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IN SEARCH OF THE COUSE AND TREAMENT FOR JETLAG

Research Project

Project/Area Number 14570071
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionKINKI UNIVERSITY

Principal Investigator

SHIGEYOSHI Yasufumi  KINKI UNIVERSITY, SCHOOL O FMEDICINE, PROFESSOR, 医学部, 教授 (20275192)

Co-Investigator(Kenkyū-buntansha) ADACHI Akihito  KINKI UNIVERSITY, SCHOOL O FMEDICINE, ASSISTANT, 医学部, 助手 (20351588)
NAGANO Mamoru  KINKI UNIVERSITY, SCHOOL O FMEDICINE, ASSISTANT, 医学部, 助手 (80155960)
FUJIOKA Atsuko  KINKI UNIVERSITY, SCHOOL O FMEDICINE, ASSISTANT PROFESSOR, 医学部, 助教授 (30077664)
佐藤 伸介  近畿大学, 医学部, 助手 (40270574)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsSUPRACHIASMATIC NUCLEUS / DESYNCHRONY / ETLAG / JETLAG SYNDROME / CIRCADIAN RHYTHM / GENEME / Per1 / INTTERNAL CLOCK / Perl / Per2 / Cry1 / Rev-erb / Gene Chip
Research Abstract

1.Dichotomy of the circadian center is associated with jet lag syndrome
Dissociation of the suprachiasmatic nucleus, the circadian center, into two oscillators was observed after 10 hour delay and 6 hour advance of light-dark cycle. The ventrolateral region of the SCN(VLSCN) shifted rapidly whereas the dorsomedial regions of the SCN(DMSCN) shifted slowly, spent more than a week to regain synchronization of the VLSCN and DMSCN. The sluggish shift of the DMSCN corresponded to the suppressed locomotors activity during the subjective night, which suggested that jet lag was caused by the slow shift of the DMSCN after LD cycle shift.
2.A Transcription Factor Response Element for Gene Expression During Circadian Night
We profiled suprachiasmatic nuclei(SCN) and liver genome-wide expression patterns under light/dark(LD) cycles and constant darkness(DD). We determined transcription start sites(TSS) of human orthologues for newly identified cycling genes and then performed bioinformatical searches for relationships between time-of-day specific expression and transcription factor response elements around TSS. We demonstrate the role of the Rev-ErbA/ROR response element in gene expression of nocturnally expressed genes in SCN and liver.
3.Phosphodiesterase type 4(PDE4) specifically degrade cAMP
To elongate Per1 expression by the inhibition of cAMP degradation, we used Rolipram, a specific inhibitor of phosphodiesterase type 4, which degrades the cAMP. With Rolipram application, the amount of Perl transcripts increased transiently but the period length of Perl induction was not extended.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] T.Iwasaki: "A partial hepatectomy results in altered expression of clock-related and cyclic glyceraldehyde 3-phosphate dehydrogenase(GAPDH)genes."Life Sciences. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Kawamoto: "Two types of VIP neuronal components in rat suprachiasmatic nucleus."J.Neurosci.. 74. 852-857 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Nagano: "An abrupt shift in the day : night cycle causes desynchrony in the mammalian circadian center."J.Neurosci. 23. 6141-6151 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.H.Masumoto: "Effect of phosphodiesterase type 4 on circadian clock gene Per1 transcription."Biochem Biophys Res Commnun. 306. 781-785 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Sato: "Inhibition of rostral basal forebrain neurons promotes wakefulness and induces FOS in orexin neurons."Eur J.Neurosci. 17. 1635-1645 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ueda: "Transcription Factor Response Element for Gene Expression During Circadian Night."Nature. 418. 534-539 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Nakahama: "A role of the C-terminus of aquaporin 4 in its membrane expression in cultured astrocytes."Genes Cells. 7. 731-741 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Shigeyoshi: "Restoration of circadian behavioral rhythms in a period null Drosophila mutant (per01)by mammalian period homologues mPerl and mPer2"Genes Cells. 72. 163-171 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ken-ichi Honma: "Circadian clock as multi-oscillation system"Hokkaido University Press Sapporo. 244 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Iwasaki: "A partial hepatectomy results in aliened expression of clock-related and cyclic glyceraldehyde 3-phosphate dehydrogenase(GAPDH) genes."Life Sciences. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Kawamoto: "Two types of VIP neuronal components in rat suprachiasmatic nucleus"J.Neurosci. 74. 852-857 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Nagano: "An abrupt shift in the day : night cycle causes desynchrony in the mammalian circadian center."J.Neurosci. 23. 6141-6151 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.H.Masumoto: "Effect of phosphodiesterase type 4 on circadian clock gene Per1 transcription."Biochem Biophys Res Commnun. 306. 781-785 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Satoh: "Inhibition of rostral basal forebrain neurons promotes wakefulness and induces FOS in orexin neurons."Eur J.Neurosci. 17. 1635-1645 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ueda: "Transcription Factor Response Element for Gene Expression During Circadian Night."Nature. 418. 534-539 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Nakahama: "A role of the C-terminus of aquaporin 4 in its membrane expression in cultured astrocytes."Genes Cells. 7. 731-741 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Shigeyoshi: "Restoration of circadian behavioral rhythms in a period null Drosophilamut ant(perO1) by mammalian period homologues mPer1 and mPer2."Genes Cells. 7. 163-171 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Iwasaki: "A partial hepatectomy results in altered expression of clock-related and cyclic glyceraldehyde 3-phosphate dehydrogenase(GAPDH)genes."Life Sciences. (In press).

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Kawamoto: "Two types of VIP neuronal components in rat suprachiasmatic nucleus."J. Neurosci.. 74. 852-857 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Nagano: "An abrupt shift in the day : night cycle causes desynchrony in the mammalian circadian center."J. Neurosci. 23. 6141-6151 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.H.Masumoto: "Effect of phosphodiesterase type 4 on circadian clock gene Per1 transcription."Biochem Biophys Res Commnun. 306. 781-785 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Satoh: "Inhibition of rostral basal forebrain neurons promotes wakefulness and induces FOS in orexin neurons."Eur J. Neurosci. 17. 1635-1645 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Ueda: "Transcription Factor Response Element for Gene Expression During Circadian Night."Nature. 418. 534-539 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Nakahama: "A role of the C-terminus of aquaporin 4 in its membrane expression in cultured astrocytes."Genes Cells. 7. 731-741 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Shigeyoshi: "Restoration of circadian behavioral rhythms in a period null Drosophila mutant(per01)by mammalian period homologues mPer1 and mPer2"Genes Cells. 72. 163-171 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ken-ichi Honma: "Circadian clock as multi-oscillation system"Hokkaido University Press Sapporo. 244 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shigeyoshi Y, Meyer-Bernstein E, Yagita K, Fu W, Chen Y, Takumi T, Schotland P, Sehgal A, Okamura H.: "Restoration of circadian behavioural rhythms in a period null Drosophila mutant (per01) by manunalian period homologues mPer1 and mPer2"Genes Cells. 7(2). 163-171 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakahama K, Fujioka A, Nagano M, Satoh S, Furukawa K, Sasaki H, Shigeyoshi Y.: "A role of the C-terminus of aquaporin 4 in its membrane expression in cultured astrocytes"Genes Cells. 7(7). 731-741 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ueda HR, Chen W, Adachi A, Wakamatsu H, Hayashi S, Takasugi T, Nagano M, Nakahama K, Suzuki Y, Sugano S, Iino M. Shigeyoshi Y, Hashimoto S.: "A transcription factor response element for gene expression during circadian night"Nature. 418(68979). 534-539 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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