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Development of myosin isoform function in single ventricular myocytesHeart

Research Project

Project/Area Number 14570046
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionTeikyo University

Principal Investigator

SUGI Haruo  Teikyo University, School of Medicine, Professor, 医学部, 教授 (20082076)

Co-Investigator(Kenkyū-buntansha) YAMASHITA Hiroshi  University of Tokyo, Department of Medicine, Instructor, 医学部, 助手 (50323572)
KOBAYASHI Takakazu  Shibaura Institute of Technology, Department of Technology, Professor, 工学部, 教授 (00112756)
SUGIURA Seiryo  University of Tokyo, Graduate school of Frontier Science, Professor, 新領域創生科学研究科, 教授 (10272551)
SHIRAKAWA Ibuki  Teikyo University, School of Medicine, Instructor, 医学部, 助手 (80236190)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsHeart / Ventricular myocyte / Myosin isoform / Muscle contraction / Laser optical trap / ATP / Ca^<2+>過渡現象 / レーザーオプティカルとラップ
Research Abstract

Rat ventricle normally contains a-rnyosin heavy chain isoforms, and if exposed to ventricular overload, they are replaced by n-heavy chain isoforms, to result in a decrease in tension cost. Human ventricle, on the other hand, only consists of β-myosin heavy chain isoforms, and the isoform composition does not change under the condition of ventricular overload.
To given information about the mode of adaptation of human ventricle to overload, we studied the effect of changing the myosin light chain isoforms on the motor function of ventricular myosin. We found that myosin light chain isoforms actually affects the magnitude of force exerted by each ventricular myosin molecule.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Yamashita, H., Sugiura, S., Fujita, H., Yasuda, S., Nagai, R., Saeki, Y., Sunagawa, K., Sugi, H.: "Myosin light chain isoforms modify force-generating ability of cardiac myosin by changing the kinetics of actin-myosin interaction."Cardiovascular Research. 60・3. 580-588 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda, S., Sugiura, S., Yamashita, H., Nishimura, S..Saeki, Y., Momomura, S., Katoh, K., Nagai, R., Sugi, H.: "Unloaded shortening increases peak of Ca^<2+> transients but accelerates their decay in rat single cardiac myocytes."American Journal of Physiology - Heart and Circulatory Physiology. 285. H470-H475 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sugi, H., Iwamoto, H., Akimoto, T., Kishi, H: "High mechanical efficiency of the cross-bridge powerstroke in skeletal muscle"The Journal of Experimental Biology. 206. 1201-1206 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi, I., Shimada, M., Akimoto, T., Kishi, H., Sugi, H.: "Electron microscopic evidence for the thick filament interconnections associated with the catch state in the anterior byssal retractor muscle of Mytilus edulis"Comparative Biochemistry and Physiology. A134. 115-120 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki, S., Nagayoshi, H., Ishino, K., Hino, N., Sugi, H.: "Ultrastructural organization of the transverse tubules and the sarcoplasmic reticulum in a fish sound-producinf muscle."Journal of Electron Microscopy. 52. 337-347 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sugi, H., Chaen, S.: "Force-velocity relationships in actin-myosin interactions causing cytoplasmic streaming in algal cells"The Journal of Experimental Biology. 206. 1971-1976 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamashita, H., Sugiura, S., Fujita, H., Yasuda, S., Nagai, R., Saeki, Y., Sunagawa, K., Sugi, H.: "Myosin light chain isoforms modify force-generating ability of cardiac myosin by changing the kinetics of actin-myosin interaction"Cardiovascular Research. 60(3). 580-588 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuda, S., Sugiura, S., Yamashita, H., Nishimura, S., Saeki, Y., Momomura, S., Katoh, K., Nagai, R., Sugi, H.: "Unloaded shortening increases peak of Ca^<2+> but accelerates their decay in rat single cardiac myocytes"American Journal of Physiology -Heart and Circulatory Physiology. 285. H470-H475 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sugi, H., Iwamoto, H., Akimoto, T., Kishi, H.: "High mechanical efficiency of the cross-bridge powersiroke in skeletal muscle"The Journal of Experimental Biology. 206. 1201-1206 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi, I., Shimada, M., Akimoto, T., Kishi, H., Sugi, H.: "Electron microscopic evidence for the thick filament interconnections associated with the catch state in the anterior byssal retractor muscle of Myrilus edulis"Comparative Biochemistry and Physiology. A134. 115-120 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki, S., Nagayoshi, H., Ishino, K., Hino, N., Sugi, H.: "Ultrastnictural organization of the transverse tubules and the sarcoplasmic reticulum in a fish sound-producinf muscle"Journal of Electron Microscopy. 52. 337-347 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sugi, H., Chaen, S.: "Force-velocity relationships in actin-myosin interactions causing cytoplasmic streaming in algal cells"The Journal of Experimental Biology. 206. 1971-1976 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamashita, H., Sugiura, S., Fujita, H., Yasuda, S., Nagai, R., Saeki, Y., Sunagawa, K., Sugi, H.: "Myosin light chain isoforms modify force-generating ability of cardiac myosin by changing the kinetics of actin-myosin interaction."Cardiovascular Research. 60・3. 580-588 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yasuda, S., Sugiura, S., Yamashita, H., Nishimura, S., Saeki, Y., Momomura, S., Katoh, K., Nagai, R., Sugi, H.: "Unloaded shortening increases peak of Ca^<2+> transients but accelerates their decay in rat single cardiac myocytes."American Journal of Physiology - Heart and Circulatory Physiology. 285. H470-H475 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sugi, H., Iwamoto, H., Akimoto, T., Kishi, H: "High mechanical efficiency of the cross-bridge powerstroke in skeletal muscle"The Journal of Experimental Biology. 206. 1201-1206 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takahashi, I., Shimada, M., Akimoto, T., Kishi, H., Sugi, H.: "Electron microscopic evidence for the thick filament interconnections associated with the catch state in the anterior byssal retractor muscle of Mytilus edulis"Comparative Biochemistry and Physiology. A134. 115-120 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki, S., Nagayoshi, H., Ishino, K., Hino, N., Sugi, H.: "Ultrastructural organization of the transverse tubules and the sarcoplasmic reticulum in a fish sound-producinf muscle."Journal of Electron Microscopy. 52. 337-347 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sugi, H., Chaen, S.: "Force-velocity relationships in actin-myosin interactions causing cytoplasmic streaming in algal cells"The Journal of Experimental Biology. 206. 1971-1976 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takahashi, I., Shimada, M., Akimoto, T., Kishi, T., Sugi, H.: "Electronmicroscopic evidence for the thick filament interconnections associated with the catch state in the anterior byssal retractor muscle of Mytilus edulis"Comparative Biochemistry and Physiology. A134. 115-120 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sugi, H., Iwamoto, H., Akimoto, T., Kishi, H.: "High mechanical efficiency of the cross-bridge powerstroke in skeletal muscle"Journal of Experimental Biology. 206. 1201-1206 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sugi, H., Chaen, S.: "Force-velocity relationships in actin-myosin interactions causing cytoplasmic streaming in algal cells"Journal of Experimental Biology. 206(in press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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