| Project/Area Number |
14570118
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
FURUKAWA Keiko Nagoya University, Graduate School of Medicine, Assistant Professor, 大学院・医学系研究科, 講師 (50260732)
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| Co-Investigator(Kenkyū-buntansha) |
FURUKAWA Koichi Nagoya University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (80211530)
HONDA Takashi Fukushima Medical University, School of Medicine, Associate Professor, 医学部, 助教授 (20165608)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | glycosyltransferase / acidic glycosphingolipids / ganglioside / knock out / degeneration of nervous system / sensory dysfunction / DNA microarray / GM2 / GD2 synthase gene / GD2 / 神経 / 複合型糖脂質 / 糖類合成酵素遺伝子 / DNAチップ / cDNAsubtraction法 |
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| Research Abstract |
We have cloned a number of glycosyltransferase cDNA, products of which are acidic glycosphingolipids (ganglioside), expressed mainly in the nerve system. We have also generated knock out (KO) mice to analyze the biological function of gangliosides. As a result, we found that complex gangliosides play an important role for survival and maintenance of the nervous system. In this study, we analyzed the genes, expression levels of which are different between the wild type and GM2/GD2 synthase gene KO mice using a comprehensive appriache DNA microarray.
We analyzed the chronological alteration of phenotypes in GM2/GD2 synthase gene KO mice and found the degeneration of nervous tissues in the dorsal horn of the spinal cord. Neurological examination revealed progressive sensory dysfunction with aging. We compared the mRNA expression in the spinal cord between the wild type and 20 weeks-old male KO mice by DNA microarray. mRNA expression levels of TGTP, IFI47,IFIT1,IRF7 and IRF1 were higher in KO mice than in the wild type mice. However, the difference in the expression levels was not significant. In the future, we will investigate the relation between these interferon-induced genes and the degeneration of the nervous system, and analyze the expression levels of these genes in older mice after onset of nervous system degeneration. Furthermore, we will prepare the RNA from degenerated sites in the spinal cord then analyze the gene expression profiles in the spinal cord of the wild type and KO mice.
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