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The analysis of a novel gene E6DG1 function involving in anchorage dependency

Research Project

Project/Area Number 14570116
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionChiba University

Principal Investigator

SHIRASAWA Hiroshi  Chiba Univ., Graduate School of Medicine, professor, 大学院・医学研究院, 教授 (00216194)

Co-Investigator(Kenkyū-buntansha) KOSEKI Akihiko  Chiba Univ., Graduate School of Medicine, professor, 大学院・医学研究院, 教授 (40225446)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsanchorage dependence / HPV / E6 / Rlp7p / ribosome related protein
Research Abstract

We identified a novel gene, the expression of which is downregulated by the human papillomavirus 16(HPVI6) E6 protein, and designated E6DG1. In this study, we aimed at the analysis of the E6DG1 functions.
The overexpression of the E6DG1 suppressed the anchorage dependency of cancer cells. In contrast, the downregulation of E6DG1 caused the enhancement of the anchorage dependency of several cancer cells, and had no effect on the anchorage dependency of HeLa cells. It was supposed that the pathway involved in E6DG1 may be disturbed in HeLa cells. Both the overexpression and downregulation of E6DG1 also induced apoptosis.
The analysis of E6DG1 revealed that this protein is ribosomal protein related and has homology to 60S L7. The E6DG1 has a domain which is similar to that found in yeast Rlp7p, a ribosomal protein related protein. Our analysis indicated that the E6DG1 has a critical function involved in the biogenesis of ribosome as the yeast Rlp7p.
It was suggested that the E6DG1 is a ribosome-related protein involving in the processing of the ribosome biogenesis, affecting the anchorage dependency. We propose that the E6DG1 might also affect the chromosomal instability through interacting with the cell cycle and apoptosis. This supposed mechanism of the chromosomal instability induced by disturbing the ribosomal biogenesis would provide an insight into a novel mechanism of oncogenesis.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (17 results)

All 2004 2003 2002 Other

All Journal Article (7 results) Publications (10 results)

  • [Journal Article] Lack of evidence for reovirus infection in tissues from patients with biliary atresia and congenital dilatation of the bile duct.2004

    • Author(s)
      Saito, T. et al.
    • Journal Title

      Hepatology 40

      Pages: 203-211

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Broad-spectrum detection of papillomaviruses in bovine teat papillomas and healthy teat skin.2004

    • Author(s)
      Ogawa, T. et al.
    • Journal Title

      Journal of General Virology 85

      Pages: 2191-2197

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Survival regulation in pancreatic cancer cells by c-Jun.2003

    • Author(s)
      Okutomi, Y. et al.
    • Journal Title

      Int.J.Oncol. 23

      Pages: 1127-1134

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth.2002

    • Author(s)
      Sakao, S. et al.
    • Journal Title

      Int.J.Oncol. 21

      Pages: 237-279

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dominant negative MEKK1 inhibits survival of pancreatic cancer cells.2002

    • Author(s)
      Hirano T., et al.
    • Journal Title

      Oncogene 21

      Pages: 5923-5928

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] HPV 16-E6-mediated degradation of intrinsic p53 is compensated by upregulation of p53 gene expression in normal cervical keratinocytes.2002

    • Author(s)
      Kawamata, Y. et al.
    • Journal Title

      Int.J.Oncol. 21

      Pages: 561-567

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dominant negative MEKK1 inhibits survival of pancreatic cancer cells.2002

    • Author(s)
      Hirano, T. et al.
    • Journal Title

      Oncogene 21

      Pages: 5923-5928

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Sakao, S. et al.: "Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth."Int.J.Oncol.. 21. 237-279 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hirano, T. et al.: "Dominant negative MEKK1 inhibits survival of pancreatic cancer cells."Oncogene. 21. 5923-5928 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kawamata, Y. et al.: "HPV 16-E6-mediated degradation of intrinsic p53 is compensated by upregulation of p53 gene expression in normal cervical keratinocytes."Int.J.Oncol.. 21. 561-567 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Komoda, F. et al.: "MEKK1 induces c-Jun complexes that act as negative regulators for cell survival and proliferation of HCC cells."Int.J.Oncol.. 21. 553-559 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okutomi, Y. et al.: "Survival regulation in pancreatic cancer cells by c-Jun."Int.J.Oncol.. 23. 1127-1134 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okamoto, H. et al.: "Dynamic changes in AP-1 transcription factor DNA binding activity in rat brain following administration of antidepressant amitriptyline and brain-derived neurotrophic factor."Neuropharmacology. 45. 251-259 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sakao, S. et al.: "Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth"Int. J. Oncol.. 21. 237-279 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirano, T. et al.: "Dominant negative MEKK1 inhibits survival of pancreatic cancer cells"Oncogene. 21. 5923-5928 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kawamata, Y. et al.: "HPV 16-E6-mediated degradation of intrinsic p53 is compensated by upregulation of p53 gene expression in normal cervical keratinocytes"Int. J. Oncol.. 21. 561-567 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Komoda, F. et al.: "MEKK1 induces c-Jun complexes that act as negative regulators for cell survival and proliferation of HCC cells"Int. J. Oncol.. 21. 553-559 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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