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Study with knockout mice on the activation mechanisms of the capacitative Ca^<2+> entry channel existing in the cardiac myocyte

Research Project

Project/Area Number 14570051
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionFukuoka University

Principal Investigator

UEHARA Akira  Fukuoka University, School of Medicine, Associate Professor, 医学部, 助教授 (60140745)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsHeart / Calcium / Sarcoplasmic reticulum
Research Abstract

We have explored whether the capacitative Ca^<2+> entry (CCE) mechanisms exists in heart muscle with a fluorescent [Ca]_i measurement technique. By the reintroduction of external Ca^<2+> after the depletion of Ca^<2+> stored in sarcoplasmic reticulum (SR) using Ca^<2+> pump inhibitors or ryanodine receptor (RyR) activators, a huge steady rise in cytoplasmic Ca^<2+> concentration at both tissue and single cell levels was evoked even in the presence of L-type Ca^<2+> channel antagonists. Such a CCE activity was sensitive to Zinc but insensitive to the other many cation blockers in possible molecular entities of CCE channels of TRP and Icrac channel families. Here we could thus first demonstrate from embryonic to neonatal stages at least that the PL membrane of the cardiac myocyte is endowed with a store-operated Ca^<2+> entry pathway. Cardiac SR could be refilled with Ca^<2+> via the CCE pathway and reset as a functional regulator of the cytoplasmic Ca^<2+> concentration to induce the multiple Ca^<2+> dependent cellular function, whenever the stored Ca^<2+> in the SR lumen is consumed and subsequently depleted during ontogenetic developing stages or under some physiological and pathophysiological conditions.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Uehara, A.et al.: "Store-operated Ca^<2+> entry uncoupled with ryanodine receptor and junctional membrane complex in heart muscle"Cell Calcium. 31. 89-96 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uehara, A., Yasukochi, M., Imanaga, I., Nishi, M., Takeshima, H.: "Store-operated Ca^<2+> entry uncoupled with ryanodine receptor and junctional membrane complex in heart muscle"Cell Calcium. 31. 89-96 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uehara, A., Yasukochi, M., Imanaga, I., Nishi, M., Takeshima, H.: "Store-operated Ca^<2+> entry uncoupled with ryanodine receptor and junctional membrane complex in heart muscle"Cell Calcium. 31. 89-96 (2002)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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