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Pathogenic Factors for infection in Vibrio vulnificus

Research Project

Project/Area Number 14570242
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionOkayama Prefectural University

Principal Investigator

YAMAMOTO Koichiro  Okayama Prefectural University, Department of Health and Welfare, Professor, 保健福祉学部, 教授 (30158274)

Co-Investigator(Kenkyū-buntansha) HIEMORI Miki  Okayama Prefectural University, Department of Health and Welfare, assistant, 保健福祉学部, 助手 (80326412)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsVibrio vulnificus / infection / gene / chromosome / transposon / 0-antigen / toxin / LPS / バルニフィカス菌 / O抗原
Research Abstract

A Vibrio vulnificus mutant which lacks ability to infect ion-overloaded mice was isolated using a signature-tagged transposon. The 10 kb of the transposon-inserted region of the chromosome was cloned from the gene library and the nucleotide sequence around the insertion region was determined. The transposon-inserted site was found to have homology to Vibrio cholerae UDP-ga]actose-4-epimerase gene region. The region also had homologies to nucleoside-diphosphate sugar epimerase genes, which has an important role in sugar biosynthesis, of Vibrio vulnificus YJ016 and CMCP6 strains. These results suggested that the nock-out gene by the transposon in mutant 0204 may be the gene for biosynthesis of sugar-nucleotides which are precursors necessary for sugar chain formation of lipopolysaccharides. The other mutants are checked for the insertion sites of the transposons. We also carried out pulse-field electrophoresis for V.vulnificus strains using several genes of toxic and pathogenic factors. We found that some of the genes were hybridize to the 210kb NotI fragment, suggesting that some of the pathogenic factors constitute a pathogenic islands.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Takai, K., et al.: "Antibacterial properties of antimicrobial-finished textile products"Microbiol.Immunol.. 46. 75-81 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] MIyazato, T., et al.: "Molecular analysis of VcfQ protein involved in Vibrio cholerae type IV pilus biogenesis"J.Med.Microbiol. 52. 1-6 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Toma C., et al.: "Study of oligomeric and pore-forming states of Vibrio cholerae hemolysin for its crystallization"Proceedings of the 37th Joint Conference on Cholera and Other Bacterial Enteric Infections Panel. 5 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikigai, H., et al.: "Study of oligomeric and pore-forming states of Vibrio cholerae hemolysin for its crystallization"Proceedings of the 37th Joint Conference on Cholera and Other Bacterial Enteric Infections. 3 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikigai, H., et al.: "Comparison of channel activities of water-soluble and membrane-bound oligomers of Vibrio cholerae hemolysin"Proceedings of the 38th Joint Conference on Cholera and Other Bacterial Enteric Infections. 1 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamamoto, K., et al.: "Mutagenesis of Vibrio vulnificus using signature-tagged transposon"Proceedings of the 10th Asian Conference on Diarrhoeal Diseases and Nutrition. 2 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Taniguchi, T., Akeda, Y., Haba, A, Yasuda, Y., Yamamoto, K., Honda, T., Tochikubo, K.: "Gene cluster for assembly of pilus colonization factor antigen III of enterotoxigenic Escherichia coli"Infect.Immun.. 69. 5864-5873 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takai, K., Ohtsuka, T., Senda, Y., Nakao, M., Yamamoto, K., Matsuoka, J., Hirai, Y.: "Antibacterial properties of antimicrobial-finished textile products"Microbiol.Immunol.. 46. 75-81 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyazato, T., Toma, C., Nakasone, N., Yamamoto, K., Iwanaga M.: "Molecular analysis of VcfQ protein involved in Vibrio cholerae type IV pilus biogenesis"J.Med.Microbiol.. 52. 1-6 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takai, K. et al.: "Antibacterial properties of antimicrobial-finished textile products."Microbiol.Immunol.. 46. 75-81 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyazato, T. et al.: "Molecular analysis of VcfQ protein involved in Vibrio cholerae type IV pilus biogenesis."J.Med.Microbiol.. 52. 1-6 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Toma, C. et al.: "Characterization of a mannose-sensitive hemagglutinin type IV pilus variant found in Vibrio cholerae non-01, non-0139."Proceedings of the 37th Joint Conference on Cholera and Other Bacterial Enteric Infections Panel. 5 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ikigai, H. et al.: "Study of oligomeric and pore-forming states of Vibrio cholerae hemolysin for its crystallization."Proceedings of the 37th Joint Conference on Cholera and Other Bacterial Enteric Infections. 3 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ikigai, H. et al.: "Comparison of channel activities of water-soluble and membrane-bound oligomers of Vibrio cholerae hemolysin."Proceedings of the 38th Joint Conference on Cholera and Other Bacterial Enteric Infections. 1 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamamoto, K. et al.: "Mutagenesis of Vibrio vulnificus using signature-tagged transposon."Proceedings of the 10th Asian Conference on Diarrhoeal Diseases and Nutrition. 2 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takai-K, Ohtsuka-T, Senda-Y, Nakao-M, Yamamoto-K, Matsuoka-J, Hirai-Y.: "Antibacterial properties of antimicrobial-finished textile products"Microbiol. Immunol.. 46. 75-81 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Miyazato-T, Toma-C, Nakasone-N, Yamamoto-K, lwanaga-M.: "Molecular analysis of VcfQ protein involved in Vibric cholerae type IV pilus biogenesis"J. Med. Microbiol.. 52. 1-6 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakai, M., Yamamoto, K.: "Isolation of attenuated Vibrio vulnificus by signature-Tagged trasposon"(発表予定).

    • Related Report
      2002 Annual Research Report
  • [Publications] Toma, C., Miyazato, T., KUroki, H., Lu, Y., Ehara, M., Yamamoto, K., Nakasone, N., Iwanaga, M.: "In Proceedings of the 37th Joint Conference on Cholera and Other Bacterial Enteric Infections Panel"Characterization of a mannose-sensitive hemagglutinin type IV pilus variant foun in Vibric cholerae non-O1, non-O139. 34-38 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] kigai, H., Yamada, M., Senda, M., Senda, T., Yamamoto, K., Shimamura, T.: "In Proceedings of the 37th Joint Conference on Cholera and Other Bacterial Enteric Infections Panel"Study of oligomeric and pore-forming states of Vibric cholerde hemolysin for its crystallization. 168-170 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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