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Ontogeny of unique circadian rhythm in rhythm mutant mice

Research Project

Project/Area Number 14570054
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

ABE Hiroshi  Hokkaido Univ., Grad.School of Med., Lec., 大学院・医学研究科, 講師 (80201896)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordscircadian rhythm / ontogeny / rhythm splitting / clock genes / suprachiasmatic nucleus / behavior / histamine / brain / スプリッティング / ミュータントマウス / CS系マウス
Research Abstract

CS mice show spontaneous splitting in the circadian rhythm of behavior under continuous darkness. To clarify ontogeny of rhythm splitting in the CS mice, the behavioral rhythm and clock gene rhythm in the brain of CS adult and pups were examined. In the behavioral rhythm, P21 pups showed splitting pattern under LD cycle. CS pups, which were reared by foster mother of C57BL/6J after birth, also showed clear split pattern. In the clock gene rhythm, Per1 and Per2 mRNA rhythms in the SCN and other brain areas of CS P13 and P21 pups were examined by in situ hybridization. Data are currently being analyzed.
To compare the ontogeny of CS's rhythm with that of rats, ontogeny of clock gene rhythm in the SCN of rats were examined. The results showed that Per1 and Per2 rhythms of P6 pups were influenced by mother's rhythm, and that the phases of the pup rhythms were shifted by periodic absence of nursing mother for the first week after birth.
To clarify the functional role of brain histamine in the circadian clock system, the behavioral rhythm and clock gene rhythm in the brain of histidine decarboxylase deficient mice (HDC mice) were examined. In the behavioral rhythm, HDC mice showed low activity level under LD and DD, and prolonged freerunning period under DD. In the clock gene rhythm, there was no difference in the Per1 and Per2 rhythms in the SCN of HDC mice from that of wild type mice. However, the Per1 and Per2 in the other brain areas such as cerebral cortex and striatum showed no rhythm or damped oscillation. These results suggest that brain histamine is involved in the output mechanism from the SCN pacemaker.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Abe, H.: "Circadian rhythms in behavior and clock gene expressions in the brain of mice lacking histidine decarboxylase."Molecular Brain Research. 124. 178-187 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohta, H.: "Periodic absence of nursing mothers alters circadian clock in newborn rats."European Journal of Neuroscience. 17. 1628-1634 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishizaki, K.: "Gene expression of neuropeptide Y in the nucleus of the solitary tract is activated in rats under restricted daily feeding but not under 48th food deprivation."European Journal of Neuroscience. 17. 2097-2105 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohta, H.: "Effects of nursing mothers on rPer1/rPer2 circadian expressions in the neonatal suprachiasmatic nuclei vary with developmental stage."European Journal of Neuroscience. 15. 1953-1960 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 安倍 博: "時計遺伝子によるリズム発振:主時計と末梢時計の脱同調"臨床脳波. 46. 229-236 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 安倍 博: "催眠・覚醒の分子生物学的制御"現代医療. 35. 34-40 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 安倍 博: "体内時計関連遺伝子の働き方"BRAIN MEDICAL. 15. 133-141 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Abe, H.: "Circadian rhythms in behavior and clock gene expressions in the brain of mice lacking histidine decarboxylase."Molecular Brain Research. 124. 178-187 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohta, H.: "Periodic absence of nursing mothers alters circadian clock in newborn rats."European Journal of Neuroscience. 17. 1628-1634 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishizaki, K.: "Gene expression of neuropeptide Y in the nucleus of the solitary tract is activated in rats under restricted daily feeding but not under 48h food deprivation."European Journal of Neuroscience. 17. 2097-2105 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohta, H.: "Effects of nursing mothers on rPer1/rPer2 circadian expressions in the neonatal suprachiasmatic nuclei vary with developmental stage."European Journal of Neuroscience. 15. 1953-1960 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Abe, H.: "Molecular mechanism of circadian clock : dissociation between SCN main clock and peripheral clocks.(Japanese)"Clinical Electroencephalography. 46. 229-236 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Abe, H.: "Molecular control for circadian sleep-wake rhythm.(Japanese)"New Horizon for Medicine. 35. 34-40 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Abe, H.: "The function of clock relating genes in circadian system.(Japanese)"BRAIN MEDICAL. 15. 133-141 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Abe, H.: "Circadian rhythms in behavior and clock gene expressions in the brain of mice lacking histidine decarboxylase"Molecular Brain Research. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohta, H.: "Periodic absence of nursing mothers alters circadian clock in newborn rats"European Journal of Neuroscience. 17. 1628-1634 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ishizaki, K.: "Gene expression of neuropeptide Y in the nucleus of the solitary tract is activated in rats under restricted daily feeding but not under 48h food deprivation"European Journal of Neuroscience. 17. 2097-2105 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 安倍 博: "時計遺伝子によるリズム発振:主時計と末梢時計の脱同調"臨床脳波. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 安倍 博: "睡眠・覚醒の分子生物学的制御"現代医療. 35. 34-40 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 安倍 博: "体内時計関連遺伝子の働き方"BRAIN MEDICAL. 15. 133-141 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohta, H.: "Effects of nursing mothers on rPer1/ePer2 circadian expressions in the neonatal suprachiasmatic nuclei vary with development stage"European Journal of Neuroscience. 15. 1953-1960 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohta, H.: "Periodic absence of nursing mothers alters circadian clock in newborn rats"European Journal of Neuroscience. (in press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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