• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effects of adenosine receptor stimulation on guinea pig cardiac myocytes

Research Project

Project/Area Number 14570091
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionKANAZAWA MEDICAL UNIVERSITY

Principal Investigator

NISHIO Matomo  KANAZAWA MEDICAL UNIVERSITY, Medical school, Professor, 医学部, 教授 (80156041)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Junko  KANAZAWA MEDICAL UNIVERSITY, Medical school, Lecturer, 医学部, 講師 (20064628)
ISHIBASHI Takaharu  KANAZAWA MEDICAL UNIVERSITY, Medical school, Associate Professor, 医学部, 助教授 (60184561)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsadenosine / cardiomyocyte / A_1 receptor / A_1 receptor antagonist / K_<ATP> channel / protein kinase C
Research Abstract

Endogenous adenosine is known to regulate the physiological cardiomyocyte function and to exert a protective action during ischemia and reperfusion. To explore the usefulness of adenosine analogue as a myocardial therapeutic agent, we first examined the electrophysiological effects of adenosine on guinea pig cardiomyocytes in the whole cell patch-clamp configuration. Adenosine shortened the action potential duration (APD) and hyperpolarized the resting membrane potential of atrial but not ventricular myocytes. When atrial cells were preincubated with pertussis toxin (LAP), adenosine did not shorten the APD, confirming that adenosine receptors. couple to the IAP-sensitive GTP-binding protein. Adenosine increased the outward membrane current at 0mV in the presence of nicardipine, an L-type Ca^<2+> channel blocker, which suggests that adenosine elicited the specific K^+-outward current. Adenosine A_1 receptor agonist, N^6-cyclohexyladenosine (CHA), but not A_2 or A_3 receptor agonist, rep … More roduced both the adenosine-induced shortening effect on APD and the increasing effect on the outward membrane current of atrial myocytes. These effects were completely abolished by A_1 receptor antagonist, 8-cyclopentyl-1, 3-dipropylxanthine (DPCPX). Glybenclamide, a KATP channel blocker, completely abolished the CHA-induced shortening effect on APR and also partially abolished the effect on the outward membrane current, which suggests that the effects of CHA are mediated by the activation of K_<ATP> channels on the plasma membrane. Further, the effect of CHA effect, on the outward membrane current was completely abolished by preincubating the cells with chelerythrine, a protein kinase C inhibitor, and was partially,inhibited by 5-hydroxydecanoic acid (5-HD), known as a specific inhibitor of mitochondrial K_<ATP> channels. These results suggest that the shortening of APD caused by adenosine A_1 receptor stimulation was conducted in part through an activation of K_<ATP> channels via protein kinase C and 5-HD-sensitive intracellular signaling pathways, which may be involved in the mechanisms by which adenosine exerts a protective action during ischemia and reperfusion. Less

