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CCL28 Has a Dual Role in Mucosal Immunity as Chemokine with a Broad-Spectrum Antimicrobial Activity

Research Project

Project/Area Number 14570289
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Immunology
Research InstitutionKinki University

Principal Investigator

HIESHIMA Kunio  Kinki University, Dept of Microbiol., Assistant Professor, 医学部, 講師 (10322570)

Co-Investigator(Kenkyū-buntansha) YOSHIE Osamu  Kinki University, Dept of Microbiol., Professor, 医学部, 教授 (10166910)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordschemokine / exocrine gland / salivary gland / antimicrobial activity / plasma cells / mucosal immunity
Research Abstract

CCL28 is a CC chemokine selectively expressed in the salivary glands, mammary glands and colon. We found that CCL28 is constitutively expressed and secreted by the salivary glands and has a dual role as a chemoattractant for B220^<low>CD3^-IgA^+ plasma cells expressing OCR10, receptor for CCL28, and as a potent antimicrobial protein. Serous acinar epithelial cells of human and mouse salivary glands were strongly positive for CCL28 and human whole and parotid saliva contained CCL28 at high concentrations. We also noticed a sequence similarity between the C-terminal region of human CCL28 and human histatin-5, a histidine-rich cationic peptide in human saliva with a potent candidacidal activity. We demonstrated that human and mouse CCL28 had a broad spectrum of antimicrobial activity against Candida albicans, Gram-negative bacteria and Gram-positive bacteria We also demonstrated that the C-terminal 28-amino acid domain of human CCL28 has a selective candklacidal activity as histatin-5. On the other hand, we found no such potent antimicrobial activity with CCL27, which is most closely related to CCL28 and shares CCR10 with CCL28. Finally, we demonstrated that the antimicrobial activity of CCL28 is sensitive to high salt concentrations, as with most other antimicrobial peptides, and displays a rapid induction of membrane permeability in target microbes under scanning electron microscopy In conclusion, CCL28 has a dual role in mucosal immunity as a chemoattractant and as a secreted molecule with a potent antimicrobial activity

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Hieshima, K: "CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity"Journal of Immunology. 170. 1452-1461 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakayama, T: "Cutting edge : profile of chemokine receptor expression on human plasma cells accounts for their efficient recruitment to target tissues"Journal of Immunology. 170. 1136-1140 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 稗島 州雄: "臨床免疫"CCL28の唾液腺における形質細胞走化作用と抗菌作用(in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O: "CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity"Journal of Immunology. 170(3). 1452-1461 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakayama T, Hieshima K, Izawa D, Tatsumi Y, Kanamaru A, Yoshie O: "Cutting edge : profile of chemokine receptor expression on human plasma cells accounts for their efficient recruitment to target tissues"Journal of Immunology. 170(3). 1136-1140 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hieshima, K: "CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity"Journal of Immunology. 170. 1452-1461 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakayama, T: "Cutting edge : profile of chemokine receptor expression on human plasma cells accounts for their efficient recruitment to target tissues"Journal of Immunology. 170. 1136-1140 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 稗島 州雄: "臨床免疫"CCL28の唾液腺における形質細胞走化作用と抗菌作用(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hieshima, K: "CCL28 Has Dual Roles in Mucosal Immunity as a Chemokine with Broad-Spectrum Antimicrobial Activity"Journal of Immunology. 170・3. 1452-1461 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakayama, T: "Cutting edge : profile of chemokine receptor expression on human plasma cells accounts for their efficient recruitment to target tissues"Journal of Immunology. 170・3. 1136-1140 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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