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Effects of environmental temperature on physiological function of the brain of the rats suffered hypoxic-ischemic brain insult during neonatal period.

Research Project

Project/Area Number 14570057
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionOsaka Electro-Communication University

Principal Investigator

HOSONO Takayoshi  Osaka Electro-Communication University, Faculty of Engineering, Professor, 工学部, 教授 (60294104)

Co-Investigator(Kenkyū-buntansha) FUKUDA Hirotsugu  Osaka University, Graduate School of Medicine, Assistant Professor, 大学院・医学系研究科, 助手 (40324751)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsbrain damage / newborn / environmental temberature / rat / thermoregulation / optic recording / auditory brainstem response / 脳性脳幹反応 / 低酸素
Research Abstract

In order to investigate the effect of brain cooling of the neonate suffered hypoxic-ischemic brain-damage, we performed experiments using animal model. Under the inspired anesthesia, we ligated left common carotid artery of the 7-day-old rat neonate, and exposed them to the hypoxic air (oxygen 8%). After the hypoxic exposure, we divided the rat neonates to three groups. The first group (hypothermia group) was kept in a room of low environmental temperature for 24 hr, which led the rats to the hypothermia (30 degree). The second group (normothermia group) was kept in a room of normal temperature for 24 hr, which kept the rat body temperature in normothermia. The third group was a control, which had a sham operation. After rearing for three months, we performed auditory brainstem response (ABR) for three groups. ABR was successfully recorded in all the rats under light anesthesia. The interpeak latency of III and V peaks of the normothermia group was significantly elongated than those of the hypothermia and control group. The ratio of the amplitude of V peak versus I peak of the normothermia group was also significantly small than those of the other groups. The inferior colliculus of the normothermia group was significantly atrophic than those of the other groups by using light microscope and imaging-data processing software. These findings indicate that high environmental temperature for the neonate rats after the hypoxic-ischemic insult caused more damages in rat midbrain function than low environmental temperature does. We also performed preliminary investigation on the function of neuronal circuit using optic recording, and found that optic recording using low perfusion temperature might be a useful investigation tools for the study of damaged brain function of suffered neonate animal models.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Hosono T, Nakano M, Terai R, Yagishita T, Okuno R, et al.: "Analysis of signal transmissions from suprachiasmatic nucleus in the rat brain using optic recording"Trans Jpn Soc Med Biol Engng. 41. 405-409 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomimatsu T, Fukuda H, Endoh M, Mu J, Kanagawa T, Hosono T, et al.: "Long-Term Neuroprotective Effects of Hypothermia on Neonatal Hypoxic-Ischemic Brain Injury in Rats, Assessed by Auditory Brainstem Response"Pediatric Res. 53. 57-61 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuda H, Tomimatsu T, Kanagawa T, Mu J, Hosono T, et al.: "Postischemic Hyperthermia Induced Caspase-3 Activation in the Newborn Rat Brain after Hypoxia-Ischemia and Exacerbated the Brain Damage"Biol Neonat. 84. 164-171 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshida K, Maruyama M, Hosono T, Nagashima K, et al.: "Fos expression induced by warming the preoptic area in rats."Brain Res. 933. 109-117 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hosono T, Nakano M, Terai R, Yagishita T, Okuno R, Akazawa K, Kanosue K.: "Analysis of signal transmissions from suprachiasmatic nucleus in the rat brain using optic recording."Trans Jpn Soc Med Biol Engng. 41(4). 405-409 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomimatsu T, Fukuda H, Endoh M, Nu J, Kanagawa T, Hosono T, Kanzaki T, Doi K, Kubo T, Murata Y.: "Long-term neuroprotective effects of hypothermia on neonatal hypoxic-ischemic brain injury in rats, assessed by auditory response."Pediatric Res. 53(1). 57-61 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuda H, Tomimatsu T, Kanagawa T, Mu J, Kohzuki M, Shimoya K, Hosono T, Kanzaki T, Murata Y.: "Postisohemic hyperthermia induced caspase-3 activation in the newborn rat brain after hypoxia-ischemia and exacerbated the brain damage."Biol Neonat. 84(2). 164-171 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshida K, Maruyama M, Hosono T, Nagashima K, Fukuda Y, Gerstberger R, Kanosue K.: "Fos expression induced by warming the preoptic area in rats."Brain Res. 933(2). 109-117 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuda H, Tomimatsu T, Kanagawa T, Mu J, Kohzuki M, Shimoya K, Hosono T, et al.: "Postischemic hyperthermia induced caspase-3 activation in the newborn rat brain after hypoxia-ischemia and exacerbated the brain damage"Biology of the Neonate. 84(2). 164-171 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hosono T, Nakano M, Terai R, Yagishita T, Okuno R, Akazawa K, Kanosue K: "Analysis of signal transmissions from suprachiasmatic nucleus in the rat brain using optical recording."Transactions of the Japanese Society for Medical and Biological Engineering.. 41(4). 405-409 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshida K, Maruyama M, Hosono T, Nagashima K, Fukuda Y, et al.: "Fos expression induced by warming the preoptic area in rats"Brain Research. 933. 109-117 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tomimatsu T, Fukuda H, Endoh M, Junwu Mu, Kanagawa T, Hosono T, et al.: "Long-term neuroprotective effects of hypothermia on neuronal hypoxic-ischemic brain injury in rats, assessed by auditory brainstem response"Pediatric Research. 53. 57-61 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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