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Mariko Himeno, Takaharu Ishibashi, Shigeru Nakano, Keisuke Furuya, Toshikazu Kigoshi, Kenzo Uchida, Matomo Nishio: "A practical procedure for achieving a steady state of NOx concentration in plasma : with special reference to the NOx content of Japanese daily food"Tohoku J.Exp.Med.. 199(2). 95-110 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Junko Yoshida, Takaharu Ishibashi, Matomo Nishio: "Antiproliferative effect of Ca^<2+> channel blockers on human epidermoid carcinoma A431 cells"Eur J Pharmacol. 472. 23-31 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takaharu Ishibashi, Junko Yoshida, Matomo Nishio: "New methods to evaluate endothelial function : A search for a marker of nitric oxide (NO) in vivo : Re-evaluation of NOx in plasma and red blood cells and a trial to detect nitrosothiols"J Pharmacol Sci. 93. 409-416 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kaname Kubota, Takaharu Ishibashi, Taku Matsubara, Tomoyuki Hori, Kazuyuki Ozaki, Masaru Yamazoe, Junko Yoshida, Matomo Nishio, Yoshifusa Aizawa: "Effects of 4,4'-diidothiocyanato-stilbene-2,2'-disulfonic acid (DIDS) and chlorpromazine on NO3-transport via anion exchanger in erythrocytes : Inertness of DIDS in whole blood"J Pharmacol Sci. 93. 505-508 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohmi Tashimo, Takaharu Ishibashi, Junko Yoshida, Hideaki Tsuchida, Matomo Nishio: "Interference with Saville's method in determination of low-molecular weight S-nitrosothiols by ultrafiltration"Nitric Oxide. 9. 148-152 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Junko Yoshida, Takaharu Ishibashi, Matomo Nishio: "Antitumor effects of amlodipine, a Ca^<2+> channel blocker, on human epidermoid carcinoma A431 cells in vitro and in vivo."Eur J Pharmacol. 492. 103-112 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mariko Himeno, Takaharu Ishibashi, Shigeru Nakano, Keisuke Furuya, Toshikazu Kigoshi, Kenzo Uchida, Matomo Nishio: "A practical procedure for achieving a steady state of NOx concentration in plasma : with special reference to the NOx content of Japanese daily food."Tohoku J Exp Med. 199(2). 95-110 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Junko Yoshida, Takaharu Ishibashi, Matomo Nishio: "Antiproliferative effect of Ca^<2+> channel blockers on human epidermoid carcinoma A431 cells."Eur J Pharmacol.. 472. 23-31 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takaharu Ishibashi, Junko Yoshida, Matomo Nishio: "New methods to evaluate endothelial function: A search for a marker of nitric oxide (NO) in vivo : Re-evaluation of NOx in plasma and red blood cells and a trial to detect nitrosothiols."J Pharmacol Sci. 93. 409-416 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kaname Kubota, Takaharu Ishibashi, Taku Matsubara, Tomoyuki Hori, Kazuyuki Ozaki, Masaru Yamazoe, Junko Yoshida, Matomo Nishio, Yoshifusa Aizawa: "Effects of 4, 4'-diidothiocyanato-stilbene-2, 2'-disulfonic acid (DIDS) and chlorpromazine on N03-transport via anion exchanger in erythrocytes : Inertness of DIDS in whole blood."J Pharmacol Sci. 93. 505-508 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohmi Tashimo, Takaharu Ishibashi, Junko Yoshida, Hideaki Tsuchida, Matomo Nishio: "Interference with Saville's method in determination of low-molecular weight 3-nitrosothiols by ultrafiltration."Nitric Oxide. 9. 148-152 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Junko Yoshida, Takaharu Ishibashi, Matomo Nishio: "Antitumor effects of amlodipine, a Ca^<2+> channel blocker, on human epidermoid carcinoma A431 cells in vitro and in vivo."Eur J Pharmacol. 492. 103-112 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mariko Himeno, Takaharu Ishibashi, Shigeru Nakano, Keisuke Furuya, Toshikazu Kigoshi, Kenzo Uchida, Matomo Nishio.: "A practical procedure for achieving a steady state of NOx concentration in plasma : with special reference to the NOx content of Japanese daily food"Tohoku J.Exp.Med.. 199(2). 95-110 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Junko Yoshida, Takaharu Ishibashi, Matomo Nishio: "Antiproliferative effect of Ca^<2+> channel blockers on human epidermoid carcinoma A431 cells"Eur J Pharmacol. 472. 23-31 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takaharu Ishibashi, Junko Yoshida, Matomo Nishio: "New methods to evaluate endothelial function : A search for a marker of nitric oxide (NO) in vivo : Re-evaluation of NOx in plasma and red blood cells and a trial to detect nitrosothiols"J Pharmacol Sci. 93. 409-416 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kaname Kubota, Takaharu Ishibashi, Taku Matsubara, Tomoyuki Hori, Kazuyuki Ozaki, Masaru Yamazoe, Junko Yoshida, Matomo Nishio, Yoshifusa Aizawa: "Effects of 4,4'-diidothiocyanato-stilbene-2,2'-disulfonic acid (DIDS) and chlorpromazine on NO3- transport via anion exchanger in erythrocytes : Inertness of DIDS in whole blood"J Pharmacol Sci. 93. 505-508 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohmi Tashimo, Takaharu Ishibashi, Junko Yoshida, Hideaki Tsuchida, Matomo Nishio: "Interference with Saville's method in determination of low-molecular weight S-nitrosothiols by ultrafiltration"Nitric Oxide. 9. 148-152 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mariko Himeno, Takaharu Ishibashi, Shigeru Nakano, Keisuke Furuya, Toshikazu Kigoshi, Kenzo Uchida, Matomo Nishio: "A practical procedure for achieving a steady state of NOx concentration in plasma : with special reference to the NOx content of Japanese daily food"Tohoku J. Exp. Med.. 199(2):(in press). (2003)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi