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Study on anti-tuburculosis DNA vaccines using attenuated Listeria strain as the carrier

Research Project

Project/Area Number 14570234
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionHAMAMATSU UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

NAGATA Toshi  HAMAMATSU UNIV, SCH MED, ASSOCIATE PROFESSOR, 医学部, 助教授 (90275024)

Co-Investigator(Kenkyū-buntansha) AOSHI Taiki  HAMAMATSU UNIV, SCH MED, RESEARCH ASSOCIATE, 医学部, 助手 (10324344)
UCHIJIMA Masato  HAMAMATSU UNIV, SCH MED, RESEARCH ASSOCIATE, 医学部, 助手 (20252174)
KOIDE Yukio  HAMAMATSU UNIV, SCH MED, FULL PROFESSOR, 医学部, 教授 (30126809)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsMycobacterium tuberculosis / Listeria monocytogenes / DNA vaccine / MPB / MPT51 / Antigen 85 complex / attenuated bacteria
Research Abstract

In this study, we showed the induction of specific protective cellular immunity against Mycobacterium tuberculosis employing vaccination with recombinant attenuated Listeria monocytogenes strains. We constructed self-destructing attenuated L. monocytogenes Δ2 strains carrying eukaryotic expression plasmids for Ag85 complex (Ag85A and Ag85B) and MPB/MPT51 molecules.
Infection of these recombinant bacteria allowed expression of the genes in J774A.1 murine macrophage cell line.
The intraperitoneal vaccination of C57BL/6 mice with these recombinant bacteria was capable of inducing purified protein derivative (PPD)-speciflc cellular immunities such as footpad reaction, proliferative responses of splenocytes and interferon-γ production from splenocyles, suggesting the efficacy of the vaccination against mycobacterial infection with these recombinant L. monocytogenes carrying eukaryotic expression plasmids for Ag85 family molecules. Furthermore, intravenous vaccination of these recombinant bacteria carrying expression plasmids for Ag85A, Ag85B, or MPB/MPT51 into BALB/c mice elicited significant protective responses comparable to those evoked by live BCG vaccine. Of note, it is the first report showing MPB/MPT51 is a major protective Ag as well as Ag85A and Ag85B, which have been reported to be major mycobacterial protective Ags.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Miki, K., Nagata, T., Tanaka, T., Kim, Y-H., Uchijima, M., Ohara, N., Nakamura, S., Okada, M., Koide, Y.: "Induction of protective cellular immunity against Mycobacterium tuberculosis by recombinant attenuated self-destructing Listeria monocytogenes strains harboring eukaryotic expression plasmids for Ag85 complex and MPB/MPT51."Infection and Immunity. 72. 2014-2021 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nagata, T, Aoshi, T., Uchijima, M., Suzuki.M., Koide, Y.: "Cytotoxic T-lymphocyte-, and helper T-lymphocyte-oriented DNA vaccination"DNA and Cell Biology. 23. 93-106 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nagata, T., Aoshi, T., Suzuki, M., Uchijima, M., Koide, Y.: "Induction of antigen-specific T-cell subsets by DNA vaccination"Recent Research Developments in Biophysics & Biochemistry. 3. 89-106 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakamura Y., Suda T., Nagata T., Aoshi T., Uchijima M., Yoshida A., Chida K., Koide Y., Nakamura H.: "Induction of protective immunity against an intracellular bacterium using dendritic cells retrovirally transduced with a minigene encoding a CTL epitope."Infection and Immunity. 71. 1748-1754 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小出幸夫, 永田 年: "Annual Review免疫2004"中外医学社. 233-243 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki, M., Aoshi, T., Nagata, T., Koide, Y.: "Identification of H2-D^d-and H2-A^b-restricted T-cell epitopes on a novel protective antigen, MPT51, of Mycobacterium tuberculosis."Infect.Immun.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miki, K., Nagata, T., Tanaka, T., Kim, Y.-H., Uchijima, M., Ohara, N., Nakamura, S., Okada, M., Koide, Y.: "Induction ~of protective cellular immunity against Mycobacterium tuberculosis by recombinant attenuated self-destructing Listeria monocytogenes strains harboring eukaryotic expression plasmids for Ag85 complex and MPB/MPT51."Infect.Immun.. 72(4). 2014-2021

