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Studies of molecular Nutrition on Retinoid absorption and metabolism

Research Project

Project/Area Number 14570066
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionSiebold University of Nagasaki

Principal Investigator

TAKASE Sachiko  Siebold University of Nagasaki, Dept. Nutrition, Professor, 看護栄養学部, 教授 (10046196)

Co-Investigator(Kenkyū-buntansha) GODA Tohinao  University of Shizuoka, Dep. Food & Nutrition Sciences, Associate Professor, 食品栄養科学部, 教授 (70195923)
YAMAMOTO Takeshi  Siebold University of Nagasaki, Dep. Nutrition, instructor, 助手 (70316181)
SHIDOJI Yoshihiro  Siebold University of Nagasaki, Dep. Nutrition, Professor, 教授 (00111518)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsCRBPII / PPARS / coactivator (p300) / β-carotene dioxygenase / chick duodenum / Hydrocorticoid / rat jejunum / thyroid hormone (T3) / CRBP II / 発達過程 / レチノール吸収 / PPARα / PPARδ / coactivator / CBP / p300 / rat intestine
Research Abstract

1. Our investigations revealed that gene expression levels of cellular retinol-binding protein type II (CRBPII) is modulated through postnatal development of PPAR subtypes and endogenous transcriptional activator p300. This p300 coactivator is interacted with PPARa or PPAR6 in the presence of their specific ligands such as unsaturated fatty acids. During the weaning period (17day -25day ages) the PPARa/b ratio increased and CRBPII mRNA strongly expressed.
2. Developmental expression pattern, of frcarotene cleavage enzyme (BCCE) activities are increased during the postnal development of small intestine of rats as well as chicks. The BCCE activity in rat jejunum was elevated during the weaning period. In chick case, duodenal BCCE activity was induced after their birth with its peak activity at 5 days after their hatching. The BCCE activity in rat jejunum was elevated by hormones of hydrocortisone (glucocorticoid) as well as thyroid hormone T3. The weaning diets containing high or low diet … More ary fat affected its activity showing that the high fat-feeding induced low BCCE activity and the low fat-feeding induced high BCCE activity in weaning rats. This results may explain why mother milk feeding during suckring period induce lower activity of BCCE in rat jejunum and why higher activity after weaning during rat development.
3. After hatching, chick duodenum expressed highly BCCE mRNA, which levels increased developmentally and its peak expression occurred at 5 days after thir birth. The administration of hydrocortisone to chick embryo on day 14 and 17 of incubation, developmental profile of BCCE mRNA level changed to early its induction. The results suggested that endogeneous hormone level might involed to in the developmental induction of intestinal BCCE gene expression.
4. It is clalified that dietary high fat induced CRBPII gene expression are accompanied with increases in intestinal retinol absorption and liver uptake of retinol delivered from intestine as well as chromicrone synthesis by using a indicator of microsomal transfer protein mRNA expression. Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Mochizuki K, Suruga K, Sakaguchi N, Takase S, Goda T: "Major intestinal coactivator p300 strongly activates peroxisome proliferator-activated receptor in intestinal cell line, Caco-2."GENE. 291. 271-277 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ogura Y, Suruga K, Mochizuki H, Yamamoto T, Takase S, Goda T: "Postnatal changes in gene expression of retinal dehydrogenase and retinoid receptors in liver of rats."Life Sciences. 74. 1519-1528 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 合田敏尚, 駿河和仁, 高瀬幸子: "ビタミンAの腸管吸収"細胞. 34. 88-91 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 高瀬幸子, 四童子好廣, 山本武: "小腸におけるβカロテン代謝調節のメカニズム究明"平成13年度シーボルト大学共同教育研究報告書. 227-230 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 高瀬幸子 他(分担執筆): "ビタミン(日本ビタミン学会編)"ビタミン:研究のブレークスルー,学進出版. 21-24 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mochizuki K, Suruga K, Sakaguchi N, Takase S, Goda T.: "Major intestinal coactivator p300 strongly activates peroxisome proliferator-activated receptor in intestinal cell line, Caeo-2."GENE. 291. 271-277 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ogura Y, Suruga K, Mochizuki H, Yamamoto T, Takase S, Goda T: "Postnatal changes in gene expression of retinal dehydrogenase and retinoid receptors in liver of rats."Life Sciences. 74. 1519-1528 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 合田敏尚, 駿河和仁, 高瀬幸子: "ビタミンAの腸管吸収"細胞. 34(3). 88-91 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 高瀬幸子, 四童子好廣, 山本武: "小腸におけるβカロテン代謝調節のメカニズム究明"平成13年度シーボルト大学協同教育研究報告書. 227-230 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 高瀬幸子 他(分担執筆): "プロビタミンAとしての発見と研究,第2章カロテノイド"(日本ビタミン学会編)ビタミン:研究のブレークスルー(学進出版). 21-24 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ogura Y et al.: "Postnatal changes in gene expression of retinal dehydrogenase and retinoid receptors in liver of rats."Life Science. 74(12). 1519-1528 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 高瀬 幸子 他: "小腸におけるβカロテン代謝調節のメカニズム究明"県立長崎シーボルト大学共同教育研究に係る研究報告書. 227-230 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mochiuki K et al.: "Major intestinal coactivator p300 strongly activates peroxisome proliferator-activated receptor in intestinal cell line, Caco-2"Gene.. 292(1-2). 271-277 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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