| Project/Area Number |
14570235
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Nagoya University |
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Principal Investigator |
ITO Hideo Nagoya University, School of Medicine, Professor, 医学部, 教授 (90144162)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAMURA Kumiko Nagoya University, School of Medicine, Research Associate, 医学部, 助手 (30335054)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Gram-negative-rods / metallo-β-lactamase / sodium mercaptoacetic acid (SMA) disk method / nosocomial infection / polymerase chain reaction (PCR) analysis / pulsed field gel electrophoresis / (PFGE) analysis / Extender spectrumβ-lactamase (ESBL) |
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| Research Abstract |
The prevalence of strains producing metallo-β-lactamase among 207 high-level ceftazidime-resistant gram-negative rods (GNRs) was investigated. The strains were isolated in twelve general hospitals in Aichi Prefecture through March 2002 and March 2003. Disk diffusion method using sodium mercapto-acetic acid (SMA) revealed that 60 of them were metallo-β-lactamase producers. The prevalence increased 10.3% in 1997,23.0% in 2001 to 29.0% in this study, and the increase was remarkable among species other than Psudomonas aeruginosa and Serratia marcescens. PCR analysis confirmed that all of the 60 metallo-β-lactamase producing strains detected by SMA disk diffusion method carried the bla_<IMP-1> gene. In this study, SMA disk diffusion method proved useful in detecting metallo-β-lactamase producing at low cost.
In one hospital Extended-spectrum-β-lactamases (ESBLs) producing 13 strains (9 Escherichia coli and 4 Klebsiella oxytoca) were isolated in the internal ward and neonatal intensive care unit (NICU). By pulsed field gel electrophresis (PFGE) each 6 of the 9 strains and each 3 of 4 strains showed the same restriction pattern.
In another hospital 9 methicillin-resistant Staphylococcus aureus (MRSA) strains were isolated in the intensive care unit (ICU). By PFGE 8 of 9 strains were showed the same genotype.
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