Study on suppression of interferon-γ production by nematode antigens
| Project/Area Number |
14570218
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
UCHIKAWA Ryuichi Kyoto Prefectural University of Medicine, Department of Medical Zoology, Research Associate, 医学研究科, 助手 (80145466)
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| Co-Investigator(Kenkyū-buntansha) |
TEGOSHI Tatsuya Kyoto Prefectural University of Medicine, Department of Medical Zoology, Research Associate, 医学研究科, 助手 (40254370)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | nematode antigens / interferon-γ / lymphocytes / rats / mice / Nippostrongylus brasiliensis / Ascaris suum / ガンマ・インターフェロン / Nippostrongylus brasiliensis |
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| Research Abstract |
In this project (2002-2003), we examine interferon (IFN)-γ production suppressing activity in excretory-secretory antigens (ES) derived from Nippostrongylus barasiliensis (Nb) and Ascaris suum (As) adult worms and pointed out the followings;
1.Nb-ES antigen and its partially-purified fraction suppressed IFN-γ production by both rat and mouse T cells.
2.Partially-purified As-ES also suppressed IFN-γ production by rat or mouse T cells, but the body fluid of the worm did not.
3.After ion-exchange and anti-Nb-ES affinity chromatography, we obtained the Nb-ES fraction that has 20 times higher activity in suppression of IFN-γ production by rat T cells comparing with crude Nb-ES antigen.
4.We also characterized the IFN-γ suppressing molecule(s) in Nb-ES.
1)Molecular weight of the IFN-γ suppressing molecule(s) is 50-100kD.
2)The molecule(s) is resistant to 56℃,30 min. but partially sensitive to 95℃,30 min treatment
3)The molecule(s) is sensitive to periodate treatment.
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Report
(3 results)
Research Products
(3 results)
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[Publications] Kuroda, A., Uchikawa, R., Matsuda, S., Yamada, M., Tegoshi, T., Arizono, N.: "Up-regulation of Fas (CD95) and induction of apoptosis in intestinal epithelial cells by nematode-derived molecules"Infection and Immunity. 70. 4002-4008 (2002)
