Association of retinal antigen Brn 3b with pathogenesis of Behcet's disease

• Project/Area Number: 14570241
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Sapporo Medical University
• Principal Investigator: ISOGAI Hiroshi Sapporo Medical University, School of Medicine, Associated Professor, 医学部, 助教授 (50137436)
• Project Period (FY): 2002 – 2003
• Project Status: Completed (Fiscal Year 2003)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 2002: ¥2,900,000 (Direct Cost: ¥2,900,000)
• Keywords: Brn 3b / Behcet' disease / 網膜 / Brn3b / 遺伝子 / 自己傷害作用 / Bes1 / 組換DNA / HSP / S.sanguis / 合成ペプチド / TGマウス / ブドウ膜炎 / S. sanguis

Research Abstract

About 1kb of Brn 3b DNA was obtained PCR from cDNA library of human embryonic brain. The product was inserted into Escherichia coli and the bacteria produced Brn 3b protein. The bacteria strain was agglutinated by anti-serum to synthetic peptide of Brn 3b. Furthermore, several sera from the patients with BD agglutinated this strain. The results suggested that the Brn 3b protein was correlated with the Behcet's disease.
Now, it is the final stage for developing TG mice which were inserted this DNA.

Research Products

• [Journal Article] Role of Streptococcus sanguis and traumatic factors in Behcet's disease (2003)
  • Author(s): Isogai E, Isogai H, Kotake S, Ohno S, Kimura K, Oguma K.
  • Journal Title: J Appl Res 3(4) Pages: 64-75
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Antimicrobial activity of synthetic human CAP18 peptides to Streptococcus sanguis isolated from pateints with Beheet's disease. (2003)
  • Author(s): Isogai E, Hirata M, Isogai H., Matuse K., Kimura K, Yokota K, Oguma K, Tojo M, Kaneko F, Kotake S, Ohno S.
  • Journal Title: In : Adamantiades-Behcet's disease (Ed : C.C.Zouboulis), Advances in Experimental Medicine and Biology, Vol 528, (Kluwer Academic/Pleunum Publishers, New York) 523 Pages: 195-200
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Role of Streptococcus sanguis and traumatic factors in Behcet's disease (2003)
  • Author(s): Isogai E, Isogai H, Kotake S, Ohno S, Kimura K, Oguma K
  • Journal Title: J Appl Res 3(4) Pages: 64-75
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Antimicrobial activity of synthetic human CAP 18 peptides to Streptococcus sanguis isolated from pateints with Behcet's disease. In : Adam antiades -Behcet's disease (Ed : C.C.Zouboulis) (2003)
  • Author(s): Isogai E, Hirata M, Isogai H., Matuso K., Kimura K, Yokota K, Oguma K, Tojo M, Kaneko F, Kotake S, Ohno S
  • Journal Title: Advances in Experimental Medicine and Biology(Kluwer Academic/Pleunum Publishers, New York) Vol 528 Pages: 195-200
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Antibody cross reactivity from sera of patients with Behcet's disease with synthetic peptides that have homologies with protein from Streptococcus sanguis. (2002)
  • Author(s): Isogai E, Isogai H, Kotake S, Ohno S, I shihara M, Aoki K, Tojc M, Kaneko F, Yokota K, Oguma K
  • Journal Title: J.Appl.Res. 2(3) Pages: 185-191
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Antibody cross reactivity from sera of patients with Behcet's disease with synthetic peptides that have homologies with protein from Streptococcus sanguis. (2002)
  • Author(s): Isogai E, Isogai H, Kotake S, Ohno S, Ishihara M, Aoki K, Tojo M, Kaneko F, Yokota K, Oguma K
  • Journal Title: J.Appl.Res. 2(3) Pages: 185-191
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Emiko Isogai: "Antibody cross reactivity from sera of patients with Behcet's disase with synthetic peptides that have homologies with proteins from Streptococcus sanguis"The Journal of Applied Research. 2(3). 185-191 (2002)
• [Publications] Emiko Isogai: "Role of Streptococcus sanguis and traumatic factors in Behcet's disease"The Journal of Applied Research. 3(1). 64-75 (2003)
• [Publications] Emiko Isogai: "Antimicrobial activity of synthetic human CAP18 peptides to Streptococcus sanguis isolated from patients with Behcet's disease"Advances in experimental medicine and Biology. 528. 195-200 (2003)
• [Publications] E.Isogai: "Antibody Cross Reactivity From Sera of Patients With Behcet's Disease With Synthetic Peptides That Have Homologies With Proteins From Streptococcus sanguis"Journal of Applied Research. 2・3. 1-7 (2002)
• [Publications] E.Isogai: "Role of Streptococcus sanguis and Traumatic Factors in Behcet's Disease"Journal of Applied Research. (In press). (2003)