling during left-right patterning in zebrafish"Development. 131. 1741-1753 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Bae YK, Shimizu T, Yabe T, Kim CH, Hirata T, Nojima H, Muraoka O, Hirano T, Hibi M.: "A homeobox gene, pnx, is involved in the formation of posterior neurons in zebrafish."Development.. 130. 1853-1865 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yabe T, Shimizu T, Muraoka O, Bae YK, Hirata T, Nojima H, Kawakami A, Hirano T, Hibi M.: "Ogon/Secreted. Frizzled functions as a negative feedback regulator of Bmp signaling."Development.. 130. 2705-2716 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hashimoto H, Rebagliati M, Ahmad N, Muraoka O, Kurokawa T, Hibi M, Suzuki T.: "The Cerberus/Dan-family protein Charon is a negative regulator of Nodal signaling during left-right patterning in zebrafish"Development.. 131. 1741-1753 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nojima H, Shimizu T, Kim CH, Yabe T, Bae YK, Muraoka O, Hirata T, Chitnis A, Hirano T, Hibi M.: "Genetic evidence for involvement of maternally derived Wnt canonical signaling in dorsal determination in zebrafish."Mech Dev.. 121. 371-386 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yabe T, Shimizu T, Muraok O, Bae YK, Hirata T, Nojima H, Kawakami A, Hirano T, Hibi M.: "Ogon/Secreted Frizzled functions as a negative feedback regulator of Bmp signaling."Development. 130. 2705-2716 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Bae YK, Shimizu T, Yabe T, Kim CH, Hirata T, Nojima H, Muraoka O, Hirano T, Hibi M.: "A homeobox gene, pnx, is involoved in the formation of posterior neurons in zebrafish"Development. 130. 1853-1865 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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