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EXPRESSION ANALYSIS OF TRANSCRIPTION FACTOR, ATBF1 AND ZFH4 IN NORMAL DEVELOPING RAT BRAIN

Research Project

Project/Area Number 14570181
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionSHOWA PHARMACEUTICAL UNIVERSITY (2003)
Toyama Medical and Pharmaceutical University (2002)

Principal Investigator

SAKATA Nobuo (2003)  SHOWA PHARMACEUTICAL UNIVERSITY, BIOCHEMISTRY, LECTURER, 薬学部, 講師 (00170598)

川口 誠 (2002)  富山医科薬科大学, 医学部, 助手 (50204699)

Co-Investigator(Kenkyū-buntansha) SASAHARA Masakiyo  TOYAMA MEDICAL & PHARMACEUTICAL UNIVERSITY, PATHOLOGY, PROFESSOR, 医学部, 教授 (20154015)
坂田 宣夫  昭和薬科大学, 薬学部, 講師 (00170598)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsATBF1 / Homeodomain / Zinc Finger / ZFH4 / Central nervous System
Research Abstract

The ATBF1 gene encodes transcription factors containing four homeodomains and multiple zinc finger motifs. However, the gene products have yet to be identified and the role remains unknown in vivo. In this study, we raised an antiserum for ATBF1 and found high levels of expression of ATBF1 in developing rat brain. Western and Northern blot analyses detected a 400 kDa protein and 12.5 kb mRNA in developing rat brain, respectively; both corresponding to ATBF1-A but not the B isoform. The protein was highly expressed in the midbrain and diencephalon and mRNA was highly expressed in the brainstem, mostly in embryo and neonatal brain. immunohistochemistry identified postmitotic neurons in the brainstem as the major site of ATBF1 expression, and the expression levels varied depending on age of and location in the brain. Expression was transient and weak in the precursor cells at early neurogenesis. ATBF1 decreased postnatally, but remained in mature neurons, including those expressing DOPA decarboxylase (DDC). High levels of ATBF1 were expressed in precursor cells in accordance with neurogenesis and were continued to the mature neurons in specific areas such as the inferior colliculus. Expression was not significant from precursor cells to mature neurons in the cerebral cortex and hippocampus. ATBF1 and its Drosophila homolog, Zfh-2, are known to regulate cell differentiation and proliferation via the interaction with either of the basic helix-loop-helix transcription factors, c-myb, or the DDC gene. Together with these reported functions the expression features detected here suggest that ATBF1 may participate in the regulation of neuronal cell maturation or region-specific central nervous system differentiation.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Ishii, Y., M.Kawaguchi, et al.: "ATBF1-A Protein, But Not ATBF1-B, Is Preferentially Expressed In Developing Rat Brain"J Comp Neurol. 465(1). 57-71 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nojiri, S., T.Joh, et al.: "ATBF1 Enhances The Suppression Of STAT3 Signaling By Interaction With PIAS3"Biochem Biophs Res Commun. 314(1). 97-103 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oya, T., Y.L.Zhao, et al.: "Platelet Derived Growth Factor-B Expression Induced After Rat Peripheral Nerve Injuries"Glia. 38(4). 303-312 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zhao, Y.L., K.Takagawa, et al.: "Active Src Expression Is Induced After Rat Peripheral Nerve Injury"Glia. 42(2). 184-193 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takikita-Suzuki, M, M.Haneda, et al.: "Activation Of Src Kinase In Platelet-Derived Growth Factor-B-Dependent Tubular Regeneration After Acute Ischemic Renal Injury"Am J Pathol. 163(1). 277-286 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masuda, T., H.Ogawa, et al.: "Localization And Hormonal Control Of Serine Dehydratase During Metabolic Acidosis Differ Markedly From Those Of Phosphoenolpyruvate Carboxykinase In Rat Kidney"Int J Biochem Cell Biol. 35(8). 1234-1247 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishii, Y., M.Kawaguchi, et al.: "ATBF1-A protein, but not ATBF1-B, is preferentially expressed in developing rat brain."J Comp Neurol. 465-1. 57-71 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nojiri, S., T.Joh, et al.: "ATBF1 enhances the suppression of STAT3 signaling by interaction with PIAS1."Biochem Biophs Res Commun. 314-1. 97-103 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Oya, T., Y.K.Zhao, t al.: "Platelet-derived growth factor-B expression induced after rat peripheral nerve injuries."Glia. 38-4. 303-312 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zhao, Y.K., K.Takagawa, et al.: "Active Src expression is induced after rat peripheral nerve injury."Glia. 42-2. 184-193 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takikita-Suzuki, M., M.Haneda, et al.: "Activation of Src kinase in platelet-derived growth factor-B-dependent tubular regeneration after acute ischemic renal injury."Am J Pathol. 163-1. 277-286 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masuda, T., H.Ogawa, et al.: "Localization and hormonal control of serine dehydratase during metabolic acidosis differ markedly from those of phosphoenolpyruvate carboxykinase in rat kidney"Int J Biochem Cell Biol. 35-8. 1234-1247 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Egawa-Tsuzuki, T., M.Ohno, et al.: "The PDGF B-chain is involved in the ontogenic susceptibility of the developing rat brain to NMDA toxicity."Exo Neurol. 186-1. 89-98 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishii, Y., M.Kawaguchi, et al.: "ATBF1-A Protein, But Not ATBF1-B, Is Preferentially Expressed In Developing Rat Brain"J Comp Neurol. 465(1). 57-71 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nojiri, S., T.Joh, et al.: "ATBF1 Enhances The Suppression Of STAT3 Signaling By Interaction With PIAS3"Biochem Biophs Res Commun. 314(1). 97-103 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Zhao, Y.L., K.Takagawa, et al.: "Active Src Expression Is Induced After Rat Peripheral Nerve Injury"Glia. 42(2). 184-193 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takikita-Suzuki, M., M.Haneda, et al.: "Activation OF Src Kinase In Platelet Derived Growth Factor-B-Dependent Tubular Regeneration After Acute Ischemic Renal Injury"Am J Pathol. 163(1). 277-286 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Masuda, T., H.Ogawa, et al.: "Localization And Hormonal Control Of Serine Dehydratase During Metabolic Acidosis Differ Markedly From Those Of Phosphoenolpyruvate Carboxykinase In Rat Kidney"Int J Biochem Cell Biol. 35(8). 1234-1247 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Egawa-Tsuzuki, T., M.Ohno, et al.: "The PDGF B-Chain Is Involved In The Ontogenic Susceptibility Of The Developing Rat Brain To NMDA Toxicity"Exp Neurol. 186(1). 89-98 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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