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koide, Y., Nagata, T.: "Induction of anti-tuberculosis cellular immunity with Ag85 DNA vaccines"Annual Review Meneki 2004 (Japanese). 233-243

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Teramoto, K., Kontani, K., Ozaki, Y., Sawai, S., Tezuka, N., Nagata, T., Fujino, S., Itoh, Y., Taguchi, O., Koide, Y., Ohkubo, I., Asai, T., Ogasawara, K.: "DNA encoding a pan-MHC class II peptide analogue augmented antigen-specific cellular immunity and suppressive effects on tumor growth elicited by DNA vaccine immunotherapy."Cancer Res.. 63. 7920-7925 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nagata, T., Aoshi, T., Uchijima, M., Suzuki, M., Koide, Y.: "Cytotoxic T-lymphocyte-, and helper T-lymphocyte-oriented DNA vaccination"DNA Cell Biol.. 23(2). 93-106 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nagata, T., Aoshi, T., Suzuki, M., Uchijima, M., Koide, Y.: "Induction of antigen-specific T-cell subsets by DNA vaccination"Recent Res. Develop. Biophys. Biochem.. 3. 89-106 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishih, A., Baba, S., Nagata, T., Terada, M.: "Intestinal granuloma formation in normal and SCID BALB/c mice infected with Angiostrongylus costariensis."Parasitol.Res.. 89(2). 150-153 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakamura Y., Suda T., Nagata T., Aoshi T., Uchijima M., Yoshida A, Chida K., Koide Y., Nakamura H.: "Induction of protective immunity against an intracellular bacterium using dendritic cells retrovirally transduced with a minigene encoding a CTL epitope."Infect.Immun.. 71(4). 1748-1754 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tozawa, K., Hanai, H., Sugimoto, K., Baba, S., Sugimura, H., Aoshi, T., Uchijima, M., Nagata, T., Koide, Y.: "Evidence for the critical role of interleukin-12 but not interferon-γ in the pathogenesis of experimental colitis in mice."J.Gastroenterol.Hepatol.. 18(5). 578-587 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nagata, T., Miki, K., Kim, Y.-H., Uchijima, M., Ohara, N., Koide, Y.: "Induction of specific cellular immunity against Mycobacterium tuberculosis by recombinant attenuated self-destructing Listeria monocytogenes strains harboring eukaryotic expression plasmids for Ag85 family molecules."Thirty-seventh Research Conference on Tuberculosis and Leprosy. US-Japan Cooperative Medical Science Program. 156-160 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sugimoto, K., Hanai, H., Tozawa, K., Aoshi, T., Uchijima, M., Nagata, T., Koide, Y.: "Curcumin ameliorates trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice."Gastroenterology. 123. 1912-1922 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uchiyama H, Nagata T, Yamada T, Uchijima M, Aoshi T, Suda T, Chida K, Nakamura H, Koide Y.: "Endosomal/lysosomal targeting of a single helper T-cell epitope of an intracellular bacterium by DNA immunisation induces a specific T-cell subset and pertial protective immunity in vivo."FEMS Microbiol. Lett.. 216. 91-97 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nagata, T., Aoshi, T., Suzuki, M., Uchijima, M., Kim, Y-H., Yang, Z., Koide, Y.: "Induction of Protective Immunity to Listeria monocytogenes by Immunization with Plasmid DNA Expressing a Helper T-Cell Epitope That Replaces the Class II-Associated Invariant Chain Peptide of the Invariant Chain"Infect Immun.. 70. 2676-2680 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Nagata, T.Aoshi, M.Suzuki, M.Uchijima, Y.Koide: "Induction of antigen-specific T-cell subsets by DNA vaccination"Recent Research Developments in Biophysics & Biochemistry. 3. 89-106 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Nagata, T.Aoshi, M.Uchijima, M.Suzuki, Y.Koide: "Cytotoxic T-Lymphocyte-, and Helper T-Lymphocyte-Oriented DNA Vaccination"DNA and Cell Biology. 23, 2. 93-106 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Miki, T.Nagata, T.Tanaka, Y.-H.Kim, M.Uchijima, N.Ohara, S.Nakamura, M.Okada, Y.Koide: "Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51"Infection and Immunity. 72, 4(in press). (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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